Thiazolyl Blue
- CAS No.
- 298-93-1
- Chemical Name:
- Thiazolyl Blue
- Synonyms
- MTT;THIAZOLYL BLUE TETRAZOLIUM BROMIDE;METHYLTHIAZOLETETRAZOLIUM;METHYLTHIAZOLETETRAZOLIUM (MTT);MTT - CAS 298-93-1 - Calbiochem;THIAZOYL BLUE TETRAZOLIUM BROMIDE;METHYLTHIAZOLYLDIPHENYL-TETRAZOLIUM BROMIDE;3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE;2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2h-tetrazoliubromide;3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
- CBNumber:
- CB3383104
- Molecular Formula:
- C18H16BrN5S
- Molecular Weight:
- 414.32
- MDL Number:
- MFCD00011964
- MOL File:
- 298-93-1.mol
- MSDS File:
- SDS
| Melting point | 195 °C (dec.) (lit.) |
|---|---|
| Density | 1.4886 (estimate) |
| storage temp. | 2-8°C |
| solubility | methanol: 5 mg/mL, clear |
| form | powder |
| color | Yellow to orange |
| Water Solubility | Slightly soluble.Clear yellow solution at 5mg in 1ml of water on vortexing |
| Sensitive | Light Sensitive |
| λmax | 378 nm, 242 nm |
| BRN | 3825277 |
| Stability | Hygroscopic |
| Biological Applications | Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer |
| InChIKey | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
| CAS DataBase Reference | 298-93-1(CAS DataBase Reference) |
| EWG's Food Scores | 1 |
| FDA UNII | EUY85H477I |
| EPA Substance Registry System | 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (298-93-1) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS07,GHS08 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319-H335-H341 | |||||||||
| Precautionary statements | P201-P202-P261-P302+P352-P305+P351+P338-P308+P313 | |||||||||
| Hazard Codes | Xi,T,Xn | |||||||||
| Risk Statements | 46-68-36/37/38 | |||||||||
| Safety Statements | 22-24/25-53-45-36/37-26 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | XF8060000 | |||||||||
| F | 3-8-10 | |||||||||
| TSCA | Yes | |||||||||
| HS Code | 29341000 | |||||||||
| NFPA 704 |
|
Thiazolyl Blue price More Price(57)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 475989 | MTT - CAS 298-93-1 - Calbiochem Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. | 298-93-1 | 1g | $107 | 2024-03-01 | Buy |
| Sigma-Aldrich | 475989 | MTT - CAS 298-93-1 - Calbiochem Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. | 298-93-1 | 10g | $742 | 2024-03-01 | Buy |
| TCI Chemical | M3297 | MTT [for Biochemical Research] | 298-93-1 | 1G | $71 | 2024-03-01 | Buy |
| TCI Chemical | D0801 | 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide >98.0%(T) | 298-93-1 | 1g | $111 | 2024-03-01 | Buy |
| TCI Chemical | D0801 | 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide >98.0%(T) | 298-93-1 | 5g | $387 | 2024-03-01 | Buy |
Thiazolyl Blue Chemical Properties,Uses,Production
Chemical Properties
Thiazolyl Blue Tetrazolium Blue (MTT) is a water soluble tetrazolium salt yielding a yellowish solution when prepared in media or salt solutions lacking phenol red. Dissolved MTT is converted to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water insoluble formazan can be solubilized using isopropanol or other solvents and the dissolved material is measured spectrophotometrically yielding absorbance as a function of concentration of converted dye.
Uses
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Definition
ChEBI: Thiazolyl Blue is the bromide salt of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. It has a role as a dye and a colorimetric reagent. It contains a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium.
General Description
Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. It is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells. As an electron acceptor, it is utilized for studying nicotinamide adenine dinucleotide phosphate (NADP)-dependent dehydrogenases.
Biochem/physiol Actions
Cell permeable: yes
storage
+4°C
Purification Methods
It is recrystallised by dissolving in MeOH containing a few drops of HBr and then adding dry Et2O to complete the crystallisation, wash the needles with Et2O and dry them in a vacuum desiccator over KOH. [Beyer & Pyl Chem Ber 87 1505 1954, Beilstein 27 III/IV 6045.]
Thiazolyl Blue Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Suzhou Laing Biotechnology Co.,Ltd | +86-13004516015 +86-18149392822 | christina@laingbiotec.com | China | 778 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21695 | 55 |
| Nanjing ChemLin Chemical Industry Co., Ltd. | 025-83697070 | product@chemlin.com.cn | CHINA | 3012 | 60 |
| career henan chemical co | +86-0371-86658258 | sales@coreychem.com | China | 29914 | 58 |
| Shandong chuangyingchemical Co., Ltd. | 18853181302 | sale@chuangyingchem.com | CHINA | 5909 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-61398051 +8613650506873 | sales@chemdad.com | China | 39916 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 47465 | 58 |
| Shaanxi Dideu Medichem Co. Ltd | +86-29-87569266 15319487004 | 1015@dideu.com | China | 2263 | 58 |
| Zhengzhou Alfa Chemical Co.,Ltd | +8618530059196 | sale04@alfachem.cn | China | 12477 | 58 |
| SINOPRO.CO.LTD | 0082-42-721-7177 | michael@sinopro.co.kr | South Korea | 298 | 58 |
View Lastest Price from Thiazolyl Blue manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
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2023-02-07 | Thiazolyl Blue
298-93-1
|
US $0.00 / G | 100G | 98%min | 30kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | |
 |
2022-02-21 | 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide
298-93-1
|
US $0.00 / KG | 1KG | 99.3% | 100 tons | Honest Joy Holdings Limited
|
|
 |
2021-07-13 | Thiazolyl Blue
298-93-1
|
US $15.00-10.00 / KG | 1KG | 99%+ HPLC | Monthly supply of 1 ton | Zhuozhou Wenxi import and Export Co., Ltd |
-
- Thiazolyl Blue
298-93-1
- US $0.00 / G
- 98%min
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide
298-93-1
- US $0.00 / KG
- 99.3%
- Honest Joy Holdings Limited
-
- Thiazolyl Blue
298-93-1
- US $15.00-10.00 / KG
- 99%+ HPLC
- Zhuozhou Wenxi import and Export Co., Ltd