フルオレセイン

フルオレセイン 化学構造式
2321-07-5
CAS番号.
2321-07-5
化学名:
フルオレセイン
别名:
3,6-ジヒドロキシスピロ[9H-キサンテン-9,1'(3'H)-イソベンゾフラン]-3'-オン;D&CイエローNo.7;C.I.ソルベントイエロー94;2-(3-ヒドロキシ-6-オキソ-6H-キサンテン-9-イル)安息香酸;2-[(6-ヒドロキシ-3-オキソ-3H-キサンテン)-9-イル]安息香酸;DandCイエロー7;2-(3-オキソ-6-ヒドロキシ-3H-キサンテン-9-イル)安息香酸;2-(6-ヒドロキシ-3-オキソ-3H-キサンテン-9-イル)安息香酸;レゾルシノールフタレイン;レソルシノールフタレイン;3',6'-ジヒドロキシスピロ[イソベンゾフラン-1(3H),9'-[9H]キサンテン]-3-オン;9-(o-カルボキシフェニル)-6-ヒドロキシ-3H-キサンテン-3-オン;2-(3,6,9-トリヒドロキシ-9H-キサンテン-9-イル)安息香酸γ-ラクトン;黄色201号;黄201;アシッドイエロー73;アシッドエロー73;フルオレセイン REAG. PH. EUR.,FREE ACID;フルオレセイン, HIGH PURITY STANDARD;フルオレセイン 蛍光標準品
英語名:
Fluorescein
英語别名:
SOLVENT YELLOW 94;CI 45350:1;Fluorecein;fluoresceine;KI201;S NO 880;YELLOW 7;CI 45350.1;NSC 667256;angiofluor
CBNumber:
CB7753558
化学式:
C20H12O5
分子量:
332.31
MOL File:
2321-07-5.mol
MSDS File:
SDS

フルオレセイン 物理性質

融点 :
320 °C(lit.)
沸点 :
429.44°C (rough estimate)
比重(密度) :
1.2739 (rough estimate)
屈折率 :
1.5000 (estimate)
貯蔵温度 :
room temp
溶解性:
水、エーテル、ベンゼン、クロロホルムに溶けず、エタノール、メタノール、アセトン、酢酸エチル、N,N-ジメチルホルムアミドに溶ける。
カラーインデックス :
45350
外見 :
酸解離定数(Pka):
2.2, 4.4, 6.7(at 25℃)
色:
赤からオレンジ
酸塩基指示薬変色域(pH):
Pink uorescence (4.0) to green uorescence (6.0)
水溶解度 :
不溶性
極大吸収波長 (λmax):
493.5nm, 496nm, 460nm, 515nm
Merck :
14,4159
BRN :
94324
安定性::
安定。可燃性。強力な酸化剤とは相容れない。
主な用途:
Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
生物学的応用:
Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
LogP:
2.980 (est)
CAS データベース:
2321-07-5(CAS DataBase Reference)
EPAの化学物質情報:
Fluorescein (2321-07-5)
安全性情報
  • リスクと安全性に関する声明
  • 危険有害性情報のコード(GHS)
主な危険性  Xi
Rフレーズ  43-36-36/37/38
Sフレーズ  22-24/25-37/39-26-36/37/39-27
WGK Germany  3
RTECS 番号 LM5075000
10-21
TSCA  Yes
HSコード  32042000
有毒物質データの 2321-07-5(Hazardous Substances Data)
化審法 (5)-671, (5)-1416, (5)-3060
絵表示(GHS) GHS hazard pictograms
注意喚起語 警告
危険有害性情報
コード 危険有害性情報 危険有害性クラス 区分 注意喚起語 シンボル P コード
H319 強い眼刺激 眼に対する重篤な損傷性/眼刺激 性 2A 警告 GHS hazard pictograms P264, P280, P305+P351+P338,P337+P313P
注意書き
P305+P351+P338 眼に入った場合:水で数分間注意深く洗うこと。次にコ ンタクトレンズを着用していて容易に外せる場合は外す こと。その後も洗浄を続けること。

フルオレセイン 価格 もっと(49)

メーカー 製品番号 製品説明 CAS番号 包装 価格 更新時間 購入
富士フイルム和光純薬株式会社(wako) W01AFAL13251 フルオレセイン, 90+%
Fluorescein, 90+%
2321-07-5 100g ¥12260 2024-03-01 購入
富士フイルム和光純薬株式会社(wako) W01AFAL13251 フルオレセイン, 90+%
Fluorescein, 90+%
2321-07-5 500g ¥38840 2024-03-01 購入
東京化成工業 F0095 フルオレセイン
Fluorescein
2321-07-5 25g ¥3200 2024-03-01 購入
東京化成工業 F0095 フルオレセイン
Fluorescein
2321-07-5 100g ¥6400 2024-03-01 購入
関東化学株式会社(KANTO) 11924-2A フルオレセイン
Fluorescein
2321-07-5 25g ¥4700 2024-03-01 購入

フルオレセイン 化学特性,用途語,生産方法

外観

黄赤色~赤褐色、粉末

定義

本品は、平成15年厚労省令第126号に示される黄色201号である。

溶解性

希アンモニア水に可溶

解説

フルオレセイン,黄赤色の粉末.融点314~316 ℃.水に不溶,エタノールに微溶.アルカリ性水溶液に溶けて黄赤色を呈し,強い黄緑色の蛍光を発する.二ナトリウム塩はウラニン(uranin)またはウラニンイエロー(I.C.Acid Yellow 73)とよばれる.吸収極大510 nm,蛍光極大510 nm.染料,顔料,およびその中間体.印刷インキに用いられるほか,蛍光を利用し,地中水路の探索,生化学反応の追跡,医療(内科,外科,眼科)上の検査などにプローブとして広く利用される.ナトリウム塩はLD50 6721 mg/kg(ラット,経口).

森北出版「化学辞典(第2版)

用途

蛍光造影作用を有しています。

用途

蛍光造影作用を有しています

用途

蛍光染料。pHプローブ、光学的なpHセンサー。

製造

レソルシノール 2モル量と無水フタル酸1モル量とを塩化亜鉛の存在下,210 ℃ に加熱縮合して合成するフルオレセイン.

化粧品の成分用途

着色剤

効能

診断補助薬 (角膜外傷指標)

説明

Fluorescein is a synthetic organic compound available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications.
Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 521 nm (in water). The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite.
Fluorescein also has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein is also known as a color additive (D&C Yellow no. 7). The disodium salt form of fluorescein is known as uranine or D&C Yellow no. 8.
The color of its aqueous solution varies from green to orange as a function of the way it is observed: by reflection or by transmission, as it can be noticed in bubble levels in which fluorescein is added as a colorant to the alcohol filling the tube to increase the visibility of the air bubble and the precision of the instrument. More concentrated solutions of fluorescein can even appear red.

化学的特性

Orange-red, crystalline powder. Very dilute alkaline solutions exhibit intense greenishyellow fluorescence by reflected light, while the solution is reddish-orange by transmitted light.soluble in dilute alkalies, boiling alcohol, ether, dilute acids, and

物理的性質

The fluorescence of this molecule is very intense; peak excitation occurs at 494 nm and peak emission at 521 nm.
Fluorescein has a pKa of 6.4, and its ionization equilibrium leads to pH-dependent absorption and emission over the range of 5 to 9. Also, the fluorescence lifetimes of the protonated and deprotonated forms of fluorescein are approximately 3 and 4 ns, which allows for pH determination from non intensity based measurements. The lifetimes can be recovered using time-correlated single photon counting or phase-modulation fluorimetry.

使用

Fluorescein is used as a fluorescent tracer for many applications including in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains and in dye tracing. It is used to localise multiple muscular ventricular septal defects during open heart surgery and confirm the presence of any residual defects. It is applied to teeth to reveal plaque.

主な応用

Biochemical research
In cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence microscopy applications (for example, flow cytometry). Additional biologically active molecules (such as antibodies) may also be attached to fluorescein, allowing biologists to target the fluorophore to specific proteins or structures within cells. This application is common in yeast display.
Health care applications
"Fluorescein sodium", the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and optometry, where topical fluorescein is used in the diagnosis of corneal abrasions, corneal ulcers and herpetic corneal infections. It is also used in rigid gas permeable contact lens fitting to evaluate the tear layer under the lens.
Uses in river systems
In 1966, environmentalists forced a change to a vegetable-based dye to protect local wildlife. Other uses of fluorescein include using it as a water-soluble dye added to rainwater in environmental testing simulations to aid in locating and analyzing any water leaks, and in Australia and New Zealand as a methylated spirit dye.
Oil field application
Fluorescein dye solutions, typically 15 % active, are commonly used as an aid to leak detection during hydrostatic testing of sub sea oil and gas pipelines and other subsea infrastructure. Leaks can be detected by divers carrying ultraviolet lights.

定義

ChEBI: A xanthene dye that is highly fluorescent, detectable even when present in minute quantities. Used forensically to detect traces of blood, in analytical chemistry as an indicator in silver nitrate titrations and in microscopy.

一般的な説明

Yellow amorphous solid or orange-red crystals. Latter have greenish-yellow fluorescence by reflected light. Insoluble in water. Soluble in dilute aqueous bases. Very dilute alkaline solutions exhibit intense, greenish-yellow fluorescence by reflected light. Low toxicity. May be sensitive to prolonged exposure to light.

空気と水の反応

Insoluble in water.

反応プロフィール

Fluorescein is incompatible with strong oxidizers. Also incompatible with acids, acid salts and salts of heavy metals. .

火災危険

Flash point data for Fluorescein is not available, but Fluorescein is probably combustible.

安全性プロファイル

Poison by intravenous route. Moderately toxic by intraperitoneal route. Mutation data reported. When heated to decomposition it emits acrid smoke and irritating fumes. See also FLUORESCEIN SODIUM.

安全性

Topical, oral, and intravenous use of fluorescein can cause adverse reactions, including nausea, vomiting, hives, acute hypotension, anaphylaxis and related anaphylactoid reaction, causing cardiac arrest and sudden death due to anaphylactic shock.
The most common adverse reaction is nausea, due to a difference in the pH from the body and the pH of the sodium fluorescein dye; a number of other factors , however, are considered contributors as well. The nausea usually is transient and subsides quickly. Hives can range from a minor annoyance to severe, and a single dose of antihistamine may give complete relief. Anaphylactic shock and subsequent cardiac arrest and sudden death are very rare, but because they occur within minutes, a health care provider who uses fluorescein should be prepared to perform emergency resuscitation.

フルオレセイン 上流と下流の製品情報

原材料

準備製品


フルオレセイン 生産企業

Global( 402)Suppliers
名前 電話番号 電子メール 国籍 製品カタログ 優位度
Shenyang Simchoice Chemical Co.,Ltd
+86-024-23769576 +86-15040101888
sales@simchoicechem.com China 254 58
Henan Fengda Chemical Co., Ltd
+8613613820652
info@fdachem.com China 4685 58
Shanghai Daken Advanced Materials Co.,Ltd
+86-371-66670886
info@dakenam.com China 14770 58
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512
info@tianfuchem.com China 21703 55
Hangzhou FandaChem Co.,Ltd.
008657128800458; +8615858145714
fandachem@gmail.com China 9359 55
ATK CHEMICAL COMPANY LIMITED
+undefined-21-51877795
ivan@atkchemical.com China 32480 60
Hefei TNJ Chemical Industry Co.,Ltd.
+86-0551-65418679 +86-18949832763
info@tnjchem.com China 2989 55
career henan chemical co
+86-0371-86658258
sales@coreychem.com China 29919 58
Hubei Jusheng Technology Co.,Ltd.
18871490254
linda@hubeijusheng.com CHINA 28180 58
Chongqing Chemdad Co., Ltd
+86-023-61398051 +8613650506873
sales@chemdad.com China 39916 58


2321-07-5(フルオレセイン)キーワード:


  • 2321-07-5
  • Fluorescein, C.I. 45350, synthesis grade
  • Fluorescein, C.I. 45350, extra pure
  • FluoresceinC.I.45350
  • Fluorescein, laser grade, pure, 99%
  • Fluorescein, pure
  • Fluorescein,highpuritystandard
  • D&CYellow#7
  • Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-
  • Fluorescein, 90+%
  • Deoxyribonucleic acid, DNA-length standard II, DNA-molecular weight marker II
  • Molecular Weight Marker II for DNA
  • Deoxyribonucleic acid, DNA-length standard I, DNA-molecular weight marker I
  • Deoxyribonucleic acid, DNA-length standard III, DNA-molecular weight marker III
  • Molecular Weight Marker III for DNA
  • 1R,4S)-1,3,3-Trimethylbicyclo[2.2.1]heptan-2-one
  • Diresorcinolphthalein
  • Tetraoxyphthalophenone anhydride
  • S NO 880
  • RESORCINOL PHTHALEIN
  • FLUORESCEIN, ALCOHOL SOLUBLE
  • FLUORESCEIN
  • CI 45350.1
  • CI NO 45350
  • D AND C YELLOW NO 7
  • 3,6-dihydroxyspiro[isobenzofuran-1(3H),9-xanthen]-3-one
  • Fluorescein Free Acid
  • KI201
  • Fluorescein (200 mg)
  • NSC 667256
  • FLUORESCEIN (C.I.45350) 25 G
  • 3,6-ジヒドロキシスピロ[9H-キサンテン-9,1'(3'H)-イソベンゾフラン]-3'-オン
  • D&CイエローNo.7
  • C.I.ソルベントイエロー94
  • 2-(3-ヒドロキシ-6-オキソ-6H-キサンテン-9-イル)安息香酸
  • 2-[(6-ヒドロキシ-3-オキソ-3H-キサンテン)-9-イル]安息香酸
  • DandCイエロー7
  • 2-(3-オキソ-6-ヒドロキシ-3H-キサンテン-9-イル)安息香酸
  • 2-(6-ヒドロキシ-3-オキソ-3H-キサンテン-9-イル)安息香酸
  • レゾルシノールフタレイン
  • レソルシノールフタレイン
  • 3',6'-ジヒドロキシスピロ[イソベンゾフラン-1(3H),9'-[9H]キサンテン]-3-オン
  • 9-(o-カルボキシフェニル)-6-ヒドロキシ-3H-キサンテン-3-オン
  • 2-(3,6,9-トリヒドロキシ-9H-キサンテン-9-イル)安息香酸γ-ラクトン
  • 黄色201号
  • 黄201
  • アシッドイエロー73
  • アシッドエロー73
  • フルオレセイン REAG. PH. EUR.,FREE ACID
  • フルオレセイン, HIGH PURITY STANDARD
  • フルオレセイン 蛍光標準品
  • フルオレセイン, 90+%
  • フルオレセイン標準品
  • フルオロセイン(遊離酸)
  • フルオレセイン (JAN)
  • フルオレセイン
  • 3′,6′-ジヒドロキシスピロ[イソベンゾフラン-1(3H),9′-[9H]キサンテン]-3-オン
  • 3′,6′-フルオランジオール
  • 6-ヒドロキシ-9-(2-カルボキシフェニル)-3H-キサンテン-3-オン
  • 9-(2-カルボキシフェニル)-6-ヒドロキシ-3H-キサンテン-3-オン
  • 9-(2-カルボキシフェニル)-3-オキソ-6-ヒドロキシ-3H-キサンテン
  • o-(3-オキソ-6-ヒドロキシ-3H-キサンテン-9-イル)安息香酸
  • 3,6-ジヒドロキシスピロ[9H-キサンテン-9,1′(3′H)-イソベンゾフラン]-3′-オン
  • フルオレセイン酸
  • 3′,6′-ジヒドロキシフルオラン
  • イエローフロオレセイン
  • 3',6'-ジヒドロキシ-3H-スピロ[2-ベンゾフラン-1,9'-キサンテン]-3-オン
  • 診断用薬
Copyright 2017 © ChemicalBook. All rights reserved