溴百里酚蓝 MSDS3',3"-Dibromothymolsulfonephthalein
溴百里酚蓝呈无色或浅玫瑰结晶性粉末状,易溶于醇、稀碱溶液和氨水中,微溶于水,不溶于石油醚。其遇酸性时为黄色,碱性时为蓝色。
溴麝香草酚蓝是一种酸碱指示剂,变色范围pH6.0(黄)~7.6(蓝)。普通水是中性,pH也就是7左右,差不多呈浅绿。
溴百里酚蓝是弱酸和弱碱的pH指示剂,在分析化学中用作酸碱指示剂和色谱分析试剂;也可用作染料和代谢产物。
• pH指示剂 — 在近中性时具有精确的颜色转换,酸性时为黄色,碱性时为蓝色。
• 染色剂 — 在显微镜下追踪淋巴液的运动,确定细胞壁或细胞核
• 培养基组分 — 在琼脂凝胶培养基中,用于蜡样芽孢杆菌计数;作为细菌生长组分和检测底物,例如胱氨酸/乳糖/电解质缺乏
• BTB纸片法 — 应急测定全血胆碱酯酶活力,用于有机磷中毒的判断。
方法1:在搅拌下,在百里酚蓝的冰醋酸悬浮液中通入溴,反应在50℃下进行,可制得溴百里酚蓝。
方法2:在搅拌下将过量的溴、冰醋酸溶液慢慢加到百里酚蓝的水醋酸溶液中,于40~50℃下反应4h反应结束后,冷却至20℃以下,过滤,干燥即得无色溴百里酚蓝。
A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies.