115534-33-3
中文名称
TMA-DPH(疏水膜荧光探针)
英文名称
TMA-DPH
CAS
115534-33-3
分子式
C28H31NO3S
分子量
461.62
MOL 文件
115534-33-3.mol
更新日期
2025/11/14 17:28:57
115534-33-3 结构式
基本信息
中文别名
膜电位荧光探针TMA-DPHTMA-DPH(疏水膜荧光探针)
英文别名
TMA-DPHTMA-DPH *CAS#: 115534-33-3*
4'-(Trimethylammonio)diphenylhexatrien
4'-(TRIMETHYLAMMONIO)DIPHENYLHEXATRIENE
1-(4-TRIMETHYLAMMONIUMPHENYL)-6-PHENYL-1 ,3,5-HEXAT
1,6-DIPHENYL-1,3,5-HEXATRIENE-4'-TRIMETHYLAMMONIUM TOSYLATE
4'-(Trimethylammonio)diphenylhexatriene p-toluenesulfonate
N,N,N-TRIMETHYL-4-[6-PHENYLHEXATRIEN-1-YL]PHENYLAMMONIUM TOSYLATE
1-(4-Trimethylammoniophenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate
1-(4-TRIMETHYLAMMONIUMPHENYL)-6-PHENYL-1,3,5-HEXATRIENE P-TOLUENESULFONATE
物理化学性质
熔点237 °C (dec.)(lit.)
储存条件−20°C
溶解度DMF: soluble
形态A crystalline solid
颜色Off-white to light brown
外观Solid Powder
常见问题列表
疏水膜荧光探针
TMA-DPH是一种疏水性荧光膜探针。TMA-DPH在测量膜流动性方面。它能够锚定在细胞表面，定位磷脂双分子层的不同区域，通过分析其在质膜及膜的亚结构中的荧光极化数值，可以测定细胞膜的流动性。因此，TMA-DPH特别适用于研究脂蛋白和类似系统的单层动力学。此外，它还可以用于荧光标记实验。
良好的灵敏度：TMA-DPH在脂质环境中能保持良好的稳定性和灵敏性，能够反映细胞膜的电位和流动性变化。
快速整合：当TMA-DPH与细胞接触时，它能迅速被整合到细胞膜中，实现快速检测。
动态追踪：在体外研究中，TMA-DPH能够追踪细胞膜的动态变化，如侧向扩散、旋转、左右摇摆等。
生物活性
TMA-DPH 是一种疏水性荧光膜探针 (Ex=355 nm; Em=430 nm)。体外研究
In contact with cells, TMA-DPH is instantaneously incorporated into the plasma membrane. TMA-DPH is concentrated in lysosomes and highly acidic late endosomes. The TMA-DPH fluorescence lifetime remains constant, 6.2±0.2 ns, below the same critical concentration of 2 μM, but decreases significantly with higher concentrations. This decrease, however, slows down above 5 μM. The TMA-DPH fluorescence anisotropy displays a similar evolution to the fluorescence lifetime. It is first constant, 0.283±0.003, again until 2 μM, and then decreases rapidly to 0.270±0.003 with 5 μM, continuing to fall, but at a lower rate with higher concentrations.