N-(3α,7α,12α-トリヒドロキシ-24-オキソ-5β-コラン-24-イル)グリシン 化学特性,用途語,生産方法
解説
グリセロリン酸3種類の異性体が考えられるが，リン脂質や中性脂肪の骨格をなすのはL-グリセリン3-リン酸で，加水分解中にリン酸基の転位により，一部2-リン酸になる．シロップ状の物質．水，メタノール，エタノールに易溶．グリセリンの水酸基1個とリン酸とがエステル様に結合してできたもので，リン酸基の位置によってα体とβ体とがある。グリセリン発酵の際，グリセロリン酸脱水素酵素によって，ジオキシアセトンリン酸から可逆的に生成される。
説明
Glycocholic acid is a glycine-conjugated form of the primary bile acid cholic acid and has roles in the emulsification of fats. It reduces expression of the gene encoding the farnesoid X receptor (FXR) and increases expression of the genes encoding the bile acid receptors TGR5 and S1PR2 in SNU-245 cells when used at a concentration of 1.6 μmol/ml. Glycocholic acid (250 μM) increases the intracellular accumulation and cytotoxicity of epirubicin in Caco-2 cells, as well as decreases expression of the genes encoding multidrug resistance protein 1 (MDR1), MDR-associated protein 1 (MRP1), and MRP2 when used alone or in combination with epirubicin. It increases absorption of epirubicin into everted sacs of rat ileum and jejunum when used at a concentration of 250 μM. The bile acid composition ratio of glycocholic acid is elevated in bile of patients with cholangiocarcinoma compared with patients with pancreatic cancer or benign biliary diseases. Serum levels of glycocholic acid are elevated in patients with hepatocellular carcinoma compared with healthy individuals.
化学的特性
Off-White Solid
使用
Labelled Glycocholic Acid. The product of conjugation of cholic acid with glycine; chief ingredient of the bile of herbivorous animals. In the weakly alkaline bile fluid glycocholic acid exists as the sodium salt.
定義
ChEBI: A bile acid glycine conjugate having cholic acid as the bile acid component.
純化方法
Glycocholic acid crystallises from hot water as the sesquihydrate. Dry it at 110o in vacuo. An analytical sample is prepared by suspending the acid (4g) in H2O (400mL) at ~20o, heating to boiling with slow stirring, filtering hot and allowing to cool to ~20o. The acid is filtered off, washed with H2O, dried in air, recrystallised from 5% aqueous EtOH, washed well and dried over P2O5 in a moderate vacuum to constant weight. Recrystallisation from EtOH/EtOAc, and drying, gave the anhydrous acid. [Cortese & Bauman J Am Chem Soc 57 1393 1935, Bergstrom & Norman Acta Chem Scand 7 1126 1953, Beilstein 10 IV 2077.]
N-(3α,7α,12α-トリヒドロキシ-24-オキソ-5β-コラン-24-イル)グリシン 上流と下流の製品情報
原材料
準備製品