プロテイナーゼK

プロテイナーゼK 価格 もっと(50)

メーカー	製品番号	製品説明	CAS番号	包装	価格	更新時間	購入
富士フイルム和光純薬株式会社(wako)	W01MPB02193504	プロティナーゼK, トリチラキウム属製 Proteinase K, From Tritirachium album	39450-01-6	25mg	￥6900	2024-03-01	購入
富士フイルム和光純薬株式会社(wako)	W01MPB02193504	プロティナーゼK, トリチラキウム属製 Proteinase K, From Tritirachium album	39450-01-6	100mg	￥14500	2024-03-01	購入
Sigma-Aldrich Japan	SRE0005	プロテイナーゼ K from Tritirachium album Reagents designed and manufactured under current ISO 13485 certification. Reagents designed and manufactured under current ISO 13485 certification.	39450-01-6	250ML	￥1910000	2024-03-01	購入
Sigma-Aldrich Japan	P2308	プロテイナーゼ K from Tritirachium album lyophilized powder, BioUltra, ≥30?units/mg protein, for molecular biology Proteinase?K from Tritirachium album lyophilized powder, BioUltra, ≥30?units/mg protein, for molecular biology	39450-01-6	5mg	￥15600	2024-03-01	購入
Sigma-Aldrich Japan	P2308	プロテイナーゼ K from Tritirachium album lyophilized powder, BioUltra, ≥30?units/mg protein, for molecular biology Proteinase?K from Tritirachium album lyophilized powder, BioUltra, ≥30?units/mg protein, for molecular biology	39450-01-6	10mg	￥17900	2024-03-01	購入

プロテイナーゼK MSDS

Prok

プロテイナーゼK 化学特性,用途語,生産方法

外観

無色〜わずかにうすい黄色, 澄明の液体

溶解性

水に溶ける。

用途

DNA及びRNAの精製。

特長

疎水性脂質族および芳香族アミノ酸のカルボキシル基を含むペプチド結合を主に分解する。多くのDNA、RNA分解酵素を急速に分解し不活化する作用があるので、核酸の精製に使用される。本品は、遺伝子工学用の製品であるため核酸の抽出に最適である。

説明

Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in the absence of this divalent cation some catalytic activity is retained. Maximum proteinase K activity is observed with the inclusion of 1 mM Ca++ in the reaction buffer. Proteinase K digestion is routinely performed at 50°C, and occasionally in the presence of EDTA to inhibit labile, Mg++-dependent nucleases. Proteinase K is prepared commonly as a 20 mg/mL stock solution in sterile water (stable for 1 year at ?20°C) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl2 (stable for months at 4°C). It is generally used at a working concentration of up to 50 μg/mL in any of a number of buffer formulations, including those that contain as much as 0.5% SDS[1].

化学的特性

Powder beige

使用

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

定義

Proteinase K cleaves peptide bonds next to the carboxyl-terminal of aromatic amino acids, hydrophobic amino acids and sulfuric amino acids, within the polypeptide chain. Hence Proteinase K can act as an endoprotease on a wide range of protein substrates. Because of its proteolytic preferences, it is a member of the subtilisin group of serine proteases. The K in Proteinase K refers to its ability to digest Keratin protein found in hairs, nails and hooves. Proteinase K is an essential reagent in molecular biology labs because it is useful in isolating DNA and RNA molecules by degrading and inactivating proteins. Fungus Tritirachium album Limber naturally produces proteinase K.

一般的な説明

Proteinase K, an extracellular endopeptidase is synthesized by the mold, Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein and is characterized with an unhydrolyzed protein chain and autolyzed polypeptide chains.

プロテイナーゼK 上流と下流の製品情報

原材料

準備製品

プロテイナーゼK 生産企業

名前	電話番号	電子メール	国籍	製品カタログ	優位度
Henan Alfa Chemical Co., Ltd	+8618339805032	alfa4@alfachem.cn	China	12756	58
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	47465	58
Jiangsu Boquan Biotechnology Co., Ltd.	+86-18168774353	boquanshengwu003@boquansw.com	China	248	58
Xi’an Sengmei Biotech Co.,Ltd.	+8615398038360	lionel@accenturebio.com	China	288	58
XI AN SGONEK BIOLOGICAL TECHNOLOGY CO., LTD	15202951039	jason@sgonekbio.com	China	268	58
LEAPCHEM CO., LTD.	+86-852-30606658	market18@leapchem.com	China	43348	58
Hebei Mojin Biotechnology Co., Ltd	+8613288715578	sales@hbmojin.com	China	12452	58
Hebei Yanxi Chemical Co., Ltd.	+8617531190177	peter@yan-xi.com	China	5993	58
Anhui Ruihan Technology Co., Ltd	+8617756083858	daisy@anhuiruihan.com	China	994	58
Hebei Jingbo New Material Technology Co., Ltd	+8619931165850	hbjbtech@163.com	China	1000	58

39450-01-6(プロテイナーゼK)キーワード:

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• 39450-01-6
• ProteinaseK,PowderAndSolutionAvailable
• ProteinaseK,fromTritirachiumalbumLimber
• Proteinase K from Tritirachium album ca. 8 DMC-U/mg
• PROTEINASEK,LYOPHILIZEDPOWDER,BIOTECHGRADE
• Proteinase K for tritirachium album serine.
• Proteinase K from Tritirachium album [EC 3.4.21.64] from Tritirachium album
• proteinase k for tritirachium album
• Proteinase K, tritirachium album serine
• Proteinase Ksolution,from Tritirachium album
• Einecs 254-457-8
• PROTEINASE K FR. TRITIRACHIUMALBUM CA.8 DCM-U/MG LYOPH.
• Proteinase K from Tritirachium album,Endopeptidase K
• Proteinase K Solution, 20 mg/ml
• 40U/MG
• Proteinase K froM TritirachiuM albuM ca. 8 DMC-U/Mg lyophil.
• RecoMbinant Proteinase K
• Proteinase K from Tritirachium album lyophil.
• KProteinas
• KProteinase K
• Proteinase K from Tritirachium album Vetec(TM) reagent grade, powder, >=30 units/mg protein
• BOTTLE 1L SMALL BROWN GLASS DIN45
• Endopeptidase K Prok Protease K Tritirachium album proteinase K
• IUB: 3.4.21.14
• IUB: 3.4.21.64
• EC 3.4.21.64
• ENDOPEPTIDASE K
• PROTEINASE K
• PROTEINASE K, LYOPHILIZED
• PROTEINASE K TRITIRACHIUM ALBUM
• PROTEINASE K, IMMOBILIZED
• プロテイナーゼK
• プロティナーゼK溶液
• プロティナーゼK, トリチラキウム属製
• プロティナーゼK
• プロテイナーゼ K FROM TRITIRACHIUM ALBUM