ANTI-BAD

ANTI-BAD性质、用途与生产工艺

描述

BAD Polyclonal Antibody

宿主/亚型

Rabbit / IgG

种属反应

Non-human primate, Rat, Human, Mouse

偶联物

Unconjugated

验证的应用

IP WB

品牌示例

Thermofisher BAD Antibody (PA5-17896) in WB

Western blot analysis of BAD was performed with 10 µg of A549 cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against BAD (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (Product # 37539) for 1 hour at room temperature. BAD was detected at ~ 23 kDa using BAD Rabbit polyclonal antibody (Product # PA5-17896) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Rabbit (Product # 31461) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to GAPDH (36 kDa). BAD Antibody (Product # PA5-17896) confirms silencing of BAD expression.

背景

The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

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ANTI-BAD 相关搜索:

• Clone Index
• Cell Biology
• BAntibodies
• Antibodies to Bcl-2 Family ProteinsAntibodies
• Antibodies for Apoptosis
• Alphabetical Index
• 6
• 抗体-一抗
• ANTI-BAD:相关死亡促进因子BAD抗体
• ANTI-BAD
• ANTI-MOUSE BAD
• Anti-BCL2L8
• Anti-BCL2-associated agonist of cell death
• Anti-BBC2
• Monoclonal Anti-BAD antibody produced in mouse
• Anti-BCL2-antagonist of cell death
• Anti-BAD antibody produced in chicken
• Anti-Bcl-2-like 8
• Monoclonal Anti-Bad antibody produced in rat
• MONOCLONAL ANTI-BAD
• ANTI-BAD, HUMAN (MOUSE)
• ANTI-BAD (HUMAN)
• ANTI-BAD(H-168)
• ANTI-BCL2L8 (HUMAN)
• ANTI-HUMAN/MOUSE BCL-2 ANTAGONIST OF CELL DEATH AA 1-21
• ANTI-HUMAN/MOUSE BAD AA 1-21