XBAI

XBAI 価格 もっと(11)

メーカー	製品番号	製品説明	CAS番号	包装	価格	更新時間	購入
関東化学株式会社(KANTO)	49890-74	XbaI		3000units	￥11200	2024-03-01	購入
関東化学株式会社(KANTO)	49890-73	XbaI		15000units	￥46000	2024-03-01	購入
Sigma-Aldrich Japan	XBAI-RO	from Xanthomonas campestris Xba I from Xanthomonas campestris		1000UNITS	￥6350	2024-03-01	購入
Sigma-Aldrich Japan	XBAI-RO	from Xanthomonas campestris Xba I from Xanthomonas campestris		5000UNITS	￥25000	2024-03-01	購入
Sigma-Aldrich Japan	XBAI-RO	from Xanthomonas campestris Xba I from Xanthomonas campestris		20000UNITS	￥77600	2023-06-01	購入

XBAI 化学特性,用途語,生産方法

使用

The restriction enzyme Xba I has been used for the digestion of genomic DNA.

一般的な説明

Xba I recognizes the sequence T↓*CTAGA and generates fragments with 5′-cohesive termini. The enzyme needs at least two nucleotides around the target sequence before it will cut the cleavage site.

Compatible ends
Xba I ends are compatible with fragments generated by Avr I, Nhe I, and Spe I.

Isoschizomers
The enzyme is not known to have isoschizomers.

Methylation sensitivity
Xba I digestion of DNA is inhibited by the dam gene product of E. coli, which methylates the 6N position of adenine in the sequence GATC. The enzyme is also inhibited by 5-methylcytosine or 5-hydroxymethylcytosine at the site (*) indicated on the recognition sequence.

Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:
A B L M H
100% 75-100% 75-100% 75-100% 100%

Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 60%. The PCR mix contained ?DNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl2, 200 ?M dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles. Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 25%. When supplemented with GC-RICH Solution, activity increases to 100%.

Incubation temperature
+37°C


Number of cleavage sites on different DNAs
λ Ad2 SV40 ?X174 M13mp7 M13mp18 pBR322 pBR328 pUC18
1 5 0 0 0 1 0 0 1

PFGE tested
Xba I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E. coli C 600) embedded in agarose for PFGE analysis, we recommend 10 U enzyme per ?g DNA and approximately 4 hour incubation time.

Ligation and recutting assay
Xba I fragments obtained by complete digestion of 1 ?g pUC18 DNA are ligated with 1 U T4 DNA Ligase in a volume of 10 ?l by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >90% recovery of pUC18 DNA.
Subsequent re-cutting with Xba I yields >95% of the typical pattern of pUC18 × Xba I fragments.

XBAI 上流と下流の製品情報

原材料

準備製品

XBAI 生産企業

名前	電話番号	電子メール	国籍	製品カタログ	優位度
FERMENTAS Inc.	--		United States	335	82
Amersham Biosciences, Inc.	--		United States	1270	85
Nacalai Tesque, Inc.	--	info-tech@nacalai.co.jp	Japan	6048	75
Roche Applied Science	--	biochemts.us@roche.com	United States	696	85
Qbiogene	--	orders@qbiogene.com	United States	925	69
MP Biomedicals, Inc.	--		United States	6566	81

(XBAI)キーワード:

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• XBA I
• XBAI