NSC 95397
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- CAS号:
- 93718-83-3
- 英文名:
- NSC 95397
- 英文别名:
- NSC 95397;Cdc25 Inhibitor IV, NSC 95397;2,3-BIS[(2-HYDROXYETHYL)THIO]-1,4-NAPHTHOQUINONE;2,3-BIS(2-HYDROXYETHYLTHIO)-1,4-NAPHTHALENEDIONE;2,3-BIS[(2-HYDROXYETHYL)THIOL]-1,4-NAPHTHOQUINONE;1,4-Naphthalenedione, 2,3-bis[(2-hydroxyethyl)thio]-;Cdc25 Inhibitor IV, NSC 95397 - CAS 93718-83-3 - Calbiochem;Apoptosis,NSC-95397,Inhibitor,NSC 95397,inhibit,NSC95397,Phosphatase
- 中文名:
- NSC 95397
- 中文别名:
- 化合物NSC 95397;2,3-双(2-羟基乙硫基)-1,4-萘二酮;NSC 95397,CDC25双特异性磷酸酶抑制剂;CDC25 INHIBITOR IV, NSC 95397 CALBIOCHEM
- CBNumber:
- CB7402539
- 分子式:
- C14H14O4S2
- 分子量:
- 310.39
- MOL File:
- 93718-83-3.mol
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NSC 95397化学性质
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储存条件:
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-20°C
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溶解度:
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DMSO: 16 mg/mL
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形态:
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Orange solid
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颜色:
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orange, solid
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稳定性:
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Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
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NSC 95397性质、用途与生产工艺
NSC 95397 是一种有效的、选择性 Cdc25 双特异性磷酸酶抑制剂，对于 Cdc25A、Cdc25B 和 Cdc25C 的Ki值分别为32 nM、96 nM 和 40 nM。NSC 95397 对 hCdc25A、hCdc25C 和 hCdc25B 的IC50值分别为22.3 nM、56.9 nM和125 nM。NSC 95397 抑制 mitogen-activated protein kinase phosphatase-1 (MKP-1) 和增殖，并通过 MKP-1 和 ERK1/2 途径诱导结肠癌细胞凋亡。
| Target | Value |
MKP-1
()
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Cdc25A
(Cell-free assay)
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22.3 nM
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Cdc25A
(Cell-free assay)
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32 nM(Ki)
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Cdc25C
(Cell-free assay)
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40 nM(Ki)
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Cdc25B
(Cell-free assay)
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56.9 nM
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NSC 95397 (0, 10, and 20 µM; 24 hour) decreases the cell viability of three colon cancer cell lines SW480, SW620, and DLD-1 in a concentration-dependent manner.
NSC 95397(10 μM; 24 hour) upregulates p21 while downregulates CDK4 and CDK6 were d in all three colon cancer cell lines SW480, SW620, and DLD-1 cells.
NSC 95397 (10 μM; 24 hour) reduces the phosphorylation of retinoblastoma protein (Rb) on Ser795 and Ser807/811.
NSC 95397 (20 μM; 24 hours) significantly increases cleaved caspase-9, -3, -7 and PARP levels.
NSC 95397 (10 μM; 6 hours) enhances the phosphorylation of its downstream ERK1/2 at Thr202/Tyr 204.
Cell Viability Assay
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Cell Line:
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Human colon cancer cell lines, SW480, SW620, and DLD-1
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Concentration:
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0, 10, and 20 µM
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Incubation Time:
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24 hours
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Result:
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The IC
50
values of NSC 95397 for the cell growth of SW480, SW620, and DLD-1 cells were 9.9, 14.1 and 18.6 μM, respectively.
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Western Blot Analysis
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Cell Line:
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SW480, SW620, and DLD-1 cells
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Concentration:
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10 μM
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Incubation Time:
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24 hours
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Result:
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p21 was upregulated while CDK4 and CDK6 were downregulated.
Rduced the phosphorylation of Rb on Ser795 and Ser807/811
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NSC 95397
上下游产品信息
上游原料
下游产品
| 更新日期 | 产品编号 | 产品名称 | CAS编号 | 包装 | 价格 |
|---|
| 2024/04/30 | HY-108543 | NSC 95397
NSC 95397 | 93718-83-3 | 5mg | 1200元 |
| 2024/04/30 | HY-108543 | NSC 95397
NSC 95397 | 93718-83-3 | 10mM * 1mLin DMSO | 1320元 |
93718-83-3, NSC 95397 相关搜索:
- Protein Phosphatase
- 药靶配体
- Cdc25 dual specificity phosphatase (Cdc25)
- Cell Biology
- Cell Signaling and Neuroscience
- BioChemical
- Kinase/Phosphatase Biology
- Serine/Threonine Phosphatase Inhibitors
- CDC25 INHIBITOR IV, NSC 95397 CALBIOCHEM
- NSC 95397,CDC25双特异性磷酸酶抑制剂
- 化合物NSC 95397
- 2,3-双(2-羟基乙硫基)-1,4-萘二酮
- 93718-83-3
- Apoptosis,NSC-95397,Inhibitor,NSC 95397,inhibit,NSC95397,Phosphatase
- Cdc25 Inhibitor IV, NSC 95397 - CAS 93718-83-3 - Calbiochem
- NSC 95397
- Cdc25 Inhibitor IV, NSC 95397
- 1,4-Naphthalenedione, 2,3-bis[(2-hydroxyethyl)thio]-
- 2,3-BIS[(2-HYDROXYETHYL)THIOL]-1,4-NAPHTHOQUINONE
- 2,3-BIS[(2-HYDROXYETHYL)THIO]-1,4-NAPHTHOQUINONE
- 2,3-BIS(2-HYDROXYETHYLTHIO)-1,4-NAPHTHALENEDIONE