171783-05-4
中文名称
171783-05-4
CAS
171783-05-4
分子式
C48H77N17O17
分子量
1164.23
MOL 文件
171783-05-4.mol
更新日期
2024/05/05 20:58:33
171783-05-4 结构式
基本信息
英文别名
Crosstide >=97% (HPLC), lyophilized powderGlycine, glycyl-L-arginyl-L-prolyl-L-arginyl-L-threonyl-L-seryl-L-seryl-L-phenylalanyl-L-alanyl-L-α-glutamyl-
Glycine, glycyl-L-arginyl-L-prolyl-L-arginyl-L-threonyl-L-seryl-L-seryl-L-phenylalanyl-L-alanyl-L-a-glutaMyl-
常见问题列表
生物活性
Crosstide 是一种糖原合成酶激酶α/β融合蛋白序列的模拟肽，是 Akt 的底物。靶点
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Akt
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体外研究
16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme.