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Benzidine

CAS No.
92-87-5
Chemical Name:
Benzidine
Synonyms
BENZIDINE-D8;Benzidin;Benzidina;benzidine solution;BENZIDINE-RINGS-D8;biphenyl-4,4’-diamine;4,4’-biphenylenediamine;BENZIDINE-D8 (RINGS-D8);ai3-00140;Benzydyna
CBNumber:
CB5152058
Molecular Formula:
C12H12N2
Molecular Weight:
184.24
MDL Number:
MFCD00039765
MOL File:
92-87-5.mol
MSDS File:
SDS
Last updated:2024-03-14 15:18:26

Benzidine Properties

Melting point 127-128 °C
Boiling point 402°C
Density 1.25
vapor pressure Based on the specific vapor density value of 6.36 (Sims et al., 1988), the vapor pressure wascalculated to be 0.83 at 20 °C.
refractive index 1.6266 (estimate)
Flash point 11 °C
storage temp. 2-8°C
solubility Soluble in ethanol (U.S. EPA, 1985) and ether (1 g/50 mL) (Windholz et al., 1983)
pka 4.66(at 30℃)
color Grayish-yellow, crystalline powder; white or sltlyreddish crystals, powder
Water Solubility Sparingly soluble. <0.1 g/100 mL at 22 ºC
Merck 13,1077
BRN 742770
Henry's Law Constant (x 10-11 atm?m3/mol): 3.88 at 25 °C (estimated, Howard, 1989)
Exposure limits Because it is a carcinogen and readily absorbed through skin, no TLV has been assigned. Exposure should be at an absolute minimum.
Recognized Human Carcinogen (ACGIH); Human Carcinogen (MSHA); Carcinogen (OSHA); Human Sufficient Evidence, Animal Sufficient Evidence (IARC).
Stability Stable. Combustible. Incompatible with strong oxidizing agents.
InChIKey HFACYLZERDEVSX-UHFFFAOYSA-N
CAS DataBase Reference 92-87-5(CAS DataBase Reference)
EWG's Food Scores 8-10
FDA UNII 2X02101HVF
Proposition 65 List Benzidine (and its salts)
NIST Chemistry Reference Benzidine(92-87-5)
IARC 1 (Vol. 29, Sup 7, 99, 100F) 2012
EPA Substance Registry System Benzidine (92-87-5)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictogramsGHS hazard pictograms
GHS07,GHS08,GHS09
Signal word  Danger
Hazard statements  H302-H350-H410
Precautionary statements  P201-P202-P264-P273-P301+P312-P308+P313
Hazard Codes  T,N,F,Xn
Risk Statements  45-22-50/53-52/53-39/23/24/25-23/24/25-11-36/37/38-20/21/22-51/53-67
Safety Statements  53-45-60-61-36/37-16-7-36-26
RIDADR  UN 1885 6.1/PG 2
WGK Germany  3
RTECS  DC9625000
8
HazardClass  6.1(a)
PackingGroup  II
HS Code  29215900
Toxicity Acute oral LD50 for mice 214 mg/kg, rats 309 mg/kg (quoted, RTECS, 1985).

Benzidine price More Price(30)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 40005 Benzidine solution certified reference material, 5000?μg/mL in methanol 92-87-5 1mL $62.3 2024-03-01 Buy
Sigma-Aldrich 12115 Benzidine purum p.a., ≥98.0% (N) 92-87-5 10g $486 2024-03-01 Buy
Sigma-Aldrich 12115 Benzidine purum p.a., ≥98.0% (N) 92-87-5 50g $1580 2022-05-15 Buy
Sigma-Aldrich B3503 Benzidine ≥98.0% (N) 92-87-5 10g $693 2024-03-01 Buy
Sigma-Aldrich 31614 Benzidine analytical standard 92-87-5 100mg $68.5 2024-03-01 Buy
Product number Packaging Price Buy
40005 1mL $62.3 Buy
12115 10g $486 Buy
12115 50g $1580 Buy
B3503 10g $693 Buy
31614 100mg $68.5 Buy

Benzidine Chemical Properties,Uses,Production

Chemical properties

It appears as white or light pink crystalline powder with a melting point of 125 ℃, the boiling point of 400 ℃, (98.7kPa) and the relative density of 1.250 (20/4 ℃). It is soluble in boiling ethanol, acetic acid and diluted hydrochloric acid, slightly soluble in ether, slightly soluble in boiling water and slightly soluble in cold water. Its color is darkened under air and light. Analysis reagents are usually benzodine hydrochloride or acetate, which is more soluble, and sulfate is commonly used in the industry. Benzidine acetate is white or nearly white crystals, being soluble in water, acetic acid and hydrochloric acid, as an indicator. Benzidine sulfate is a white crystalline powder or flaky crystal, being soluble in ether, very slightly soluble in water, dilute acid and alcohol.

Uses

Important dye intermediates, benzidine and its derivatives can be used to make direct dyes, acid dyes, vat dyes, ice dyed dyes, sulfur dyes, reactive dyes and organic pigments. More than 250 dyes are made from benzidine, of which the most important is the direct black EW. The benzidine yellow is a widely used organic pigment.

Toxicity

Benzidine is highly toxic, can be absorbed through the respiratory tract, skin and digestive tract, and is highly toxic, belonging to a carcinogen. Both solid and vapor are quickly absorbed through the skin, causing blood damage and causing bladder cancer. Mistakenly eating it can cause nausea, vomiting, liver and kidney damage. Mice oral LD50: 214mg / kg (body weight), rat oral LD50: 309 mg / kg (body weight). Rabbits and dogs have an oral minimum lethal dose of 200mg / kg (weight). The major toxic effect is hemorrhagic cystitis. The effect on the formation of methemoglobin is weak. It has stimulation effect on the skin and mucous membranes, being capable of causing contact dermatitis. It can cause liver cancer in mice and hamsters, causing rat liver, Zymbal gland, breast and colon cancer and cause bladder cancer in dogs. A variety of short-term mutagenicity test has given positive results. The International Agency for Research on Cancer (IARC) classifies it as a human carcinogen (well-documented) with the targets being bladder. The relative risk of bladder cancer in dyes chemical worker is 19 with the incubation period of about 19 years.

Description

Benzidine is a white, greyish-yellow, or slightly reddish crystalline solid or powder. The major use for benzidine is in the production of dyes, especially azo dyes in the leather, textile, and paper industries and as a synthetic precursor in the preparation and manufacture of dyestuffs. It is also used in the manufacture of rubber, as a reagent, and as a stain in microscopy. It is slightly soluble and slowly changes from a solid to a gas.

Chemical Properties

Benzidine is a white, grayish-yellow, or slightly reddish crystalline solid or powder. The major use for benzidine is in the production of dyes, especially azo dyes in the leather, textile, and paper industries, as a synthetic precursor in the preparation and manufacture of dyestuffs. It is also used in the manufacture of dyes and rubber, as a reagent, and as a stain in microscopy. It is slightly soluble and slowly changes from a solid to a gas.

Chemical Properties

Off-White Solid

Chemical Properties

Benzidine is a white, grayish-yellow crystalline solid or powder. Turns brownish-red on exposure to air and light;

Physical properties

Grayish-yellow to pale reddish powder or crystals. Darkens on exposure to air or light. Odorless.

Uses

Benzidine was used extensively in the manu facture of dyes. Because of its cancer-causingeffects in humans, its application in dyes hasbeen curtailed. Other uses of this compoundare in chemical analysis: as a reagent for thedetermination of hydrogen peroxide in milkand in the analysis of nicotine. Its hydrochlo ride is used as a reagent to analyze metalsand sulfate.

Uses

Manufacture of dyestuffs; hardener for rubber; laboratory reagent

Uses

Potentially mutagenic compound.

Preparation

1-Nitrobenzene restore 1,2-Diphenylhydrazine?turn with acid rearrangement.

Production Methods

Benzidine production is now exclusively for captive consumption and must be carried out in closed systems under stringent workplace controls. Benzidine is used in the synthesisofdyesanddyeintermediates,asahardenerforrubber, and as a laboratory reagent. The ?rst successful synthetic direct dye was Congo Red, a diazo derivative prepared from benzidinebyBoettigerin1884.Nearlyalldirectdyesareazo products. Congo Red is used in humans intravenously for the medical diagnosis of amyloidosis. The basis for its use is an unexplained af?nity for amyloid, which rapidly removes the dye from the blood. It is used medically for the management of profuse capillary hemorrhage such as the one occurring in septicemias and in the terminal phases of leukemia.

Definition

ChEBI: A member of the class of biphenyls that is 1,1'-biphenyl in which the hydrogen at the para-position of each phenyl group has been replaced by an amino group.

Synthesis Reference(s)

The Journal of Organic Chemistry, 41, p. 2661, 1976 DOI: 10.1021/jo00877a041
Synthesis, p. 40, 1976 DOI: 10.1055/s-1976-23952

General Description

A grayish-yellow to grayish-red, crystalline solid. Toxic by ingestion, inhalation, and skin absorption. Combustion produces toxic oxides of nitrogen. Used to make other chemicals and in chemical and biological analysis.

Air & Water Reactions

Darkens on exposure to air and light. Soluble in hot water.

Reactivity Profile

Benzidine forms insoluble salts with sulfuric acid. Can be diazotized, acetylated and alkylated. Is hypergolic with red fuming nitric acid . Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.

Hazard

Highly toxic by ingestion, inhalation, and skin absorption. Confirmed carcinogen.

Health Hazard

Exposure to benzidine causes irritation to the eyes. Laboratory animals exposed to benzidine at as low as 0.01% to 0.08% in food showed adverse health effects, such as organ weight decrease in the liver, kidney, and body weight, and an increase in spleen weight, swelling of the liver, and blood in the urine. Exposure may cause an increase in urination, blood in the urine, and urinary tract tumors. Benzidine is considered acutely toxic to humans by ingestion, with an estimated oral lethal dose of between 50 and 500 mg/kg. The symptoms of acute ingestion exposure include cyanosis, headache, mental confusion, nausea, and vertigo. Dermal exposure may cause skin rashes and irritation. Prolonged exposure to benzidine causes bladder injury in humans

Health Hazard

Benzidine is a known carcinogen, causingbladder cancer in humans. Numerous reportsin the literature document its carcinogenicityin animals and humans. Oral or subcutaneousapplication of this compound in experimentalanimals produced tumors in liver, blood,lungs, and skin. The routes of entry intohuman body are primarily the inhalation of its dusts and absorption through skin. Whilehumans and dogs develop bladder cancerfrom benzidine, rodents primarily developliver cancer.Relatively little information is availableon the noncancer health hazard from ben zidine. The acute oral toxicity in animalswas moderate. Ingestion can produce nausea,vomiting, kidney, and liver damage. The oralLD50 values in test animals were in the range150–300 mg/kg.The mechanism of carcinogenicity of ben zidine is thought to involve its metabolictransformations forming reactive intermedi ates binding to DNA. Such DNA adductshave been identified in rodent liver. It testedpositive in most genotoxic tests. Its car cinogenicity may possibly be related to theslow rate of liver detoxification by acetyla tion allowing activation of benzidine or itsmetabolites in urine (Whysner et al. 1996)..

Health Hazard

Poisonous if inhaled, swallowed or absorbed through skin. May cause contact dermatitis, irritation or sensitization. Ingestion may cause nausea and vomiting.

Safety Profile

Confirmed human carcinogen producing bladder tumors. Experimental carcinogenic and tumorigenic data. Poison by ingestion and intraperitoneal routes. Human mutation data reported. Can cause damage to blood, including hemolysis and bone marrow depression. On ingestion causes nausea and vomiting, which may be followed by liver and kidney damage. Any exposure is considered extremely hazardous. When heated to decomposition it emits highly toxic fumes of NOx. See also AROMATIC AMINES.

Potential Exposure

Benzidine is used primarily in the manufacture of azo dyestuffs; there are over 250 of these produced. Other uses, including some which may have been discontinued, are in the rubber industry as a hardener; in the manufacture of plastic films; for detection of occult blood in feces, urine, and body fluids; in the detection of H2O2 in milk; in the production of security paper; and as a laboratory reagent in determining HCN, sulfate, nicotine, and certain sugars. No substitute has been found for its use in dyes. Free benzidine is present in the benzidine-derived azo dyes. According to industry, quality control specifications require that the level not exceed 20 ppm and in practice the level is usually below 10 ppm. Regulations in the USA concerning this chemical define strict procedures to avoid worker contact: mixture containing 0.1% or more must be maintained in isolated or closed systems; employees must observe special personal hygiene rules, and certain procedures must be followed in case of emergencies. Some p-phenylenediamine compounds have been used as rubber components, and DFG warns of danger of skin sensitization. Benzidine and dyes metabolized to benzidine: The following three benzidine-based dyes have been tested and found to cause cancer in rodents after oral exposure for 13 weeks (NCI 1978, IARC 1982): C.I. direct black 38 (CAS 1937-37-7) caused liver cancer in rats and mice, mammary-gland cancer in mice, and colon and urinary-bladder cancer in rats. C.I. direct Blue 6 (CAS 2602-46-2) caused liver cancer in rats. C.I. direct brown 95 (CAS 16071-86-6) caused hepatocellular adenoma in the liver and one malignant liver tumor in rats.

First aid

If this chemical gets into the eyes, remove anycontact lenses at once and irrigate immediately for at least15 min, occasionally lifting upper and lower lids. Seek medical attention immediately. If this chemical contacts theskin, remove contaminated clothing and wash immediatelywith soap and water. Seek medical attention immediately. Ifthis chemical has been inhaled, remove from exposure,begin rescue breathing (using universal precautions, including resuscitation mask) if breathing has stopped and CPR ifheart action has stopped. Transfer promptly to a medicalfacility. When this chemical has been swallowed, get medical attention. Use gastric lavage if ingested followed bysaline catharsis. Medical observation is recommended for24-48 h after breathing overexposure, as pulmonary edemamay be delayed. As first aid for pulmonary edema, a doctoror authorized paramedic may consider administering a corticosteroid spray.

Carcinogenicity

Benzidine is known to be a human carcinogen based on sufficient evidence of carcinogenicity from studies in humans.

Source

Benzidine can enter the environment by transport, use, and disposal, or by dyes and pigments containing the compound. A photodegradation product of 3,3′-dichlorobenzidine.
Based on laboratory analysis of 7 coal tar samples, benzidine was ND (EPRI, 1990).

Environmental Fate

Biological. In activated sludge, <0.1% mineralized to carbon dioxide after 5 d (Freitag et al., 1985). Kincannon and Lin (1985) reported a half-life of 76 d when benzidine in sludge was applied to a sandy loam soil.
Soil. Benzidine was added to different soils and incubated in the dark at 23 °C under a carbon dioxide-free atmosphere. After 1 yr, 8.3 to 11.6% of the added benzidine degraded to carbon dioxide primarily by microbial metabolism and partially by hydrolysis (Graveel et al., 1986). Tentatively identified biooxidation compounds using GC/MS include hydroxybenzidine, 3- hydroxybenzidine, 4-amino-4′-nitrobiphenyl, N,N′-dihydroxybenzidine, 3,3′-dihydroxybenzidine and 4,4′-dinitrobiphenyl (Baird et al., 1977). Under aerobic conditions, the half-life was estimated to be 2 to 8 d (Lu et al., 1977).
Chemical/Physical. Benzidine is not subject to hydrolysis (Kollig, 1993). Reacts with HCl forming a salt (C12H12N2?2HCl) that is very soluble in water (61.7 mg/L at 25 °C) (Bowman et al., 1976).

storage

Benzidine should be kept stored in a cool, well-ventilated area, in closed, sealed containers and out of sunlight and away from heat.

Shipping

UN1885 Benzidine, Hazard Class: 6.1; Labels: 6.1—Poisonous materials. PGII.

Properties and Applications

white or pink micro crystalline powder. Melting point 125 ℃, boiling point 400 ℃, relative density 1.250 (20 ℃). Soluble in ethanol, rare hydrochloric acid and acetic acid boiling, slightly soluble in ethyl ether, slightly soluble in water, very slightly soluble in cold water. In the air and light color line darker. This product is the dye and organic pigments intermediate.

Purification Methods

Its solution in *benzene is decolorized by percolating through two 2-cm columns of activated alumina, then concentrated until benzidine crystallises on cooling. Recrystallise alternately from EtOH and *benzene to constant absorption spectrum [Carlin et al. J Am Chem Soc 73 1002 1951]. It has also been crystallised from hot water (charcoal) and from diethyl ether. Dry it under vacuum in an Abderhalden pistol. Store it in the dark in a stoppered container. CARCINOGENIC. [Beilstein 13 IV 364.]

Toxicity evaluation

Industries release benzidine into the environment in the form of liquid waste and sludges. Benzidine may also be released into the environment due to spillage during transport. In air, benzidine is found bound to suspended particles or as a vapor, which may be brought back to the earth’s surface by rain or gravity.

Incompatibilities

Dust may form explosive mixture with air. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. On contact with strong reducing agents, such as hydrides may form flammable gases. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides. Contact with red fuming nitric acid may cause fire. Oxidizes in air. Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides.

Waste Disposal

Incineration; oxides of nitrogen are removed from the effluent gas by scrubber, catalytic or thermal device. Package spill residues and sorbent media in 17 hour epoxy-lined drums and move to an EPA-approved disposal site. Treatment may include destruction by potassium permanganate oxidation, hightemperature incineration, or microwave plasma methods. 398 Benzidine Encapsulation by organic polyester resin or silicate fixation. These disposal procedures should be confirmed with responsible environmental engineering and regulatory officials.

Precautions

At high temperatures, benzidine breaks down and releases highly poisonous fumes. During use and handling, workers should wear butyl rubber gloves, goggles, and full body plastic coveralls and ensure that no skin is exposed.

Global( 192)Suppliers
Supplier Tel Email Country ProdList Advantage
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21700 55
ATK CHEMICAL COMPANY LIMITED
+undefined-21-51877795 ivan@atkchemical.com China 32480 60
career henan chemical co
+86-0371-86658258 sales@coreychem.com China 29914 58
Jilin Chinese Academy of Sciences - Yanshen Technology Co., Ltd.
0431-80514535 13634302652 Extension@chemextension.com CHINA 967 58
Hubei Jusheng Technology Co.,Ltd.
18871490254 linda@hubeijusheng.com CHINA 28180 58
Hubei xin bonus chemical co. LTD
86-13657291602 linda@hubeijusheng.com CHINA 22968 58
Chongqing Chemdad Co., Ltd
+86-023-61398051 +8613650506873 sales@chemdad.com China 39916 58
Alchem Pharmtech,Inc.
8485655694 sales@alchempharmtech.com United States 63711 58
Richest Group Ltd
18017061086 oled@richest-group.com CHINA 5601 58
Shanghai Daeyeon Chemicals Co., Ltd
021-64478606 +8615900664856 daeyeon001@vip.163.com China 2062 58

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View Lastest Price from Benzidine manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Benzidine pictures 2021-07-13 Benzidine
92-87-5
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
Benzidine pictures 2021-07-10 Benzidine
92-87-5
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
Benzidine pictures 2021-07-02 Benzidine
92-87-5
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
  • Benzidine pictures
  • Benzidine
    92-87-5
  • US $15.00-10.00 / KG
  • 99%+ HPLC
  • Zhuozhou Wenxi import and Export Co., Ltd
  • Benzidine pictures
  • Benzidine
    92-87-5
  • US $15.00-10.00 / KG
  • 99%+ HPLC
  • Zhuozhou Wenxi import and Export Co., Ltd
  • Benzidine pictures
  • Benzidine
    92-87-5
  • US $15.00-10.00 / KG
  • 99%+ HPLC
  • Zhuozhou Wenxi import and Export Co., Ltd
[1,1'-Biphenyl]-4,4'-diamine 4,4’-bianiline 4,4’-diamino-bipheny 4,4’-diaminodiphenyl benzydyna(polish) biphenyl -4,4'-ylenediamine Biphenyl,4,4'-diamino- Fast Corinth Base B fastcorinthbaseb NCI-C03361 p,p’-bianiline p,p’-Bianiline,[1,1’-Biphenyl]-4,4’-diamine p,p’-Diaminodiphenyl p,p’-dianiline p,p'-Bianiline p,p-Bianiline p,p'-Dianiline benzidine 1,1'-biphenyl-4,4'-diamine 4,4'-diaminobiphenyl biphenyl-4,4'-ylenediamine Benzidine Standard Benzidine,4,4′-Diaminobiphenyl Benzidine 250mg [92-87-5] NSC 146476 4.4'-Benzidi 4,4’-diphenylenediamine 4,4'-Bianiline 4,4'-Biphenylenediamine 4,4'-Diaminodiphenyl 4,4'-Diphenylenediamine 4'-Amino[1,1'-biphenyl]-4-ylamine ai3-00140 benzidin(czech) benzidinebase Benzydyna p-benzidine p-Diaminodiphenyl Rcra waste number U021 rcrawastenumberu021 P,P'-DIAMINOBIPHENYL SPECS AC-907/25014310 4,4'-Diaminobiphenyl Solution, 100ppm (1,1'-Biphenyl)-4,4'-di 4-(4-aminophenyl)benzenamine BENZIDINE, 1X1ML, CH2CL2, 500UG/ML BENZIDINE, 100MG, NEAT BENZIDINE FREE BASE BENZIDINE, 1X1ML, MEOH, 5000UG/ML BENZIDINE OEKANAL, 100 MG BENZIDINE FREE BASE ISOPAC BENZIDINE 99.5% BENZIDINE (RING-D8) BENZIDINE c.i. azoic diazo component 112 C.I. 37225 4,4'-DIAMINOBIPHENYL 4,4'-DIAMINO(BIPHENYL-D8) 4,4'-diamino-1,1'-biphenyl 4,4'-BENZIDINE 4,4'-Biphenyldiamine