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J & K SCIENTIFIC LTD.
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Product Name:5-Cyano-2,3-ditolyl tetrazolium chloride CAS:90217-02-0 Package:50mg, 100mg
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Product Name: | CTC | Synonyms: | 5-Cyano-2,3-bis(4-methylphenyl)tetrazolium chloride;CTC;CYANODITOLYL TETRAZOLIUM CHLORIDE;CTC 5-Cyano-2,3-ditolyl tetrazolium chloride;5-CYANO-2,3-DI-(P-TOLYL)TETRAZOLIUM CHLORIDE;5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE;5-CYANO-2,3-BIS(4-METHYLPHENYL)-2H-TETRAZOLIUM CHLORIDE;2h-tetrazolium, 5-cyano-2,3-bis(4methylphenyl)-, Chloride | CAS: | 90217-02-0 | MF: | C16H14ClN5 | MW: | 311.77 | EINECS: | | Product Categories: | marker;Tetrazolium salt | Mol File: | 90217-02-0.mol | |
Melting point | 225 ℃ | storage temp. | 2-8°C | solubility | Soluble in water | form | powder | color | Off-white | λmax | 450nm (of formazan) | BRN | 8954451 | Biological Applications | NADPH-cytochrome P 450 reductase activity assays; detecting gamma-hydroxybutyric acid (GHB),metabolic activity,microorganisms; localizing glucose-6-phosphate dehydrogenase activity; monitoring respiratory activity; treating cancer |
Hazard Codes | Xi | Risk Statements | 36/37/38 | Safety Statements | 26 | WGK Germany | 3 | F | 8 | HS Code | 2933998090 |
Description | 5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable. | Uses | 5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells. | Uses | Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity. |
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