6-丙烯酰基-2-二甲氨基萘
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- CAS号:
- 86636-92-2
- 英文名:
- 6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE
- 英文别名:
- ACRYLODAN;6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE;1-(6-Dimethylamino-naphthalen-2-yl)-propenone;Acrylodan [6-Acryloyl-2-dimethylaminophthalene];ACRYLODAN [6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE];1-(6-(Dimethylamino)-2-naphthalenyl)-2-propen-1-one;1-(6-(Dimethylamino)naphthalen-2-yl)prop-2-en-1-one;2-Propen-1-one, 1-(6-(dimethylamino)-2-naphthalenyl)-
- 中文名:
- 6-丙烯酰基-2-二甲氨基萘
- 中文别名:
- 6-丙烯酰-2-二甲氨基萘;6-丙烯酰基-2-二甲氨基萘;1-(6-(二甲基氨基)萘-2-基)丙-2-烯-1-酮
- CBNumber:
- CB4347798
- 分子式:
- C15H15NO
- 分子量:
- 225.29
- MOL File:
- 86636-92-2.mol
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6-丙烯酰基-2-二甲氨基萘化学性质
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熔点:
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130-131 °C(Solv: ethanol (64-17-5); water (7732-18-5))
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沸点:
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388.3±24.0 °C(Predicted)
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密度:
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1.100±0.06 g/cm3(Predicted)
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储存条件:
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2-8°C
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形态:
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Solid
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酸度系数(pKa):
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4.32±0.40(Predicted)
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颜色:
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Light yellow to yellow
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6-丙烯酰基-2-二甲氨基萘性质、用途与生产工艺
Acrylodan 可与硫醇反应,对 Cys34 周围的结合口袋内的局部环境双极性和动力学敏感。
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M
−1
cm
−1
for actin at 290 nm, 18,500 M
−1
cm
−1
or acrylodan at 385 nm, and 71,000 M
−1
cm
−1
for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 (
Acryl
Act1
NAT
) is dramatically different than that of EDTA-unfolded
Acryl
Act1 (
Acryl
Act1I3), which has a very similar emission spectrum to CCT-bound
Acryl
Act1.
6-丙烯酰基-2-二甲氨基萘
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更新日期 | 产品编号 | 产品名称 | CAS编号 | 包装 | 价格 |
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2024/08/19 | HY-W040230 | 6-丙烯酰基-2-二甲氨基萘 Acrylodan | 86636-92-2 | 5mg | 800元 |
2024/08/19 | HY-W040230 | Acrylodan | | 10 mg | 1240元 |
86636-92-2, 6-丙烯酰基-2-二甲氨基萘 相关搜索:
- 6-丙烯酰-2-二甲氨基萘
- 1-(6-(二甲基氨基)萘-2-基)丙-2-烯-1-酮
- 6-丙烯酰基-2-二甲氨基萘
- 86636-92-2
- Acrylodan [6-Acryloyl-2-dimethylaminophthalene]
- 1-(6-(Dimethylamino)-2-naphthalenyl)-2-propen-1-one
- ACRYLODAN [6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE]
- 6-ACRYLOYL-2-DIMETHYLAMINONAPHTHALENE
- ACRYLODAN
- 1-(6-Dimethylamino-naphthalen-2-yl)-propenone
- 1-(6-(Dimethylamino)naphthalen-2-yl)prop-2-en-1-one
- 2-Propen-1-one, 1-(6-(dimethylamino)-2-naphthalenyl)-