2,7-双(2-羧乙基)-5(6)-羧基荧光素
|
|
- CAS号:
- 85138-49-4
- 英文名:
- 2`,7`-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
- 英文别名:
- BCECF;BCECF acid *CAS#: 85138-49-4*;2',7'-Biscarboxyethyl-6-carboxyfluorescein;2',7'-BIS-(CARBOXYETHYL)-CARBOXY-FLUORESCEIN;2,7-Bis(2-carboxyethyl)-6-carboxyfluoresceine;2',7'-Bis(carboxyethyl)-4or5-carboxyfluorescein;2'7'-BIS-(2-CARBOXYETHYL)-5-(AND-6) FLUORESCEIN;2',7'-BIS(CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN;2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN;2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN
- 中文名:
- 2,7-双(2-羧乙基)-5(6)-羧基荧光素
- 中文别名:
- PH荧光探针BCECF,酸;2,7-双-(羧乙基)-羧基-荧光素;2,7-双(2-羧乙基)-5(6)-羧基荧光素;2`,7`-双(2-羧基乙基)-5(6)-羧基荧光素;2',7'-二-(2-羧乙基)-5(6)-羧基荧光素;5(OR 6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸;5(OR6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1CHEMICALBOOK(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸
- CBNumber:
- CB6347691
- 分子式:
- C27H20O11
- 分子量:
- 520.44
- MOL File:
- 85138-49-4.mol
|
|
|
2,7-双(2-羧乙基)-5(6)-羧基荧光素化学性质
-
储存条件:
-
-20°C
-
|
-
溶解度:
-
DMF: 5 mg/ml; DMSO: 15 mg/ml; DMSO:PBS (pH 7.2) (1:2): 0.25 mg/ml; Ethanol: 5 mg/ml
-
|
-
形态:
-
A crystalline solid
-
|
-
BRN:
-
8375770
-
|
2,7-双(2-羧乙基)-5(6)-羧基荧光素性质、用途与生产工艺
BCECF 是一种对 pH 敏感 (pH-sensitive) 的荧光染料,可用于监测细胞的 pH 比值。BCECF 允许测量生理 pH 值范围在 6.0-8.0 之间。激发波长: 490/440 nm; 发射强度: 535 nm。
Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K
+
/H
+
ionophor nigericin does not inhibit hydrogenosomal loading with BCECF.The pH-sensitive fluorescent dyes to measure cytosolic pH.
1.Prepare a 2 to 20 mM stock solution of BCECF in DMSO.
2.Prepare a 5-50 µM BCECF dye-loading solution in buffer solutions (HHBS or PBS).3. Add 1000 µL/well (6-well plate),100 µL/well (96-well plate) or 25 µL/well (384-well plate) BCECF dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at E
x
/E
m
= 490/535 nm or 430/535 nm for ratio measurements.
2,7-双(2-羧乙基)-5(6)-羧基荧光素
上下游产品信息
上游原料
下游产品
更新日期 | 产品编号 | 产品名称 | CAS编号 | 包装 | 价格 |
---|
2024/01/25 | HY-101882 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素 BCECF | 85138-49-4 | 1mg | 1600元 |
2,7-双(2-羧乙基)-5(6)-羧基荧光素
生产厂家
85138-49-4, 2,7-双(2-羧乙基)-5(6)-羧基荧光素 相关搜索:
- 荧光探针,标记,颗粒和染色
- 生化试剂
- Ion Probes
- Ion Channels
- Monovalent Ion Channels
- Detection
- Cell Signaling and Neuroscience
- Cell Biology
- BioChemical
- Biochemicals and Reagents
- Fluorescent Probes, Labels, Particles and Stains
- Fluorescent Labeled Polymers
- Fluorescent Conjugates
- Voltage-gated Ion Channels
- C27H20O11
- 5(OR6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1CHEMICALBOOK(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸
- PH荧光探针BCECF,酸
- 2`,7`-双(2-羧基乙基)-5(6)-羧基荧光素
- 2',7'-二-(2-羧乙基)-5(6)-羧基荧光素
- 5(OR 6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸
- 2,7-双-(羧乙基)-羧基-荧光素
- 2,7-双(2-羧乙基)-5(6)-羧基荧光素
- 85138-49-4
- BCECF acid *CAS#: 85138-49-4*
- 5(or 6)-Carboxy-3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9''-[9H]xanthene]-2',7'-dipropanoic acid
- 2',7'-bis(2-carboxyethyl)-3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
- BCECF acid [2',7-Bis-(2-Carboxyethyl)-5(6)-carboxyfluorescein]
- BCECF, 2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein
- 2',7'-Biscarboxyethyl-6-carboxyfluorescein
- 2,7-Bis(2-carboxyethyl)-6-carboxyfluoresceine
- 2',7'-Bis(2-carboxyethyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid
- 2',7'-BIS-(CARBOXYETHYL)-CARBOXY-FLUORESCEIN
- 2',7'-BIS(CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN
- 2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN
- 2'7'-BIS-(2-CARBOXYETHYL)-5-(AND-6) FLUORESCEIN
- 2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN
- 2'',7''-BIS(2-CARBOXYETHYL)-5(6)- CARBOXYFLUORESCEIN (BCECF)
- 2'',7''-Bis-(2-carboxyethyl)-4(5)-carboxyfluorescein
- 2',7'-Bis(carboxyethyl)-4or5-carboxyfluorescein
- 2',7'-bis(Carboxyethyl)-5(6)-Carboxyfluorescein (B
- 2',7'-bis(Carboxyethyl)-5(6)-Carboxyfluorescein(BCECF)
- BCECF