Ribonuclease A
| Melting point | 243 °C (dec.) |
|---|---|
| alpha | D25 (per mg N) -0.47° (c = 5) |
| storage temp. | -20°C |
| solubility | Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride |
| form | solid |
| color | white |
| PH | pH : 8.0 |
| Water Solubility | Soluble in water. |
| Sensitive | Moisture Sensitive |
| EWG's Food Scores | 1 |
| EPA Substance Registry System | Nuclease, ribo- (9001-99-4) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261-P284-P501 |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| RTECS | RF0760000 |
| F | 10-21 |
| TSCA | Yes |
| HS Code | 35079090 |
Ribonuclease A price More Price(67)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | RNASEA-RO | RNase A from bovine pancreas | 9001-99-4 | 25mg | $59.5 | 2024-03-01 | Buy |
| Sigma-Aldrich | RNASEA-RO | RNase A from bovine pancreas | 9001-99-4 | 100mg | $126 | 2024-03-01 | Buy |
| Sigma-Aldrich | R7884 | Ribonuclease B from bovine pancreas BioReagent,≥50?Kunitzunits/mgprotein,≥80%(SDS-PAGE) | 9001-99-4 | 500μg | $52.6 | 2024-03-01 | Buy |
| Sigma-Aldrich | R1153 | RNase B Glycoprotein Standard from bovine pancreas Proteomics Grade | 9001-99-4 | 500μg | $351 | 2024-03-01 | Buy |
| Sigma-Aldrich | 55674-M | RNaseA, Bovine Pancreas | 9001-99-4 | 55674-50KU | $377 | 2024-03-01 | Buy |
Ribonuclease A Chemical Properties,Uses,Production
Chemical Properties
Lyophilized
Uses
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.
Uses
Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas.
Uses
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.
General Description
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.
Biochem/physiol Actions
Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
Purification Methods
Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins.
Ribonuclease A Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Jiangsu Boquan Biotechnology Co., Ltd. | +86-18168774353 | boquanshengwu003@boquansw.com | China | 248 | 58 |
| Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 | info@dakenam.com | China | 15928 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21695 | 55 |
| career henan chemical co | +86-0371-86658258 | sales@coreychem.com | China | 29914 | 58 |
| Hubei xin bonus chemical co. LTD | 86-13657291602 | linda@hubeijusheng.com | CHINA | 22968 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-61398051 +8613650506873 | sales@chemdad.com | China | 39916 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 47465 | 58 |
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| Hubei Ipure Biology Co., Ltd | +8613367258412 | ada@ipurechemical.com | China | 10326 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | 0551-65418671 | sales@tnjchem.com | China | 34572 | 58 |
View Lastest Price from Ribonuclease A manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2023-07-10 | Ribonuclease A
9001-99-4
|
US $88.00-81.00 / g | 5g | 99% | 10KG | Jiangsu Boquan Biotechnology Co., Ltd. | |
 |
2022-12-24 | Ribonuclease A
9001-99-4
|
US $9.90 / kg | 1kg | 99% | 1000kg | Hebei Duling International Trade Co. LTD | |
 |
2022-09-22 | Ribonuclease A (bovine pancreas)
9001-99-4
|
US $0.00 / kg | 1kg | 99% | 1000kg | henan kanbei chemical co.,ltd |
-
- Ribonuclease A
9001-99-4
- US $88.00-81.00 / g
- 99%
- Jiangsu Boquan Biotechnology Co., Ltd.
-
- Ribonuclease A
9001-99-4
- US $9.90 / kg
- 99%
- Hebei Duling International Trade Co. LTD
-
- Ribonuclease A (bovine pancreas)
9001-99-4
- US $0.00 / kg
- 99%
- henan kanbei chemical co.,ltd
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