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Proteinase K

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Proteinase K manufacturers

  • Proteinase K
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  • 2024-03-28
  • CAS:39450-01-6
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Related articles

  • What is Proteinase K?
  • Proteinase K is a medium-sized globular α/β protein and widely used in molecular biology
  • Oct 23,2023
Proteinase K Basic information
Product Name:Proteinase K
Synonyms:ProteinaseK,PowderAndSolutionAvailable;ProteinaseK,fromTritirachiumalbumLimber;Proteinase K from Tritirachium album ca. 8 DMC-U/mg;PROTEINASEK,LYOPHILIZEDPOWDER,BIOTECHGRADE;Proteinase K for tritirachium album serine.;Proteinase K from Tritirachium album [EC 3.4.21.64] from Tritirachium album;proteinase k for tritirachium album;Proteinase K, tritirachium album serine
CAS:39450-01-6
MF:C29H27N2O12P
MW:626.504641
EINECS:254-457-8
Product Categories:Furans ,Coumarins;enzyme;39450-01-6
Mol File:39450-01-6.mol
Proteinase K Structure
Proteinase K Chemical Properties
storage temp. 2-8°C
solubility H2O: 10 mg/mL, slightly hazy, brown-yellow
form powder
color white
PHpH7.5~12
Water Solubility Miscible with water.
InChIKeyCDWGKFQEIBBJOF-BIZWTRPUNA-N
SMILESN12[C@](OP(O)(OC3=CC=C([N+]([O-])=O)C=C3)=O)([C@H]([C@H](O)C)C1=O)[C@](C)(OC1=CC=CC=C1)C(OC1=CC=CC=C1)=C2C(OC)=O |&1:1,16,17,22,r|
CAS DataBase Reference39450-01-6
Safety Information
Hazard Codes Xn
Risk Statements 36/37/38-42
Safety Statements 23-24-26-36/37-22-45
WGK Germany 1
10
HS Code 35079090
MSDS Information
ProviderLanguage
Prok English
SigmaAldrich English
Proteinase K Usage And Synthesis
DescriptionProteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in the absence of this divalent cation some catalytic activity is retained. Maximum proteinase K activity is observed with the inclusion of 1 mM Ca++ in the reaction buffer. Proteinase K digestion is routinely performed at 50°C, and occasionally in the presence of EDTA to inhibit labile, Mg++-dependent nucleases. Proteinase K is prepared commonly as a 20 mg/mL stock solution in sterile water (stable for 1 year at ?20°C) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl2 (stable for months at 4°C). It is generally used at a working concentration of up to 50 μg/mL in any of a number of buffer formulations, including those that contain as much as 0.5% SDS[1].
Chemical PropertiesPowder beige
UsesUseful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
UsesA stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.
DefinitionProteinase K cleaves peptide bonds next to the carboxyl-terminal of aromatic amino acids, hydrophobic amino acids and sulfuric amino acids, within the polypeptide chain. Hence Proteinase K can act as an endoprotease on a wide range of protein substrates. Because of its proteolytic preferences, it is a member of the subtilisin group of serine proteases. The K in Proteinase K refers to its ability to digest Keratin protein found in hairs, nails and hooves. Proteinase K is an essential reagent in molecular biology labs because it is useful in isolating DNA and RNA molecules by degrading and inactivating proteins. Fungus Tritirachium album Limber naturally produces proteinase K.
General DescriptionProteinase K, an extracellular endopeptidase is synthesized by the mold, Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein and is characterized with an unhydrolyzed protein chain and autolyzed polypeptide chains.
Biochem/physiol ActionsProteinase K catalyzes the hydrolysis of keratin.
References[1] Farrell, R. “Creating a Ribonuclease-Free Environment.”RNA Methodologies (Sixth Edition) 2023: 51-70.
Proteinase K Preparation Products And Raw materials
Tag:Proteinase K(39450-01-6) Related Product Information
Papain Trypsin EC 3.4.21.19 Pepsin Chymotrypsin ENTEROKINASE Aprotinin Proteinase K Solution DISPASE I EC 3.4.21.14,PROTEINASE 6 FROM ASPERGILLUS SP., ~3 UN ITS/MG EC 3.4.21.14,PROTEINASE, BACTERIAL, ~10 U/MG PROTEINASE FROM BACILLUS LICHENIFORMIS, ~8 U/MG,EC 3.4.21.14 Proteinase, Bacillus subtilis metallo- Acid resistant proteinase neutral proteinase PROTEINASE FROM ASPERGILLUS NIGER >4 U/M,EC 3.4.21.14 EC 3.4.21.14,EC 3.4.21.64 Proteinase, Bacillus subtilis, sutilain