CA-5F
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- CAS号:
- 1370032-19-1
- 英文名:
- CA-5f
- 英文别名:
- CA-5f;CA-5F;CA 5F;CA5F;1370032-19-1;-5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-;CA-5f,Apoptosis,inhibit,Autophagy,Inhibitor,CA 5f,CA5f;(3E,5E)-3-(3,4-dimethoxybenzylidene)-5-[(1H-indol-3-yl)methylene]-1-methylpiperidin-4-one;(E)-3-((1H-Indol-3-yl)methylene)-5-((E)-3,4-dimethoxybenzylidene)-1-methylpiperidin-4-one;4-Piperidinone, 3-[(3,4-dimethoxyphenyl)methylene]-5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-
- 中文名:
- CA-5F
- 中文别名:
- 化合物CA-5F;(E)-3-((1H-吲哚-3-基)亚甲基)-5-((E)-3,4-二甲氧基亚)-1-甲基哌啶-4-酮
- CBNumber:
- CB04808619
- 分子式:
- C24H24N2O3
- 分子量:
- 388.46
- MOL File:
- 1370032-19-1.mol
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CA-5F化学性质
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沸点:
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639.7±55.0 °C(Predicted)
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密度:
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1.253±0.06 g/cm3(Predicted)
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储存条件:
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Store at -20°C
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溶解度:
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DMSO : 77.5 mg/mL (199.51 mM);Water : < 0.1 mg/mL (insoluble)
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酸度系数(pKa):
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16.06±0.30(Predicted)
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CA-5F性质、用途与生产工艺
CA-5f 是一种有效的晚期巨自噬/自噬 (macroautophagy/autophagy) 抑制剂，通过抑制自噬体-溶酶体融合起作用。CA-5f 能够增加 LC3B-II (一种监控自噬的标记物) 和 SQSTM1 蛋白水平，促进 ROS 的产生。具有抗肿瘤活性。
CA-5f (0-40 μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs.
CA-5f (20 μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1 (100 nM) or Chloroquine (30 μM) in A549 cells and HUVECs.
CA-5f (20 μM) neither impairs the hydrolytic function nor the quantity of lysosomes.
CA-5f (20 μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs.
Cell Viability Assay
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Cell Line:
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A549, HUVECs
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Concentration:
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20 μM
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Incubation Time:
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96 hours
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Result:
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Exhibited more cytotoxicity against A549 cells compared with normal HUVECs.
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Western Blot Analysis
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Cell Line:
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A549 cells and HUVECs
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Concentration:
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0-40 μM
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Incubation Time:
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6 hours
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Result:
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Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner.
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CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells.
CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells.
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Animal Model:
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Nude mice bearing A549 lung cancer cells
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Dosage:
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40 mg/kg
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Administration:
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Injected via caudal vein, every 2 days for up to 30 days
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Result:
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Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice.
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CA-5F
上下游产品信息
上游原料
下游产品
| 更新日期 | 产品编号 | 产品名称 | CAS编号 | 包装 | 价格 |
|---|
| 2024/04/30 | HY-112698 | CA-5f | | 1 mg | 419元 |
| 2024/04/30 | HY-112698 | CA-5F
CA-5f | 1370032-19-1 | 5mg | 1100元 |
1370032-19-1, CA-5F 相关搜索:
- API
- 药靶配体
- 抑制剂
- 对照品
- (E)-3-((1H-吲哚-3-基)亚甲基)-5-((E)-3,4-二甲氧基亚)-1-甲基哌啶-4-酮
- 化合物CA-5F
- 1370032-19-1
- (3E,5E)-3-(3,4-dimethoxybenzylidene)-5-[(1H-indol-3-yl)methylene]-1-methylpiperidin-4-one
- CA-5f,Apoptosis,inhibit,Autophagy,Inhibitor,CA 5f,CA5f
- (E)-3-((1H-Indol-3-yl)methylene)-5-((E)-3,4-dimethoxybenzylidene)-1-methylpiperidin-4-one
- -5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-
- 4-Piperidinone, 3-[(3,4-dimethoxyphenyl)methylene]-5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-
- CA-5F;CA 5F;CA5F;1370032-19-1
- CA-5f