Ziyuglycoside II (10-60 μM; 24 h and 48 h) inhibits MDA-MB-435 cells growth in a dose-dependent manner. The IC 50 of Ziyuglycoside II at 24 h and 48 h is 5.92 μM and 4.74 μM, respectively. Ziyuglycoside II (5-25 μM) induces G0/G1 and S phase arrest in MDA-MB-435 cells at 24 h. Ziyuglycoside II (5-25 μM; 24 hours) significantly increases apoptotic rate of MDA-MB-435 cells. Ziyuglycoside II (5-25 μM; 24 hours) increases expressions of both p53 and p21 in MDA-MB-435 cells, which effect is dose-dependent.
Cell Viability Assay
Cell Cycle Analysis
Apoptosis Analysis
Western Blot Analysis