脱氧胆酸钠 MSDSSodium deoxycholate
以9α-羟基雄-4-烯-3,17-二酮（化合物25）为起始原料，氢化、提纯后,得化合物26，惰性气氛下向化合物26的DCM溶液中添加硫酸，搅拌，反应完全后洗涤、真空蒸馏，得灰白色固体化合物27，在-40℃至-45℃惰性气氛下，向化合物27的THF溶液中加入三叔丁基氧氢化铝锂，搅拌反应完全后，添加HCl溶液淬灭反应混合物，分离各相并萃取水层，洗涤、蒸发，得化合物28。向溴化乙基三苯基膦的THF溶液中逐滴添加叔丁醇钾，搅拌1h后向体系中加入化合物28，使反应完全，冰水淬灭，MTBE萃取水层，洗涤并合并有机萃取物，真空浓缩有机层，MTBE纯化粗物质，过滤，蒸馏，制备出化合物29，加入三乙胺、DMAP和乙酸酐，反应得化合物30，经由化合物84或85转化为化合物32，向化合物32的乙酸乙酯溶液中添加TBHP水溶液，0℃下缓慢添加次氯酸钠，搅拌反应，分离并萃取，合并有机层，亚硫酸钠溶液处理2h后用氯铬酸吡啶鎓处理有机层，洗涤并蒸发有机层，纯化得浅黄色固体状化合物24，用Pd/C氢化还原环上的双键，并用三叔丁氧基氢化铝锂还原羰基得到化合物34，碱性条件下水解得脱氧胆酸粗品，纯化即制得脱氧胆酸，NaOH碱化制得脱氧胆酸钠。其合成路线如下图所示。
Deoxycholic acid (DCA) and chenoDeoxycholic acid (CDCA), as the common ingredients of duodenal reflux, act synergistically in many physiological and pathological processes. The cells are repeatedly exposed to 100 μM CDCA and Deoxycholic acid at pH 5.5 for up to 120 min. To simulate chronic local recurrent disease in vitro, the gastric cancer cell line MGC803 is exposed to acidified medium (pH 5.5) containing 100 μM Deoxycholic acid and CDCA. An untreated log-growth MGC803 cell line is generated to be used as a control in normal pH media. After daily 10 min exposure to the acidified bile acids for 60 weeks, MGC803-resistant cells are able to survive and proliferate after 120 min exposure.