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Ethidium bromide

Ethidium bromide Structure
Ethidium bromide structure
CAS No.
1239-45-8
Chemical Name:
Ethidium bromide
Synonyms
ETBR;Ethidium bromide solution;HOMIDIUM BROMIDE;Rd1572;Dromilac;Homidium;-Cellstain-Eb;Ethidium Brmide;ETHIDIUM BROMIDE;EthidiumBromideGr
CBNumber:
CB7406991
Molecular Formula:
C21H20BrN3
Molecular Weight:
394.32
MDL Number:
MFCD00011724
MOL File:
1239-45-8.mol
MSDS File:
SDS
Last updated:2024-04-03 15:02:28
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Ethidium bromide Properties

Melting point 260-262 °C (dec.)(lit.)
Density 1.3739 (rough estimate)
refractive index 1.6700 (estimate)
Flash point >100°C
storage temp. 2-8°C
solubility H2O: 10 mg/mL, opaque, strongly red
form powder
color Red to dark purple
Odor Odorless solid
Water Solubility 40 g/L (25 ºC)
λmax 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm
Merck 14,4731
BRN 3642536
Stability Stable. Incompatible with strong oxidizing agents.
Biological Applications Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
Major Application Electroluminescent displays;photoresists
CAS DataBase Reference 1239-45-8(CAS DataBase Reference)
FDA UNII 059NUO2Z1L
EPA Substance Registry System Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1239-45-8)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictograms
GHS06,GHS08
Signal word  Danger
Hazard statements  H331-H341
Precautionary statements  P202-P261-P271-P280-P304+P340+P311-P308+P313
Hazard Codes  T,T+
Risk Statements  23-68-36/37/38-26-21/22-22
Safety Statements  36/37-45-36/37/39-28A-26-22-28-63
RIDADR  UN 2811 6.1/PG 1
WGK Germany  3
RTECS  SF7950000
F  8-9
HazardClass  6.1
PackingGroup  I
HS Code  29339990
Toxicity Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen.
NFPA 704
1
4 0

Ethidium bromide price More Price(41)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 46067 Ethidium bromide solution for fluorescence, ~1% in H2O 1239-45-8 50mL $124 2024-03-01 Buy
Sigma-Aldrich 46067 Ethidium bromide solution for fluorescence, ~1% in H2O 1239-45-8 250ML $492 2024-03-01 Buy
TCI Chemical E0370 Ethidium Bromide >95.0%(HPLC)(T) 1239-45-8 1g $107 2024-03-01 Buy
TCI Chemical E0370 Ethidium Bromide >95.0%(HPLC)(T) 1239-45-8 5g $504 2024-03-01 Buy
Alfa Aesar J62282 Ethidium bromide soln., 10mg/ml 1239-45-8 10ml $81 2024-03-01 Buy
Product number Packaging Price Buy
46067 50mL $124 Buy
46067 250ML $492 Buy
E0370 1g $107 Buy
E0370 5g $504 Buy
J62282 10ml $81 Buy

Ethidium bromide Chemical Properties,Uses,Production

description

Ethidium Bromide (EtBr), commonly used in research laboratories as a stain for the visualization of nucleic acids in electrophoresis gels, is a toxic chemical and a potent mutagen. When used in nucleic acid staining, ethidium bromide fluoresces a red-orange to pink color under ultraviolet light and with increased fluorescence when bound to double-stranded DNA. While it is not specifically regulated as a hazardous waste, the mutagenic properties may present health hazards and disposal concerns if it is not managed properly in the laboratory.

Uses

Ethidium bromide (EtBr) is a fluorescent dye widely used in molecular biology research. Early usage was as a veterinary trypanocide. It is a mutagenic compound that intercalates double-stranded DNA and RNA. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA, 25-fold on binding double-stranded DNA (although histones block binding of EtBr to DNA). Ethidium bromide has been used in multiple fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of the binding to DNA, EtBr is a powerful inhibitor of DNA polymerase.
Molecular Biology-grade Powder is suitable for use in gel electrophoresis and DNA isolation procedures.
Aqueous Solution (10 mg/mL) is suitable for use in gel electrophoresis and DNA isolation procedures.
Molecular Biology-grade Aqueous Solution (500 mg/mL) is suitable for use in gel electrophoresis.

Health Hazards

EtBr is a potent mutagen (may cause genetic damage), and moderately toxic after an acute exposure. EtBr can be absorbed through skin, so it is important to avoid any direct contact with the chemical. EtBr is an irritant to the skin, eyes, mouth, and upper respiratory tract. It should be stored away from strong oxidizing agents in a cool, dry place, and the container must be kept undamaged and tightly closed.

Chemical Properties

Purple/maroon crystalline powder

Uses

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).

Uses

antiprotozoal, intercalcates with DNA

Uses

Intercalating agent and fluorescent label for DNA.

Definition

ChEBI: Ethidium bromide is the organic bromide salt of ethidium. It has a role as a trypanocidal drug, a geroprotector and an intercalator. It contains an ethidium.

Health Hazard

Acute toxic effects from exposure to ethidium bromide have not been thoroughly investigated. Ethidium bromide is irritating to the eyes, skin, mucous membranes, and upper respiratory tract. Although there is no evidence for the carcinogenicity or teratogenicity of this substance in humans, ethidium bromide is strongly mutagenic and therefore should be regarded as a possible carcinogen and reproductive toxin

Flammability and Explosibility

Ethidium bromide does not pose a flammability hazard (NFPA rating = 1).

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Safety Profile

Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes.

storage

Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Operations capable of generating ethidium bromide dust or aerosols of ethidium bromide solutions should be conducted in a fume hood to prevent exposure by inhalation.

Purification Methods

Crystallise it from MeOH or EtOH [Lamos et al. J Am Chem Soc 108 4278 1986]. Its solubility in H2O is 1%. [Beilstein 22/11 V 352.] POSSIBLE CARCINOGEN.

Incompatibilities

No incompatibilities are known.

Waste Disposal

Excess ethidium bromide and waste material containing this substance should be placed in an appropriate container, clearly labeled, and handled according to your institution's waste disposal guidelines.

Ethidium bromide Preparation Products And Raw materials

Raw materials

Preparation Products

Ethidium bromide Suppliers

Global( 335)Suppliers
Supplier Tel Email Country ProdList Advantage
Henan Fengda Chemical Co., Ltd 		+86-371-86557731 +86-13613820652 info@fdachem.com China 7786 58
Henan Tianfu Chemical Co.,Ltd. 		+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21691 55
career henan chemical co 		+86-0371-86658258 sales@coreychem.com China 29914 58
Hubei Jusheng Technology Co.,Ltd. 		18871490254 linda@hubeijusheng.com CHINA 28180 58
Chongqing Chemdad Co., Ltd 		+86-023-61398051 +8613650506873 sales@chemdad.com China 39916 58
CONIER CHEM AND PHARMA LIMITED 		+8618523575427 sales@conier.com China 47465 58
Antai Fine Chemical Technology Co.,Limited 		18503026267 info@antaichem.com CHINA 9641 58
Zhengzhou Alfa Chemical Co.,Ltd 		+8618530059196 sale04@alfachem.cn China 12468 58
TargetMol Chemicals Inc. 		+1-781-999-5354 +1-00000000000 marketing@targetmol.com United States 19892 58
Hefei TNJ Chemical Industry Co.,Ltd. 		0551-65418671 sales@tnjchem.com China 34572 58
Supplier Advantage
Henan Fengda Chemical Co., Ltd 		58
Henan Tianfu Chemical Co.,Ltd. 		55
career henan chemical co 		58
Hubei Jusheng Technology Co.,Ltd. 		58
Chongqing Chemdad Co., Ltd 		58
CONIER CHEM AND PHARMA LIMITED 		58
Antai Fine Chemical Technology Co.,Limited 		58
Zhengzhou Alfa Chemical Co.,Ltd 		58
TargetMol Chemicals Inc. 		58
Hefei TNJ Chemical Industry Co.,Ltd. 		58

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View Lastest Price from Ethidium bromide manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Ethidium bromide pictures 2024-04-05 Ethidium bromide
1239-45-8
 US $8.00-1.00 / kg 1kg 99% g-kg-tons, free sample is available Henan Fengda Chemical Co., Ltd
Ethidium bromide pictures 2023-07-10 Ethidium bromide
1239-45-8
 US $583.00 / g 25g 95% 1000kg Jiangsu Boquan Biotechnology Co., Ltd.
Ethidium bromide pictures 2019-07-06 Ethidium bromide
1239-45-8
 US $1.00 / KG 1G 98% 100KG Career Henan Chemical Co
  • Ethidium bromide pictures
  • Ethidium bromide
    1239-45-8
  • US $583.00 / g
  • 95%
  • Jiangsu Boquan Biotechnology Co., Ltd.

Ethidium bromide Spectrum

Ethidium bromide(1239-45-8)MSEthidium bromide(1239-45-8)1HNMREthidium bromide(1239-45-8)IR1Ethidium bromide(1239-45-8)IR2

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