3148-09-2
3148-09-2 结构式
基本信息
中文别名
疣孢菌素疣孢菌素A
英文别名
veraVerrucarin
NSC 126728
VERRUCARIN A
MUCONOMYCIN A
Muconomycin A, NSC 126728
16a,21-trimethyl--stereoisomer
verrucarin A from myrothecium sp
n-17(18h),2’-oxirane)-3,9,14-trione,4,5,6,7,16,16a,19a,22-octahydro-4-hydroxy
spiro(16,18-methano-1h,3h,23h-(1,6,12)trioxacyclooctadecino(3,4-d)(1)benzopyra
物理化学性质
熔点>360° (dec)
比旋光度D23 +206° (c = 1.012 in chloroform)
沸点512.66°C (rough estimate)
密度1.1922 (rough estimate)
折射率1.5500 (estimate)
储存条件−20°C
溶解度Dichloromethane: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble
酸度系数(pKa)12.76±0.70(Predicted)
形态固体
安全数据
警示词危险
危险性描述H300+H310+H330
危险品标志T+
危险类别码26/27/28
安全说明22-36/37/39-45
危险品运输编号UN 2811 6.1/PG 1
WGK Germany3
RTECS号WH1314900
危险等级6.1(a)
包装类别I
海关编码2941900000
毒性LD50 in mice, rats, rabbits (mg/kg): 1.5, 0.87, 0.54 i.v. (Rüsch, Sthlin)
常见问题列表
生物活性
Verrucarin A (Muconomycin A) 是一种 D 型大环真菌毒素，来源于Myrothecium verrucaria，是一种蛋白质合成 (protein synthesis) 的抑制剂。Verrucarin A 抑制白血病细胞系生长，激活巨噬细胞 caspases、凋亡和炎症信号。Verrucarin A 能有效提高 p38 MAPK 的磷酸化，降低 ERK/Akt 的磷酸化。Verrucarin A 通过 p21 和 p53 的诱导引起细胞周期调控的解除。体外研究
Verrucarin A (0-0.6 μM/ml; 24-48 hours)-induces time- and dose-dependent growth inhibition in MCF-7 cells.
Verrucarin A increases the levels of reactive oxygen species (ROS), and subsequently induces mitochondrial membrane potential (Δψm) loss, leading to the increase of Bax/Bcl-2 ratio, cytochrome c release, caspase activation, PARP degradation, and apoptosis.
Cell Viability Assay
| Cell Line: | MCF-7 cells |
| Concentration: | 0-0.6 μM/ml |
| Incubation Time: | 24 and 48 hours |
| Result: | Growth of MCF-7 cells is significantly inhibited in a dose- and time-dependent manner, with the IC 50 s of 0.41 and 0.29 μM/ml for 24- and 48-h treatment periods, respectively. |