Nitrotetrazolium blue chloride

Nitrotetrazolium blue chloride Properties

Melting point	200°C
Density	1.5521 (rough estimate)
refractive index	1.7350 (estimate)
storage temp.	2-8°C
solubility	methanol: 50 mg/mL, clear, very strongly yellow
form	Powder/Solid
color	Yellow
Water Solubility	Soluble in water, ethanol and methanol.
Sensitive	Light Sensitive
λmax	256 nm
BRN	4115923
Stability	Stable, but store cold.
Biological Applications	Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
FDA UNII	X44P41F7ZK
EPA Substance Registry System	2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07,GHS08

Signal word

Danger

Hazard statements

H315-H319-H332-H360D

Precautionary statements

P201-P261-P264-P280-P304+P340+P312-P308+P313

Hazard Codes

Xn,Xi

Risk Statements

22-68/20/21/22-20/21/22-36-36/37/38-41

Safety Statements

36-45-36/37-26-39

WGK Germany

3

RTECS

XF8045000

F

8-10-23

TSCA

Yes

HS Code

32041900

Toxicity

LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990

NFPA 704

	1
2		0

Nitrotetrazolium blue chloride price More Price(46)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	N5514	Nitro Blue Tetrazolium tablet	298-83-9	10tablets	$88.5	2024-03-01	Buy
Sigma-Aldrich	N5514	Nitro Blue Tetrazolium tablet	298-83-9	25tablets	$173	2024-03-01	Buy
Sigma-Aldrich	484235	Nitrotetrazolium Blue chloride	298-83-9	250mg	$85.2	2024-03-01	Buy
Sigma-Aldrich	484235	p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC?? = 3-4 μM).	298-83-9	1g	$245	2024-03-01	Buy
Sigma-Aldrich	11585029001	NBT 4-Nitro blue tetrazolium chloride, crystals	298-83-9	5G	$742	2024-03-01	Buy

Product number	Packaging	Price	Buy
N5514	10tablets	$88.5	Buy
N5514	25tablets	$173	Buy
484235	250mg	$85.2	Buy
484235	1g	$245	Buy
11585029001	5G	$742	Buy

Nitrotetrazolium blue chloride Chemical Properties,Uses,Production

Description

Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

Chemical Properties

NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.

Uses

Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

Application

Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.

Definition

ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).

General Description

Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Hazard

Moderately toxic by ingestion.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Safety Profile

Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.

storage

+4°C

Nitrotetrazolium blue chloride Preparation Products And Raw materials

Raw materials

3,3'-Dimethoxybenzidine dihydrochloride Isoamyl nitrite Ethanol Benzaldehyde Tetrahydrofuran

4-Nitrophenylhydrazine

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Preparation Products

Nitrotetrazolium blue chloride Suppliers

Global( 435)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Suzhou Laing Biotechnology Co.,Ltd	+86-13004516015 +86-18149392822	christina@laingbiotec.com	China	778	58
Hebei Mojin Biotechnology Co., Ltd	+8613288715578	sales@hbmojin.com	China	12456	58
Shandong Juchuang Chemical Co., LTD	+86-18885615001 +86-18885615001	admin@juchuangchem.com	China	387	58
Shanghai Daken Advanced Materials Co.,Ltd	+86-371-66670886	info@dakenam.com	China	15928	58
Henan Tianfu Chemical Co.,Ltd.	+86-0371-55170693 +86-19937530512	info@tianfuchem.com	China	21691	55
Hefei TNJ Chemical Industry Co.,Ltd.	+86-0551-65418679 +86-18949832763	info@tnjchem.com	China	2989	55
career henan chemical co	+86-0371-86658258	sales@coreychem.com	China	29914	58
HaBo Hong Kong Co., Limited.	+86-25-18512596065	info@habotech.com	CHINA	933	58
Accela ChemBio Inc.	(+1)-858-699-3322	info@accelachem.com	United States	19965	58
Hebei Guanlang Biotechnology Co., Ltd.	+86-19930503282	alice@crovellbio.com	China	8823	58

Supplier	Advantage
Suzhou Laing Biotechnology Co.,Ltd	58
Hebei Mojin Biotechnology Co., Ltd	58
Shandong Juchuang Chemical Co., LTD	58
Shanghai Daken Advanced Materials Co.,Ltd	58
Henan Tianfu Chemical Co.,Ltd.	55
Hefei TNJ Chemical Industry Co.,Ltd.	55
career henan chemical co	58
HaBo Hong Kong Co., Limited.	58
Accela ChemBio Inc.	58
Hebei Guanlang Biotechnology Co., Ltd.	58

View Lastest Price from Nitrotetrazolium blue chloride manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2024-03-13	Nitrotetrazolium blue chloride 298-83-9	US $44.00-22.00 / kg	500kg	99.9	200tons	Shandong Juchuang Chemical Co., LTD
2023-09-24	Nitrotetrazolium blue chloride 298-83-9	US $0.00-0.00 / kg	1kg	98%	1000kg	Suzhou Laing Biotechnology Co.,Ltd
2023-09-06	Nitrotetrazolium blue chloride 298-83-9	US $0.00 / KG	1KG	99%	50000KG/month	Hebei Mojin Biotechnology Co., Ltd

Nitrotetrazolium blue chloride
298-83-9
US $44.00-22.00 / kg
99.9
Shandong Juchuang Chemical Co., LTD

Nitrotetrazolium blue chloride
298-83-9
US $0.00-0.00 / kg
98%
Suzhou Laing Biotechnology Co.,Ltd

Nitrotetrazolium blue chloride
298-83-9
US $0.00 / KG
99%
Hebei Mojin Biotechnology Co., Ltd

Nitrotetrazolium blue chloride Spectrum

Nitrotetrazolium blue chloride(298-83-9)¹HNMR Nitrotetrazolium blue chloride(298-83-9)IR1 Nitrotetrazolium blue chloride(298-83-9)IR2

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M-NITRO CHLORO BENZENE(MNCB) p-Nitro blue tetrazolium, diformazan p-NBT, diformazan TETRAZOLE BLUE DIFORMAZAN Nitro Blue Tetrazolium Chloride

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2h-tetrazolium,3,3’-(3,3’-dimethoxy[1,1’-biphenyl]-4,4’-diyl)bis[2-(4-nitrophe nbt(dye) nitrobluetetrazoliumsalt nitrotetrazoliumbt nitrotetrazoliumchlorideblue tetrazoliumnitroblue tetrazoliumnitrobt 2H-TETRAZOLIUM,2,2'-((3,3')-DIMETHOXY[1,1'-BIPHENYL]-4,4'-DIYL)BIS[3-(4-NITROPHENYL)-5-PHENYL-],DICHLORIDE 3,3'-(3,3'-DIMETHOXY[1,1'-BIPHENYL]-4,4'-DIYL)BIS[2-(4-NITROPHENYL)]-5-PHENYL-2H-TETRAZOLIUM DICHLORIDE 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE] 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)-BIS-(2-P-NITROPHENYL)-5-(PHENYL)-2H-TETRAZOLIUM CHLORIDE 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM] CHLORIDE 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLYLENE)BIS[2-(P-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE] 2,2'-DI-4-NITROPHENYL-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)-DITETRAZOLIUM-CHLORIDE 2,2'-BIS(4-NITROPHENYL)-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)DITETRAZOLIUM CHLORIDE 2,2'-DI-P-NITROPHENYL-5,5'-DIPHENYL-(3,3'-DIMETHOXY)-4,4'-BISPHENYLENEDITETRAZOLIUM CHLORIDE 2,2'-DI-P-NITROPHENYL-5,5'DIPHENYL-[3,3'-DIMETHOXY-4,4'-DIPHENYLENE] DITETRAZOLIUM CHLORIDE DITETRAZOLIUM DYE P-NITROBLUETETRAZOLIUMCHLORIDE,CRYSTAL,REAGENT NITROBLUETETRAZOLIN Nitro BT (Nitro blue tetrazolium chloride) NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE extrapure AR p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride NBT Vials p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, Nitrotetrazolium Blue chloride, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2) 3,3'-[3,3-Dimethoxy-(1,1-biphenyl)-4,4-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride] NBTMF Nitro blue tetrazolium >99% Nitro Blue Tetrazolium chloride, tech. 90% Nitro Blue Tetrazolium [for Biochemical Research] Nitro Blue Tetrazolium Chloride monohydrate,90% Nitro Blue Tetrazolium Chloride monohydrate,85% NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE: TECH., 90% 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'- biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl -, dichloride 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy- p-Nitro-Blue tetrazolium chlo Nitrotetrazolium Blue chloride ,90% 2-(3,3'-Dimethoxy-4'-(2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium-3-yl)-[1,1'-biphenyl]-4-yl) p-Nitro-Blue tetrazolium chlori 2h-tetrazolium,3,3’-(3,3’-dimethoxy(1,1’-biphenyl)-4,4’-diyl)bis(2-(4-nitrophe 2h-tetrazolium, 2,20-(3,30-dimethoxy [1,10-biphenyl]-4,40-diyl)bis[3-(4-nitrophenyl)-5-phenyl]-, dichloride 2h-tetrazolium, 3,30-(3,30-dimethoxy- 4,40-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl]-, dichloride Tetrazolium, 3,30-(3,30-dimethoxy-4,40-biphenylylene) bis[2-(p-nitrophenyl)-5-phenyl-2h-chloride] 2, 20-bis(p-nitrophenyl)-5,50-diphenyl-3,30-(3,30-dimethoxy- 4,40-biphenylylene)ditetrazolium Chloride 2,20-bis(p-nitrophenyl)- 5,50-diphenyl-3,30-(3,30-dimethoxy-4,40-diphenylene) ditetrazolium Chloride 2,20-di-p-nitrophenyl- 5,50-diphenyl-3,30-bis(3,30-dimethoxy-4,40-biphenylene) ditetrazolium Chloride 2,20-dinitrophenyl-5,50-diphenyl- 3,30-dimethoxy-4,40-diphenylene)ditetrazolium Chloride 3,30-(3,30-dimethoxy-4,40-biphenylylene)bis[2-(p-nitrophenyl)- 5-phenyl-2h-tetrazolium Chloride] 3,30-(3,30- dimethoxy-4,40-diphenylene)bis[2-(p-nitrophenyl)-5- phenyltetrazolium Chloride] Nsc 27622 P- nitrotetrazolium Blue Nitro Blue Tetrazolium (nbt) NBT CHLORIDE NITRO BLUE TETRAZOLIUM (NBT) REDUCTION NITRO TETRAZOLIUM BLUE NITROTETRAZOLIUM BLUE CHLORIDE P-NITRO-BLUE TETRAZOLIUM CHLORIDE P-NITROBLUE TETRAZOLIUM DICHLORIDE