300815-04-7
中文名称
Apcin
英文名称
Apcin
CAS
300815-04-7
分子式
C13H14Cl3N7O4
分子量
438.65
MOL 文件
300815-04-7.mol
更新日期
2024/05/07 11:47:11
300815-04-7 结构式
基本信息
中文别名
2,2,2-三氯-1-(嘧啶-2-基氨基)乙基氨基甲酸2-(2-甲基-5-硝基-1H-咪唑-1-基)乙基 英文别名
ApcinApcin >=98% (HPLC)
3-(2-Methyl-5-nitroimidazol-1-yl)-N-(2,2,2-trichloro-1-phenylaminoethyl)propionamide
2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl 2,2,2-trichloro-1-(pyrimidin-2-ylamino)ethylcarbamate
Carbamic acid, [2,2,2-trichloro-1-(2-pyrimidinylamino)ethyl]-, 2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl ester (9CI)
物理化学性质
密度1.68±0.1 g/cm3(Predicted)
储存条件room temp
溶解度DMF: 25 mg/ml; DMSO: 25 mg/ml; DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml
酸度系数(pKa)9.67±0.46(Predicted)
形态粉末
颜色白色至米色
Apcin价格(试剂级)
| 报价日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
| 2024/04/30 | HY-110287 | Apcin Apcin | 300815-04-7 | 5mg | 1000元 |
| 2024/04/30 | HY-110287 | Apcin Apcin | 300815-04-7 | 10mM * 1mLin DMSO | 1100元 |
| 2024/04/30 | S9605 | Apcin Apcin | 300815-04-7 | 5mg | 1270.46元 |
常见问题列表
生物活性
Apcin (APC inhibitor) 是一种有丝分裂的 anaphase-promoting complex/cyclosome (APC/C) 的E3连接酶活性的抑制剂，其可与Cdc20结合并竞争性地抑制含D-box底物的泛素化。靶点
| Target | Value |
|
APC/C
() | |
|
CDC20
() |
体外研究
Apcin (25-50 μM; 48 hours) significantly increases MM apoptosis combining with proTAME (6, 12 μM).
Apcin (25 μM; 2-14 hours) induces slippage more slowly.
Apcin (50-200 μM) stabilizes cycB1-NT and securin most effectively, with somewhat weaker effects against full-length cyclin B1.
Apcin (1.5-200 μM; 18 hours) synergizes with proTAME (a cell-permeable TAME prodrug) to prolong mitotic duration in RPE1 cells.
Apoptosis Analysis
| Cell Line: | HMCLs LP-1 and RPMI-8226 cells |
| Concentration: | 25, 50 μM |
| Incubation Time: | 48 hours |
| Result: | Had minor effects on multiple myeloma (MM) cells alone and significantly increased MM apoptosis combining with proTAME (6, 12 μM). |
Western Blot Analysis
| Cell Line: | HeLa cells with Nocodazole (100 nM) |
| Concentration: | 25 μM |
| Incubation Time: | 2-14 hours |
| Result: | Induced slippage more slowly, as indicated by Cdc27 dephosphorylation and a reduction in cyclin B1, securin and phosphoH3 levels beginning 4-6h after mitotic entry. |