β-Elemene ((-)-β-Elemene, Levo-β-elemene) 是一种从草本Curcuma Rhizoma中提取的倍半萜烯类化合物，具有抗肿瘤的活性。β-Elemene 可以抑制细胞增殖，阻止细胞周期，并诱导细胞凋亡或自噬。
β-Elemene (0-200 µg/ml; 24 hours) shows IC
50
values of 72.8 µg/ml; 47.4 µg/ml; 61.5 µg/ml; 3.661 µg/ml; 68 µg/ml; 72.12 µg/ml; 37.894 µg/ml and 37.703 µg/ml for SV-HUC-1, T24, 5637, TCCSUP, J82,UMUC-3,RT4, and SW780 cells, respectively.
β-Elemene (0-75 µg/ml; 24 hours) decreases cell number from 50 µg/ml and is notably decreased at 75 µg/ml, induces dose-dependent G1-phase arrest in T24cells and significantly reduces the percentage of cells in the S-phase .
β-Elemene (50 µg/ml; 12 hours) downregulates the expression levels of p-STAT3 and the cell cycle-related proteins cyclin D1, CDK4 and CDK6, and upregulates p21 and p27expression in T24 cells .
β-Elemene (50 µg/ml; 24 hours) enhances cisplatin-induced apoptosis by activating the ROS-AMPK signaling pathway.
Cell Viability Assay
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Cell Line:
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T24, 5637, TCCSUP, J82, UM-UC-3, RT4 and SW780 cells
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Concentration:
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0, 6.25, 12.5, 25, 50, 100, 150 and 200 µg/ml
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Incubation Time:
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24 hours
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Result:
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Inhibited the proliferation of RT4, SW780, J82, UMUC-3, TCCSUP, 5637 and T24 human bladder cancer cells and SV-HUC-1 human urothelial cells.
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Cell Cycle Analysis
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Cell Line:
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T24 and 5637 cells
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Concentration:
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0, 25, 50 and 75 µg/ml
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Incubation Time:
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24 hours
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Result:
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Induced the percentage of cells in the S-phase as a dose-dependent manner.
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Western Blot Analysis
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Cell Line:
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T24 cells
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Concentration:
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50 µg/ml
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Incubation Time:
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24 hours
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Result:
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Downregulated p-STAT3 and cell cycle-related proteins.
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