BH3I-1
| 中文名称 | BH3I-1 |
|---|---|
| 中文同义词 | BH3I-1游离态;化合物BH3I1;2-(5-(4-溴苯基亚甲基)-4-氧代-2-硫代噻唑-3-基)-3-甲基丁酸 |
| 英文名称 | BH3I-1 |
| 英文同义词 | 5-(p-Bromobenzylidine)-α-isopropyl-4-oxo-2-thioxo-3-thiozolidineacetic acid;BH3I 1 ;BH3I1;BHI1;BHI1;BH 3I1;3-Thiazolidineacetic acid, 5-[(4-bromophenyl)methylene]-α-(1-methylethyl)-4-oxo-2-thioxo-;5-(p-Bromobenzylidene)-a-isopropyl-4-oxo-2-thioxo-3-thiazolidine acetic acid;2-(5-(4-Bromobenzylidene)-4-oxo-2-thioxothiazolidin-3-yl)-3-methylbutanoic acid;MDM-2/p53,BH3I 1,Inhibitor,Ubiquitin ligase,BH3I-1,E3 ligating enzyme,Ubiquitin conjugating enzyme,BH3I1,E2 conjugating enzyme,Bcl-2 Family,BHI 1,E1 activating enzyme,inhibit,BHI-1,BH 3I1,E1/E2/E3 Enzyme,Ubiquitin activating enzyme,BH-3I-1 |
| CAS号 | 300817-68-9 |
| 分子式 | C15H14BrNO3S2 |
| 分子量 | 400.31 |
| EINECS号 | |
| 相关类别 | 标准品;API |
| Mol文件 | 300817-68-9.mol |
| 结构式 |  |
BH3I-1 性质
| 沸点 | 535.2±60.0 °C(Predicted) |
|---|---|
| 密度 | 1?+-.0.1 g/cm3(Predicted) |
| 储存条件 | Sealed in dry,2-8°C |
| 溶解度 | DMSO：>10 mg/mL |
| 形态 | 粉末，黄色 |
| 酸度系数(pKa) | 3.39±0.10(Predicted) |
|
Bcl-2
|
Bcl-xL
|
Bim
|
Bak
|
p53/mDM2 5.3 μM (Kd) |
BH3I-1, while inhibiting its reported target Bcl-2/Bim and Bcl-xL/Bim, shows significant inhibition of both the p53/hDM2 and p300/Hif-1α interactions. This surprising promiscuity, displays by a well studied compound leads to further interrogate the p53/hDM2 interaction utilizing a standard fluorescence polarization (FP) assay with purified protein. The results from the FP assay validates the split-luciferase screen and demonstrates that BH3I-1 has a K d =5.3 μM against the p53/mDM2 pair, which is comparable to its low micromolar potency reported for the BH3 family of receptors. BH3I-1 inhibits interaction between the BH3 domain and Bcl-xL. NMR analyses reveal that BH3I-1 targets the BH3-binding pocket of Bcl-xL with a K i of 7.8±0.9 μM.