ChemicalBook >> CAS DataBase List >>Collagenase

Collagenase

CAS No.
9001-12-1
Chemical Name:
Collagenase
Synonyms
FIBROBLAST;Collagenase IV;Collagenase clostridium histolyticum;Santyl;INTERSTITIAL;Collagenase A;Collagenase II;COLLAGENASE TYPE XI;COLLAGENASE TYPE VII HIGH PURITY;CLGN
CBNumber:
CB2112176
Molecular Formula:
C60H100N2
Molecular Weight:
849.4494
MDL Number:
MFCD00130830
MOL File:
9001-12-1.mol
Last updated:2024-03-28 16:55:20

Collagenase Properties

storage temp. -20°C
solubility Soluble in aqueous buffers.
form powder
color light brown
PH 7.0
Merck 2481
CAS DataBase Reference 9001-12-1
EWG's Food Scores 1
NCI Dictionary of Cancer Terms collagenase; fibroblast
FDA UNII 9X7O8V25IT
NCI Drug Dictionary collagenase
ATC code D03BA02,D03BA52,M09AB02
EPA Substance Registry System Collagenase (9001-12-1)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictograms
GHS07,GHS08
Signal word  Danger
Hazard statements  H315-H319-H334-H335
Precautionary statements  P261-P264-P280-P284-P304+P340+P312-P342+P311
Hazard Codes  Xn
Risk Statements  36/37/38-42
Safety Statements  22-24-26-36/37
WGK Germany  1
10-21
HS Code  3507907000

Collagenase price More Price(99)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich COLLA-RO Collagenase A from Clostridium histolyticum 9001-12-1 100mg $86.8 2024-03-01 Buy
Sigma-Aldrich COLLA-RO Collagenase A from Clostridium histolyticum 9001-12-1 500mg $324 2024-03-01 Buy
Sigma-Aldrich COLLA-RO Collagenase A from Clostridium histolyticum 9001-12-1 2.5g $1020 2024-03-01 Buy
Sigma-Aldrich C8176 Collagenase from Clostridium histolyticum Sigma Blend Type L, ≤1.0?FALGPA units/mg solid 9001-12-1 5G $5710 2024-03-01 Buy
Sigma-Aldrich C2799 Collagenase from Clostridium histolyticum powder, suitable for cell culture, ≥4?FALGPA units/mg solid, high purity, ≥700?CDU/mg solid (CDU = collagen digestion units) 9001-12-1 7500units $228 2024-03-01 Buy
Product number Packaging Price Buy
COLLA-RO 100mg $86.8 Buy
COLLA-RO 500mg $324 Buy
COLLA-RO 2.5g $1020 Buy
C8176 5G $5710 Buy
C2799 7500units $228 Buy

Collagenase Chemical Properties,Uses,Production

Description

Collagenases are a category of enzymes that can digest native collagen, the major fibrous component of animal extracellular connective tissue. Collagenases can be produced by some bacterias such as Clostridium  and can also be made by the animal body as part of its normal immune response. They normally target the connective tissue in muscle cells and other body organs. They can break peptide bonds within collagen and help create collagen by cleaving pro-collagen, collagen's precursor, once it is secreted by the cell. Bacterial collagenases exhibit broader substrate specificity than animal collagenases and unlike animal collagenases that split collagen in its native triple-helical conformation, bacterial collagenase can degrade both water-insoluble native collagens and water-soluble denatured ones. In addition, bacterial collagenase can attack almost all collagen types, and is able to make multiple cleavages within triple helical regions.  Collagenases have a molecular weight ranging from 68 kilodaltons (kDa) to 130 Kda due to differing sizes based on the class to which the isolate belongs. The optimal pH for stability ranges from approximately 6.3 to 7.5 with a theoretical isoelectric point of 5.62. Numerous compounds and molecules have the capability of acting as collagenases activator or inhibitor. For example, Ca2+ and Zn2+ ions are two known activators. On the other hand, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), cysteine, histidine, dithiothreitol (DTT), 2-mercaptoethanol, o-phenanthroline, Hg2+, Pb2+, Cd2+, Cu2+, and Zn2+ are all known inhibitors of collagenase activity.

References

1.https://en.wikipedia.org/wiki/Collagenase
2.http://www.wisegeek.com/what-is-collagenase.htm
3.http://www.rockland-inc.com/collagenase.aspx
4.http://www.worthington-biochem.com/cls/default.html

Uses

This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

Uses

In investigation of the structure and biosynthesis of collagen; in dispersion of cells for tissue culture studies.

Uses

Collagenase, Type I, Clostridium histolyticum is used as a powder and solvent injection kit for the treatment of dupuytren's contracture.

Definition

A rare proteinase that catalyzes the hydrolysis of collagen. Collagenase activity has been demonstrated in several venoms of crotalid and viperid snakes and it occurs in certain species of clostridium.

General Description

Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.

Biochem/physiol Actions

Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.

Purification Methods

Collagenase is purified by using N-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986].

Collagenase Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 158)Suppliers
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View Lastest Price from Collagenase manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Collagen-Peptide pictures 2024-03-28 Collagen-Peptide
9001-12-1
US $0.00 / kg 1kg 99% 20tons Shaanxi TNJONE Pharmaceutical Co., Ltd
Collagenase pictures 2024-02-27 Collagenase
9001-12-1
US $50.00-30.00 / kg 1kg 99% 10 tons Hebei Yanxi Chemical Co., Ltd.
Collagenase pictures 2023-10-26 Collagenase
9001-12-1
US $20.00-6.00 / kg 1kg 0.99 20 tons Hebei Yanxi Chemical Co., Ltd.
  • Collagen-Peptide pictures
  • Collagen-Peptide
    9001-12-1
  • US $0.00 / kg
  • 99%
  • Shaanxi TNJONE Pharmaceutical Co., Ltd
  • Collagenase pictures
  • Collagenase
    9001-12-1
  • US $50.00-30.00 / kg
  • 99%
  • Hebei Yanxi Chemical Co., Ltd.
  • Collagenase pictures
  • Collagenase
    9001-12-1
  • US $20.00-6.00 / kg
  • 0.99
  • Hebei Yanxi Chemical Co., Ltd.
COLLAGENASE TYPE X Collagenase from Clostridium histolyticum collagenase crude type ia cell culture*tested collagenase F. clostridium histolyticum collagenase from achromobacter iophagus collagenase sterile filtered type vii-S collagenase type I-S Matrix metalloprotease-1 Metallocollagenase Soycollagestin (VIAL) COLLAGENASE NB1 GMP GRADE COLLAGENASE NB4 FROM CL. HIST.STANDARD GRADE COLLAGENASE NB1 FROM CL. HIST.PREMIUM GRADE COLLAGENASE NB 8 FROM CL. HISTOLYTICUMBROAD RANGE COLLAGENASE NB6 FROM CL. HISTOLYTICUMGMP GRADE COLLAGENASE NB 5 FROM CL. HISTOLYTICUMSTERILE GRADE COLLAGENASE N REFERENCE CONTENT COLLAGENASE N REFERENCEIDENTITY COLLAGENASE NB 4G PROVED GRADE Collagenase from Clostridium histolyticum,Clostridiopeptidase A Collagenase,Type-III,from Clostridium histolyticum Collagenase V Collagenase from Clostridium histolyticum,lyophil. Recombinant Collagenase G >95% Recombinant Collagenase H >95% Recombinant Collagenase H/G >70% Recombinant Collagenase H/G >95% Collagenase NB 4G Proved Grade froM ClostridiuM histolyticuM crude Collagenase froM ClostridiuM histolyticuM(Purified) Anti-MMP1 (Cleaved-Phe100) antibody produced in rabbit collagenase type vii Collagenase, Paralithodes camtschatica Hepatopancreas Collagenase, Type II, Clostridium histolyticum Collagen-Peptide COLLAGENASE TYPE IA-S CELL CULTURE TESTED COLLAGENASE SIGMA BLEND TYPE N COLLAGENASE SIGMA BLEND TYPE L COLLAGENASE TYPE XI-S CELL CULTURE TESTED COLLAGENASE TYPE VII-S CELLCULTURE TESTE D COLLAGENASE TYPE III FRACTION A COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE IV COLLAGENASE F. CLOSTRIDIUM HISTOLYTICUM POWD.0.15-0.4 U/MG* COLLAGENASE FROM CLOSTRIDIUM HISTOLY-TIC UM, ~0.5-2 U/MG COLLAGENASE HIGH PURITY TYPE VII CELLCUL TURE TESTE COLLAGENASE SIGMA BLEND TYPE F COLLAGENASE SIGMA BLEND TYPE H COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE VIII COLLAGENASE TYPE IV-S COLLAGENASE FROM CLOSTRIDIUM HISTOLY-TIC UM, ~0.1 U/MG COLLAGENASE STERILE FILTERED TYPE XI-S COLLAGENACOLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE IA, FOR GENERAL USE COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE II COLLAGENASE CRUDE TYPE XI CELL CULTURETE STED COLLAGENASE TYPE S COLLAGENASE TYPE IA-S COLLAGENASE TYPE V-S CollagenaseFormIiExClostridiumHistolyt- CollagenaseFormIExClostridiumHistolyti-