108340-60-9
中文名称
灵芝酸D
英文名称
Ganoderic acid D
CAS
108340-60-9
分子式
C30H42O7
分子量
514.65
MOL 文件
108340-60-9.mol
更新日期
2024/04/25 13:34:07
108340-60-9 结构式
基本信息
中文别名
灵芝酸D灵芝酸D标准品
灵芝酸D对照品,
英文别名
(7beta)-7-Hydroxy-3,11,15,23-tetraoxolanost-8-en-26-oic acidLanost-8-en-26-oic acid, 7-hydroxy-3,11,15,23-tetraoxo-, (7β)-
所属类别
有机原料：羧酸类化合物及衍生物物理化学性质
外观性状白色粉末，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于多孔菌科灵芝Ganodermalucidum（Leyss.exFr.）Karst．的干燥子实体。
沸点688.3±55.0 °C(Predicted)
密度1.22±0.1 g/cm3(Predicted)
储存条件Store at -20°C, protect from light
溶解度Soluble in methanol;
酸度系数(pKa)4.77±0.23(Predicted)
形态粉末
颜色白色
水溶解性不溶于水
常见问题列表
生物活性
Ganoderic acid D 是一种高度氧化的四环三萜类化合物 (tetracyclic triterpenoid)，是灵芝 (Ganoderma lucidum) 的主要活性成分。Ganoderic acid D 上调 SIRT3 的蛋白质表达并通过 SIRT3 诱导脱乙酰化的亲环蛋白 D (CypD)。Ganoderic acid D 抑制结肠癌细胞的能量重编程，包括结肠癌细胞中的葡萄糖摄取，乳酸产生，丙酮酸和乙酰辅酶的产生。Ganoderic acid D 诱导 HeLa 人宫颈癌细胞凋亡 (apoptosis)。靶点
|
SIRT3
|
体外研究
Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC
50
of 17.3 mM.
Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells.
Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3.
Cell Viability Assay
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 1, 5, 10, 20, 50 μM |
| Incubation Time: | 24, 48, 72 hours |
| Result: | Reduced the cell survival rate in a dose- and time-dependent manner and had an IC 50 value of 17.3 μM for 48 hours treatment. |
Cell Cycle Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10, 50 μM |
| Incubation Time: | 24, 48 hours |
| Result: |
Induced G2/M phase arrest.
Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM. |
Apoptosis Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10, 50 μM |
| Incubation Time: | 48 hours |
| Result: | Induced a morphological change typical of apoptosis in HeLa cells. |
Western Blot Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10 μM |
| Incubation Time: | 48 hours |
| Result: | Up-regulated 14-3-3E and PRDX3. |