ChemicalBook >> CAS DataBase List >>REVERSE TRANSCRIPTASE

REVERSE TRANSCRIPTASE

CAS No.
9068-38-6
Chemical Name:
REVERSE TRANSCRIPTASE
Synonyms
HIV-1 RT;abs60073;revertase;REVERSCRIPT 1;REVERSE TRANSCRIPTASE;HIV REVERSE TRANSCRIPTASE;AMV REVERSE TRANSCRIPTASE;REVERSE TRANSCRIPTASE AMV;RTL reverse transcriptase;M-MLV REVERSE TRANSCRIPTASE
CBNumber:
CB4702207
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD00283754
MOL File:
Mol file
Last updated:2023-04-23 13:52:06

REVERSE TRANSCRIPTASE Properties

storage temp. -20°C
form Liquid
color colorless

SAFETY

Risk and Safety Statements

WGK Germany  3
HS Code  3504009000

REVERSE TRANSCRIPTASE price More Price(4)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich M1302 M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis 9068-38-6 40000units $397 2024-03-01 Buy
Alfa Aesar J60167 Reverse Transcriptase, murine, Moloney Murine Leukemia Virus 9068-38-6 10kilounit $123 2021-12-16 Buy
Usbiological R1992-08 Reverse Transcriptase 9068-38-6 100ug $460 2021-12-16 Buy
American Custom Chemicals Corporation ENZ0000287 M-MLV REVERSE TRANSCRIPTASE 95.00% 9068-38-6 5MG $499.05 2021-12-16 Buy
Product number Packaging Price Buy
M1302 40000units $397 Buy
J60167 10kilounit $123 Buy
R1992-08 100ug $460 Buy
ENZ0000287 5MG $499.05 Buy

REVERSE TRANSCRIPTASE Chemical Properties,Uses,Production

Description

Enzyme possessed by retroviruses that allows transcription of DNA from an RNA template. Such an enzyme has never been isolated from other sources, but speculation about its possible occurrence continues. Such an enzyme is widely used experimentally in genetic engineering to allow the formation of complementary DNA (cDNA) from purified RNA. The discovery of this enzyme faulted the widely held central dogma, which stated that the information flow in nature was always from DNA to RNA.

Uses

Synthesizes a complementary DNA strand using single-stranded RNA or DNA in the presence of a primer

Uses

Reverse Transcriptase AMV is suitable for:

  • First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
  • Dideoxy DNA sequencing
  • Primer extension
  • RNA sequencing
  • 3′-end labeling of DNA fragments
  • Generation of single-stranded probes for genomic footprint experiments

Uses

M-MLV Reverse Transcriptase has been used:

  • for the preparation of cDNA libraries or for first strand cDNA synthesis
  • for use in a 2-step RT-PCR assay
  • for the synthesis of cDNA that is further used in cloning

General Description

M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Purification Methods

This enzyme produces the complementary DNA from the RNA (as template). These are purified by solubilising the virus with non-ionic detergents. Lysed virions are adsorbed on DEAE-cellulose or DEAE-Sephadex columns, and the enzymes are eluted with a salt gradient, then chromatographed on a phosphocellulose column and fractions with enzyme activity are eluted in a salt gradient. They are also purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 1977, Smith Methods Enzymol 65 560 1980; see commercial catalogues for other transcriptases.]

REVERSE TRANSCRIPTASE Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 129)Suppliers
Supplier Tel Email Country ProdList Advantage
Shanghai Daken Advanced Materials Co.,Ltd
+86-371-66670886 info@dakenam.com China 14936 58
career henan chemical co
+86-0371-86658258 15093356674; factory@coreychem.com China 29826 58
Creative Enzymes
1-516-855-7709 info@creative-enzymes.com United States 8748 58
Hebei Duling International Trade Co. LTD
+8618032673083 sales05@hbduling.cn China 15755 58
Nanjing Duly Biotech Co.,Ltd
+undefined18013301590 melody@njduly.com China 2452 58
J & K SCIENTIFIC LTD. 010-82848833 400-666-7788 jkinfo@jkchemical.com China 96815 76
BEST-REAGENT 400-1166-196 18981987031 cdhxsj@163.com China 11726 57
Chengdu Ai Keda Chemical Technology Co., Ltd. 4008-755-333 18080918076 800078821@qq.com China 9725 55
Nanjing Sunlida Biological Technology Co., Ltd. 025-57798810 sales@sunlidabio.com China 3750 55
Shanghai BeiZhuo Biotech Co., Ltd. 021-61119791,13386096464 bzswkf@foxmail.com China 3924 50

View Lastest Price from REVERSE TRANSCRIPTASE manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ pictures 2020-01-10 REVERSE TRANSCRIPTASE, M-MLV, RNASEH+
9068-38-6
US $1.00 / KG 1KG 99% 200kg Career Henan Chemical Co
M-MLV REVERSE TRANSCRIPTASE MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE Nucleotidyltransferase, deoxyribonucleate, RNA-dependent REVERSE TRANSCRIPTASE, FOR MOL. BIOLOGY, DRY ICE, 500 U* REVERSE TRANSCRIPTASE FROM AVIAN*MYELOBL ASTOSIS VIR CYSCRIBE REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE, REC., INDUSTRIAL GMP GRADE amv-reverse transcriptase from avian myeloblastosis virus revertase Reverse Trancscriptase, Molony Mouse Leukemia Virus, Recombinant AVIAN MYELOBLASTOSIS VIRUS REVERSE TRANSCRIPTASE HIV REVERSE TRANSCRIPTASE HIV REVERSE TRANSCRIPTASE, RECOMBINANT, E COLI HIV-1 REVERSE TRANSCRIPTASE HIV-1 RT AMV REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE (HC) RNA-DIRECTED DNA POLYMERASE REVERSCRIPT 1 REVERSE TRANSCRIPTASE REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE, AMV, (HC) REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ M-MLV REVERSE TRANSCRIPTASE, RNASE H MINUS M-MLV REVERSE TRANSCRIPTASE, RNASE H MINUS, POINT MUTANT Revertase, RNA-directed DNA polymerase Reverse Transcriptase from HIV, Recombinant Native Avian myeloblastosis virus AMV-Reverse Transcriptase M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis ToloScript Reverse Transcriptase abs60073 M-MLV Reverse transcriptase BR RTL reverse transcriptase 9068-38-6 Nucleic Acid Amplification Molecular Biology PCR/Amplification Plant Biotechnology Plant Molecular Biology RT-PCR BioChemical Enzyme 2.7.x.x Phosphorus containing groupsMore...Close... AmplificationMolecular Biology cDNA LabelingApplication Index PCRPlant Molecular Biology RT-PCREnzyme Class Index 2.x.x.x Transferases Functional Profiling of Stem Cells Gene Expression&Analysis Molecular Biology Tested Nucleic Acid Amplification PCR/Amplification RT-PCRMolecular Biology Stem Cell Biology