2447-54-3
基本信息
血根碱
血根碱40%
假白屈菜季铵碱
血根碱(标准品)
血根碱.博落回提取物
血根碱(假白屈菜季铵碱)
SANGUINARINE 血根碱
Sangrovit
SANGUINARIN
SANGUINARINE
PSEUDOCHELERYTHRINE
SANGUINARINE, 40+% BY UV
SANGUINARINE, 98+% BY HPLC
dimethylenedioxybenzphenanthridine
Sanguinarine/Macleaya cordata extract
SANGUINARINE CHLORIDE ISOLATED FROM MACL EAY
物理化学性质
常见问题列表
图1为血根碱化学结构式
临床研究结果表明,血根碱对口腔中大多数细菌有抑菌和杀菌作用,并有干扰细菌粘附于刚形成的牙膜上的作用。含血根碱提取物(SaE)/氯化锌的牙膏和漱口液的临床试验表明,用含质量分数为0.075%SaE、质量分数为2.0%或更多的氯化锌牙膏和含质量分数为0.03%SaE、质量分数为0.2%氯化锌漱口液进行试验,7位试验者牙菌斑全部减少,牙龈健康改善,其中3位经历6个月后见效。短期试验的效果还有待进一步证实。
血根碱作用机理还需要进一步探讨。氯化锌和血根碱各自对复配物活性的贡献还不清楚,市售含血根碱的牙膏是含有阳离子抗菌剂和阴离子表面活性剂(月桂基硫酸酯钠盐) 的配方,在该牙膏中血根碱(阳离子抗菌剂)仍然保持其抗菌活性,其原因还不清楚。
以上信息由Chemicalbook彤彤编辑整理。
Apoptosis
Sanguinarine (SANG)-induced apoptosis is associated with the activation of JNK and NF-κB signal pathways.To determine the effects of Sanguinarine on cell viability, 22B-cFluc cells are stimulated with different concentrations of Sanguinarine for 24 h, and then a CKK-8 assay is performed. The treatment with Sanguinarine decreases the proliferation of 22B cells in a dose-dependent manner. Meanwhile, the cytosolic extracts of 22B-cFluc cells treated with different dose of Sanguinarine are measured to detect cellular caspase-3 activity using Ac-DEVD-pNA, which is a validated caspase-3 substrate. The absorbance at 450 nm increases in a dose-dependent manner, indicating increased caspase-3 activity stimulated by Sanguinarine.
To evaluate the apoptosis induced by Sanguinarine (SANG) in vivo, 22B-cFluc cells are inoculated subcutaneously into one flank of nude mice and xenograft models are allowed to establish. Mice are treated intravenously with 10 mg/kg of Sanguinarine. At 24, 48 and 72 h after treatment, bioluminescent imaging is performed after i.p. injection of mice with 150 mg/kg of D-luciferin substrate. Sanguinarine treatment induces an obvious increase of luminescent signal as early as 48 h after initial treatment. A sustained bioluminescent imaging (BLI) intensity increased is observed throughout the course of experiment. At 72 h after treatment, the tumors are collected and subjected to TUNEL staining for evaluating apoptosis. Compared with the control tumors, the group treated with Sanguinarine exhibits significantly more cell apoptosis, indicated by the increased green signals from the sporadic apoptotic cells.