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2321-07-5

中文名称 荧光素
英文名称 Fluorescein
CAS 2321-07-5
EINECS 编号 219-031-8
分子式 C20H12O5
MDL 编号 MFCD00005050
分子量 332.31
MOL 文件 2321-07-5.mol
更新日期 2024/03/25 09:11:41
2321-07-5 结构式 2321-07-5 结构式

基本信息

中文别名
2-(6-羟基-3-氧代-3H-呫吨-9-基)苯甲酸
萤光橙黄
萤光红
萤光黄
萤光素
荧光酯
荧光橙红
荧光黄
荧光素,水溶
黄 7 [CI 45350:1]
荧光素99%
螢光黃
二酚酞
荧光素
2-(6-羟基-3-氧代-3H-吨-9-基)苯甲酸
荧光素, 90+%
英文别名
ACID YELLOW 73
CI 45350
CI 45350.1
CI 45350:1
CI NO 45350
D AND C YELLOW NO 7
FLUORESCEIN
FLUORESCEIN, ALCOHOL SOLUBLE
RESORCINOL PHTHALEIN
S NO 880
SOLVENT YELLOW 94
11712yellow
2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-benzoicaci
3,6-dihydroxyspiro(xanthene-9,3’-phthalide)
3,6-fluorandiol
3’,4’-dehydroxyfluoran
3’,6’-dihydroxy-fluora
3’,6’-dihydroxyfluoran
3’,6’-dihydroxyspiro(isobenzofuran-1(3h),9’(9h)-xanthen)-3-one
3’,6’-fluorandiol
所属类别
生物化工:生物染料

物理化学性质

外观性状橙黄或浅橙红色结晶粉末。熔点314-316℃(分解)。溶于热乙醇、冰醋酸、碱溶液,呈绿色萤光,不溶于水、苯、氯仿和乙醚。
熔点320 °C(lit.)
沸点429.44°C (rough estimate)
密度1.2739 (rough estimate)
折射率1.5000 (estimate)
储存条件−20°C
溶解度不溶于水、乙醚、苯、氯仿;溶于乙醇、甲醇、丙酮、乙酸乙酯、N,N-二甲基甲酰胺
色指数45350
酸度系数(pKa)2.2, 4.4, 6.7(at 25℃)
形态粉末
颜色红色到橙色
酸碱指示剂变色ph值范围Pink uorescence (4.0) to green uorescence (6.0)
水溶解性insoluble
最大波长(λmax)493.5nm, 496nm, 460nm, 515nm
Merck14,4159
BRN94324
稳定性稳定的。易燃。与强氧化剂不相容。
生物领域应用Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
主要应用Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
LogP2.980 (est)
EPA化学物质信息Fluorescein (2321-07-5)

安全数据

危险性符号(GHS)
GHS07
警示词警告
危险性描述H319
防范说明P305+P351+P338
危险品标志Xi
危险类别码R43-R36
WGK Germany3
RTECS号LM5075000
TSCAYes
海关编码32042000

应用领域

用途一
汞溴红的中间体。萤光光度分析试剂,测定氯、溴、碘、硫离子;氧化-还原指示试剂;生物染色剂。
用途二
荧光光度分析硫离子,试验溴,滴定氯、溴和碘。吸附指示剂,氧化还原指示剂。

制备方法

方法一
用邻苯二甲酸酐与间苯二酚在硫酸参与下反应制得。
荧光素价格(试剂级)
报价日期产品编号产品名称CAS号包装价格
2024/01/25HY-D0251荧光素
Fluorescein
2321-07-5250mg500元
2024/01/25HY-D0251荧光素
Fluorescein
2321-07-510mM * 1mLin DMSO550元
2024/01/25HY-D0251荧光素
Fluorescein
2321-07-51g650元

常见问题列表

生物活性
Fluorescein (Resorcinol phthalein) 是一种合成的有机光敏染料化合物。Fluorescein 是荧光示踪剂,可以应用于医学和生物学以及肿瘤感染的组织。
体外研究

Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays.
The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent.

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