| Identification | Back Directory | [Name]
Methylene Blue | [CAS]
61-73-4 | [Synonyms]
CI 52015
S NO 1038
SWISS BLUE
Chromosmon
Nsc 617593
CI NO 52015
D&CBLUENO.1
BASIC BLUE 9
Schultz 1038
Urolene Blue
METHYLENE BLUE
MethyleneBlueGr
CI BASIC BLUE 9
External Blue 1
Maxilon Blue Sg
Conbasic Blue N
Methylene Blue A
Methylene Blue D
Methylene Blue G
Methylene Blue Gz
Methylene Blue Mb
Methylene Blue Nf
Methylene Blue Nz
Methylene Blue Sg
Methylene Blue Sp
Methylene Blue Zx
Methylene Blue Bd
Methylene Blue Bp
Methylene Blue Bz
Methylene Blue FZ
METHYLENE BLUE 2B
CALCOZINE BLUE ZF
METHYLENE BLUE ZF
METHYLENE BLUE BX
Methylene Blue Bpc
Methylene Blue Bba
Methylene Blue 2bf
Methylene Blue 2bn
Methylene Blue 2bp
Methylene Blue Hgg
Methylene Blue Iad
Methylene Blue Jfa
Tetramethylthionine
Methylene Blue p.a.
Methylene Blue
SANDOCRYL BLUE B-RL
METHYLENE BLUE STAIN
METHYLENE BLUE-ALPHA
abcolmethyleneblue2b
METHYLENE BLUE 3 H2O
Leather pure blue hb
Methylenium Ceruleum
Mitsui Methylene Blue
METHYLENE BLUE POWDER
METHYLENE BLUE: 98.5%
EHRLICH'S REAGENT III
NEISSER'S SOLUTION IA
METHYLENE BLUE REAGENT
DNA-DYE METHYLENE BLUE
BORON 1,000PPM FOR ICP
Ext D And C Blue No. 1
Basovict Methylene Blue
Duasyn Basic Blue IAD01
METHYLENEBLUE,ANHYDROUS
METHYLENE BLUE SOLUTION
METHYLTHIONINE CHLORIDE
METHYLTHIONINI CHLORIDUM
NEISSER'S METHYLENE BLUE
METHYLENE BLUE, CHLORIDE
METHYLENE BLUE INDICATOR
METHYLENE BLUE, LOEFFLER
Basicblue9=Methyleneblue
Ravi Methylene Blue Z.F.
FUCHSIN BASIC MICROSCOPY
MethylthioniniuM choride
Methylene Blue, HARLECO?
Yamamoto Methylene Blue B
METHYLENE BLUE POLYCHROME
METHYLENE BLUE LOEFFLER'S
LOEFFLER'S METHYLENE BLUE
METHYLTHIONINIUM CHLORIDE
METHYLENE BLUE, ZINC-FREE
abcolmethyleneblue2bznfree
Yamamoto Methylene Blue Zf
Methylene blue (CI x 52015)
Dycosbasic Methylene Blue BB
TETRAMETHYLTHIONINE CHLORIDE
Methylene Blue,pure,certified
Basic Blue 9,METHYLENE BLUE BB
METHYLENE BLUE, CHLORIDE, KOCH
METHYLENE BLUE, LOEFFER FORMULA
Methylene Blue, certified, pure
Methylene Blue 2B, Basic blue 9
Hidaco methylene blue salt free
LISTERIA ENRICHMENT SUPLEM. 24ML
METHYLENE BLUE, LOEFFLER FORMULA
METHYLENE BLUE ALKALINE LOEFFLER
VISIBLELIGHT-EXCITEDMETHYLENEBLUE
METHYLENE BLUE ACCORDING TO EHRLICH
METHYLENE BLUE ACCORDING TO LOFFLER
Methylene Blue, certified, pure 25GR
Methylene blue, C.I. 52015, extra pure
METHYLENE BLUE POLYCRHOME (MCFADYEN'S)
METHYLENE BLUE SOLUTION 0.05% SOLUTION
METHYLENE BLUE SOLUTION, 0.5% IN WATER
METHYLENE BLUE TABLETS FOR MILK TESTING
METHYLENE BLUE, 1% W V AQUEOUS SOLUTION
Methylene Blue, for biological staining
METHYLENE BLUE (C.I. 52015) FOR MICROSCO
METHYLENE BLUE (C.I. 52015) REAG. PH EUR
Methylene Blue solution, 1% w/v in water
3,7-BIS(DIMETHYLAMINO)PHENAZATHIONIUMHCL
METHYLENE BLUE 0.05 WT. % SOLUTION &
METHYLENE BLUE WATER AND ALCOHOL SOLUBLE
METHYLENE BLUE STAIN SOLUTION, LOEFFLER'S
METHYLTHIONINIUM CHLORIDE(METHYLENE BLUE)
METHYLENE BLUE TECHNICAL GRADE C.I. 52015
Methylene blue, C.I. 52015, for microscopy
Methylene Blue Chloride (Certifiable Stain)
Methylene Blue – potassium hydroxide solution
METHYLENE BLUE SOLUTION ACCORDING TO LOEFFLER
METHYLENE BLUE, 0.05 WT. % SOLUTION IN W ATER
3,7-BIS(DIMETHYLAMINO)PHENAZATHIONIUM CHLORIDE
METHYLENE BLUE, 100X DNA STAIN ULTRA PURE GRADE
Methylene blue, carbol solution, for microscopy
lǒffler's alkaline methylene blue stain solution
3,7-BIS(DIMETHYLAMINO)-5-PHENOTHIAZINIUM CHLORIDE
Loeffler’s alkaline methylene blue stain solution
METHYLENE BLUE SOLUTION ALKALINE ACC. TO LOEFFLER
Ehrlichs reagent III, Loefflers Methylene Blue
methylene blue solution alkaline according to lǒffler
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride
METHYLENE BLUE TRIHYDRATE HIGH PURITY BIOLOGICAL STAIN
[Ion association reagent for spectrophotoMetric analysis]
Phenothiazin-5-iuM,3,7-bis(diMethylaMino)-, chloride (1:1)
METHYLENE BLUE CONCENTRATE ACC. TO EHR-L ICH, F. MICROSCOPY
Methylene Blue solution alkaline according to Loeffler
METHYLENE BLUE SOLUTION ACCORDING TO LOE FFLER FOR MICROSCOPY
Methylene Blue Solution (Methanol Solution)[for Cell Staining]
3,7-Bis(dimethylamino)-5-phenothiazinium Chloride
Basic Blue 9
METHYLENE BLUE POLYCHROME (MCFADYEN'S) 1% AQUEOUS STAINING SOLUTION
Methylene Blue [Ion association reagent for spectrophotometric analysis]
METHYLENE BLUE CONCENTRATED, WATERY,ACCO RDING, TO EHRLICH FOR MICROSCOPY
METHYLENE BLUE CERTIFIED (C.I. 52015) FOR STAINING AND REAGENT FOR SPECTROSCOPY USE
Methylene Blue, An ethidium bromide alternative for the visualization of nucleic acids in gels
Loefflers alkaline methylene blue stain solution, Methylene Blue - potassium hydroxide solution
METHYLENE BLUE INDICATOR, HIGH PURITY BIOGICAL STAIN, SPECTROPHOTOMETRIC ANALYSIS & MICROBIOLOGY GRADE 98.5%, C.I.NO 52015 | [EINECS(EC#)]
200-515-2 | [Molecular Formula]
C16H18ClN3S | [MDL Number]
MFCD00012111 | [MOL File]
61-73-4.mol | [Molecular Weight]
319.85 |
| Chemical Properties | Back Directory | [Appearance]
green fine crystalline powder | [Melting point ]
190 °C (dec.)(lit.)
| [density ]
1.0 g/mL at 20 °C
| [refractive index ]
n20/D1.347 | [Fp ]
45 °C | [storage temp. ]
Store at RT | [solubility ]
Soluble in water, ethanol, ethylene glycol, methyl cellosolve | [Colour Index ]
52015 | [form ]
Liquid | [pka]
2.6, 11.2(at 25℃) | [color ]
Green | [Specific Gravity]
0.98 | [Odor]
Odorless | [Water Solubility ]
40 g/L (20 ºC) | [λmax]
661 nm | [Merck ]
14,6060 | [BRN ]
3641570 | [Biological Applications]
Detecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas,erysipelas,hidradenitis suppurativa,inflammation,skin diseases | [NIST Chemistry Reference]
Methylene blue(61-73-4) | [EPA Substance Registry System]
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride(61-73-4) |
| Hazard Information | Back Directory | [Chemical Properties]
green fine crystalline powder | [Uses]
A useful inhibitor of cGMP-mediated processes and a useful stain | [Definition]
ChEBI: An organic chloride salt having 3,7-bis(dimethylamino)phenothiazin-5-ium as the counterion. A commonly used dye that also exhibits antioxidant, antimalarial, antidepressant and cardioprotective properties. | [Hazard]
Toxic by ingestion. | [Biological Activity]
Biological stain and redox indicator. Inhibits tau filament formation (IC 50 = 1.9 μ M). Also inhibits soluble guanylyl cyclase. | [Preparation]
MethyleneBlue45,C.I. 52 015 [61-73-4], is obtained by oxidation of 4-aminodimethylaniline in the presence ofsodium thiosulfate to give the quinonediiminothiosulfonic acid, reaction with dimethylaniline, oxidation to the indamine, and cyclization to give the thiazine. | [General Description]
Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria. | [Biochem/physiol Actions]
Methylene blue (MB) is a highly recognized histological dye. It plays a vital role in microbiology and pharmacology. It has been employed in staining living organisms and in methemoglobinemia treatment. MB exhibits maximum absorption at 664nm. Exposure to methylene blue leads to various health hazards such as increased heart rate, vomiting, shock, Heinz body formation, cyanosis, jaundice, quadriplegia and tissue necrosis in humans | [Clinical Use]
Methylene blue is 3,7-bis(dimethylamino)-phenazathioniumchloride (Urised). The compound occurs as a dark greencrystalline powder with a metallic appearance that is solublein water (1:25) and alcohol (1:65).
Methylene blue has weak antiseptic properties that makeit useful for the treatment of cystitis and urethritis. The actionof methylene blue is considered to be bacteriostatic.The compound colors the urine and stool blue green. | [Safety Profile]
Poison by ingestion,intraperitoneal, intravenous, and subcutaneous routes.Human systemic effects: cyanosis, blood changes.Experimental reproductive effects. Mutation datareported. When heated to decomposition it emits verytoxic fumes of NOx, SOx, and | [Veterinary Drugs and Treatments]
Methylene blue is used primarily for treating methemoglobinemia
secondary to oxidative agents (nitrates, chlorates) in ruminants. It
is also employed occasionally as adjunctive or alternative
therapy
for cyanide toxicity.
Intra-operative methylene blue is also being used to preferentially
stain islet-cell tumors of the pancreas
in dogs in order to aid in
their surgical removal or in determining the animal’s prognosis. | [storage]
Store at -20°C | [Purification Methods]
Crystallise the chloride from 0.1M 664 2HCl (16mL/g), the crystals are separated by centrifugation, washed with chilled EtOH and diethyl ether, and dried under vacuum. Crystallise it from 50% aqueous EtOH, wash it with absolute EtOH, and dry it at 50-55o for 24hours. It has also been crystallised from *benzene/MeOH (3:1). It has been salted out with NaCl from a commercial concentrated aqueous solution, then crystallised from water, and dried at 100o in an oven for 8-10hours. [Beilstein 27 III/IV 5152.] |
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