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| | HOECHST 34580 Basic information |
| Product Name: | HOECHST 34580 | | Synonyms: | HOECHST 34580;HOE 34580;ProaMine;2,50-bibenzimidazole, 20-[p-(dimethylamino)phenyl]-5-(4-methyl-1-piperazinyl)-;Benzenamine, N,N-dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-;Hoechst 34580 (HOE 34580);N,N-Dimethyl-4-(5-(4-methylpiperazin-1-yl)-1H,1'H-[2,5'-bibenzo[d]imidazol]-2'-yl)aniline | | CAS: | 23555-00-2 | | MF: | C27H29N7 | | MW: | 451.57 | | EINECS: | | | Product Categories: | Benzimidazole | | Mol File: | 23555-00-2.mol |  |
| | HOECHST 34580 Chemical Properties |
| Melting point | >300℃ | | Boiling point | 733.2±70.0 °C(Predicted) | | density | 1.280±0.06 g/cm3(Predicted) | | storage temp. | 2-8°C(protect from light) | | solubility | Soluble in dimethyl sulfoxide, water | | form | powder | | pka | 10.97±0.10, most acidic, temperature:
25 °C; 7.66 ± 0.42, most basic(at 25℃) | | color | Yellow to green | | λmax | 392nm | | Biological Applications | Detecting malaria infected red blood cells,polynucleotides; nucleic acid amplification; nucleic acid sequencing |
| | HOECHST 34580 Usage And Synthesis |
| Biological Activity | Hoechst 34580 (HOE 34580) is a cell-permeable blue fluorescent dye used to stain DNA and cell nuclei. It is also a good candidate for the treatment of Alzheimer's disease by inhibiting the formation of amyloid beta (Aβ) with an IC50 value of 0.86 μM. | | in vitro | Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC 50 of 0.86±0.05 μM for Hoechst 34580. It is a good candidate for treating the Alzheimer's disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose- dependent manner. And the half-maximal concentration (IC 50 ) is examined. The IC 50 is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86 ±0.05 μM for Hoechst 34580. | | target | | Target | Value | Aβ
(Cell-free assay) | 0.86 μM |
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| | HOECHST 34580 Preparation Products And Raw materials |
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