エノラーゼ、酵母製 化学特性,用途語,生産方法
外観
タンパク質1～30mg/ml, りん酸カリウム, 0.05～0.1mol/l, 50%グリセリン溶液(pH7.0±0.5)
使用
The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.
定義
An enzyme active in glycolysis that catalyzes the conversion of 2-phosphoglyceric acid to the phosphorylated enol of pyruvic acid.
一般的な説明
Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg
2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes.
エノラーゼ、酵母製 上流と下流の製品情報
原材料
準備製品