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Tetramethylbenzidine

Tetramethylbenzidine Structure
Tetramethylbenzidine structure
CAS No.
54827-17-7
Chemical Name:
Tetramethylbenzidine
Synonyms
TMB;Tmbz;TMB SUBSTRATE;Tetramethylbenzidine (TMB);3,3,5,5-TETRAMETHYLBENZIDINE, STANDARD F OR GC;4-(4-Amino-3,5-dimethylphenyl)-2,6-dimethylaniline;TMB/M;TMB-E;TMB/H;TMB-B
CBNumber:
CB6682185
Molecular Formula:
C16H20N2
Molecular Weight:
240.34
MDL Number:
MFCD00007748
MOL File:
54827-17-7.mol
MSDS File:
SDS
Last updated:2024-04-17 18:50:11
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Tetramethylbenzidine Properties

Melting point 168-171 °C(lit.)
Boiling point 100 °C
Density 1
refractive index 1.5519 (estimate)
storage temp. 2-8°C
solubility Slightly soluble. <0.1 g/100 mL at 20°C.
pka 4.49±0.10(Predicted)
form tablet
color Cream
Water Solubility Slightly soluble. <0.1 g/100 mL at 20 ºC
Sensitive Light Sensitive
BRN 2808541
Stability Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
CAS DataBase Reference 54827-17-7(CAS DataBase Reference)
FDA UNII 3B3T5CB8EO
EPA Substance Registry System 3,3',5,5'-Tetramethylbenzidine (54827-17-7)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictogramsGHS hazard pictograms
GHS07,GHS08,GHS02
Signal word  Warning
Hazard statements  H302-H315-H319-H335-H225-H312-H332-H370
Precautionary statements  P210-P280-P303+P361+P353-P261-P280a-P304+P340-P305+P351+P338-P405-P501a-P260-P280h-P308+P311a
Hazard Codes  Xi,Xn
Risk Statements  36/38-36/37/38-22-41
Safety Statements  26-36-36/37-39
RIDADR  UN 2796 8/PG 2
WGK Germany  3
RTECS  DV2300000
F  8-10
TSCA  Yes
HazardClass  6.1
HS Code  29215990
NFPA 704
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Tetramethylbenzidine price More Price(69)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich T5525 3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet 54827-17-7 50tablets $223 2024-03-01 Buy
Sigma-Aldrich T5525 3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet 54827-17-7 100tablets $381 2024-03-01 Buy
Sigma-Aldrich 11484281001 BM Blue POD Substrate, soluble 3,3′-5,5′-Tetramethylbenzidine (TMB) 54827-17-7 100mL $146 2024-03-01 Buy
Sigma-Aldrich 1.08622 3,3′,5,5′-Tetramethylbenzidine GR for analysis 54827-17-7 1g $145 2024-03-01 Buy
Sigma-Aldrich 09743 3,3′,5,5′-Tetramethylbenzidine standard for GC 54827-17-7 250mg $36.9 2024-03-01 Buy
Product number Packaging Price Buy
T5525 50tablets $223 Buy
T5525 100tablets $381 Buy
11484281001 100mL $146 Buy
1.08622 1g $145 Buy
09743 250mg $36.9 Buy

Tetramethylbenzidine Chemical Properties,Uses,Production

Description

Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.

Chemical Properties

Tetramethylbenzidine is a white or light yellow solid, odorless, tasteless, insoluble in water, easily soluble in acetone, ether, dimethyl sulfoxide, dimethylformamide and other organic solvents. It forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights.

Uses

3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.

Preparation

The synthesis of 3,3',5,5'-Tetramethylbenzidine involved using 2,6-dimethylaniline as the raw material. The process included activation, oxidative coupling, and purification, resulting in the production of pure material. The yield of 3,3,5,5-tetramethylbenzidine was 65%.

Reactions

TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.
Oxidation of TMB
Shows the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethylbenzidine diimine
The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at the wavelengths of 370 and 650 nm.

General Description

3,3',5,5'-tetramethylbenzidine appears as pale yellow crystals or off-white powder. (NTP, 1992)

Air & Water Reactions

Insoluble in water.

Reactivity Profile

Tetramethylbenzidine is sensitive to prolonged exposure to light . Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.

Fire Hazard

Flash point data for Tetramethylbenzidine are not available, however, Tetramethylbenzidine is probably combustible.

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in?situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Carcinogenicity

The carcinogenic potential of Tetramethylbenzidine is uncertain due to conflicting evidence. It is not mutagenic by the Ames test, and did not induce formation of tumors in a single-arm study of 24 rats. On that evidence, it has been used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.
DOI: 10.1177/26.2.24068

Tetramethylbenzidine synthesis

1004761-68-5
24596-19-8
54827-17-7
Synthesis of Tetramethylbenzidine from 2,6-Dimethyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline and 4-Bromo-2,6-dimethylaniline

Tetramethylbenzidine Preparation Products And Raw materials

Raw materials

ZINC Sodium dithionite potassium ferricyanide Ethanol Palladium
Polyphosphoric acid Copper(II) sulfate pentahydrate Diethyl ether Ammonium chloride Hydrogen
Dichloromethane Potassium permanganate Acetic acid Methanol 2,6-Dimethylaniline
Sulfuric acid

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Preparation Products

Tetramethylbenzidine Suppliers

Global( 543)Suppliers
Supplier Tel Email Country ProdList Advantage
Shanghai Daeyeon Chemicals Co., Ltd
021-64478606 +8615900664856 daeyeon001@vip.163.com China 2062 58
Suzhou Laing Biotechnology Co.,Ltd
+86-13004516015 +86-18149392822 christina@laingbiotec.com China 778 58
Suzhou BEC Biological Technology Co., Ltd.
+undefined13771885869 all@bek.com.cn China 62 58
Hebei Mojin Biotechnology Co., Ltd 		+8613288715578 sales@hbmojin.com China 12453 58
Zibo Hangyu Biotechnology Development Co., Ltd 		+86-0533-2185556 +8617865335152 Mandy@hangyubiotech.com China 11013 58
Henan Fengda Chemical Co., Ltd 		+86-371-86557731 +86-13613820652 info@fdachem.com China 7377 58
airuikechemical co., ltd. 		+undefined86-15315557071 sales02@airuikechemical.com China 994 58
Ouhuang Engineering Materials (Hubei) Co., Ltd 		+8617702722807 admin@hbouhuang.com China 343 58
Capot Chemical Co.,Ltd. 		571-85586718 +8613336195806 sales@capotchem.com China 29797 60
Shanghai Daken Advanced Materials Co.,Ltd 		+86-371-66670886 info@dakenam.com China 15371 58
Supplier Advantage
Shanghai Daeyeon Chemicals Co., Ltd
58
Suzhou Laing Biotechnology Co.,Ltd
58
Suzhou BEC Biological Technology Co., Ltd.
58
Hebei Mojin Biotechnology Co., Ltd 		58
Zibo Hangyu Biotechnology Development Co., Ltd 		58
Henan Fengda Chemical Co., Ltd 		58
airuikechemical co., ltd. 		58
Ouhuang Engineering Materials (Hubei) Co., Ltd 		58
Capot Chemical Co.,Ltd. 		60
Shanghai Daken Advanced Materials Co.,Ltd 		58

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View Lastest Price from Tetramethylbenzidine manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Tetramethylbenzidine pictures 2024-04-18 Tetramethylbenzidine
54827-17-7
 US $50.00 / kg 1kg 99.10% 5000kg Ouhuang Engineering Materials (Hubei) Co., Ltd
tetramethylbenzidine pictures 2024-04-10 tetramethylbenzidine
54827-17-7
 US $0.00-0.00 / Kg 1Kg 99.9% 200tons airuikechemical co., ltd.
Tetramethylbenzidine pictures 2024-03-31 Tetramethylbenzidine
54827-17-7
 US $30.00-20.00 / BOX 10BOX 99 20TONS Shanghai Chinqesen Biotechnology Co., Ltd.

Tetramethylbenzidine Spectrum

Tetramethylbenzidine(54827-17-7)MSTetramethylbenzidine(54827-17-7)1HNMRTetramethylbenzidine(54827-17-7)13CNMRTetramethylbenzidine(54827-17-7)IR1Tetramethylbenzidine(54827-17-7)IR2Tetramethylbenzidine(54827-17-7)Raman

54827-17-7(Tetramethylbenzidine)Related Search:

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3,3',5,5'-TETRAMETHYLBENZIDINE LIQUID SUBSTRATE SYSTEM 3,3',5,5'-TETRAMETHYLBENZIDINE SUBSTRATE 3,3',5,5'-TETRAMETHYLBENZIDIN 3,3',5',5-TETRAMETHYLBENZIDINE 3,3',5,5'-TETRAMETHYLBENZIDINE 3,3'-5,5-TETRAMETHYLBENZIDINE 3,3',5,5'-Tetramethylbenzidine [for Biochemical Research] 3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine TMB dihydrochloride3,3',5,5' Sure Blue TMB TMB Blotting Plus BM BLUE TETRAMETHYLBENZIDIN TETRAMETHYLBENZIDINE TMB+ ONE-STEP SUBSTRATE SYSTEM TMB QUICK TMB, SOLUBLE TMB, INSOLUBLE TMB LIQUID SUBSTRATE SYSTEM TMB/M 3,3,5,5,TETRAMETHYL BENZIDINE extrapure AR 3, 3μ,5 ,5μ-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA 4,4'-Bi-2,6-xylidine 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl TMB 3,3',5,5'-TETRAMETHYLBENZIDINE GR FOR AN 3,3',5,5'-TetraMethylbenzidine 99.5% 3,3,5,5-TetraMethyl benzidine solution liquid-1 coMponent 3,3',5,5'-TETRAMETHYLBENZIDINERESEARCH GRADE 1’-biphenyl)-4,4’-diamine,3,3’,5,5’-tetramethyl-( 1’-biphenyl)-4,4-diamine,3,3’,5,5’-tetramethyl-( 1’-biphenyl]-4,4’-diamine,3,3’,5,5’-tetramethyl-[ TMB-E TMB EASY TMB/H TMB-B TMB BLUE ONE-STEP SUBSTRATE SYSTEM 3,3',5,5'-TETRAMETHYLBENZIDINE TABLETS*F REE BASE 3,3',5,5'-TETRAMETHYLBENZIDINE, 99+% 3,3',5,5'-TETRAMETHYLBENZIDINE*FREE BASE 3,3',5,5'-TETRAMETHYLBENZIDINE SOLUTION Tmb,FreeBase TMB EASY, liquid substrate TMB QUICK, for ELISA, liquid substrate 3,3',5,5'-Tetramethyl 1,1-Biphenyl-4,4-diamine, 3,3,5,5-tetramethyl- 3,3,5,5-TETRAMETHYLBENZIDINE,REAGENT 3,3'',5,5''-TETRAMETHYLBENZIDINE(TMB) 3355TETRAMETHYL11BIPHENYL44DIAMINE 3,5,3'',5''-TETRAMETHYLBENZIDINE 99.5+% BIOLOGY GRADE 3,3'',5,5''-TETRAMETHYLBENZIDINE, TMB 99+% 3,3',5,5'-Tetramethylbenzidine, 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl 3,3',5,5'-TetraMethyl-4,4'-biphenyldiaMine 3.3'.5.5'-Tetr TMB color reagent B solution (peroxide solution) TMB Solution BM Blue POD Substrate, soluble 1’-Biphenyl]-4,4’-diamine,3,3’,5,5’-tetramethyl-[1 4,4'-BI-2,6-XYLIDINE 4,4'-DIAMINO-3,3',5,5'-TETRAMETHYLBIPHENYL