プロテインジスルフィドイソメラーゼ ウシ肝臓由来 化学特性,用途語,生産方法
使用
Protein Disulfide Isomerase from bovine liver has been used:
- to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and?L. chagasi
- in the in vitro translation reaction for the generation of disulfide bonds
- in insulin-disulfide reduction assay and peptide binding assay
- as a positive control in thiol-disulfide oxidoreductase activity assay
一般的な説明
Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2. The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose,
N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucose. PDI is an ubiquitous redox chaperone enzyme. It belongs to the, thioredoxin superfamily and is high conserved.
Biochem/physiol Actions
Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges. It aids protein refolding in vitro allowing recombinant proteins to achieve their native state.
プロテインジスルフィドイソメラーゼ ウシ肝臓由来 上流と下流の製品情報
原材料
準備製品