Tetrac (0.01-1 μM; 2-6 d) induces anti-proliferation in HT-29 and HCT116 cells with different K-RAS status. Tetrac (0.1 μM; 30 min) inhibits activation of ERK1/2 in HT-29 and HCT116 cells. Tetrac (0.1 μM; 24 h) inhibits expression of CCND1 and c-Myc , but promotes expression of CASP2 and THBS1 .
Cell Proliferation Assay
Western Blot Analysis
Tetrac (35 μg; p.o. for 40 days) inhibits tumor inoculation, growth and integrin expression in mice.