GLUTATHIONE S-TRANSFERASE Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Glutathione S-Transferase from human placenta has been used in antigen microarray and informatics analysis.
Allgemeine Beschreibung
Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene.
läuterung methode
It is purified by affinity chromatography using a column prepared by coupling glutathione to epoxy-saturated Sepharose. After washing contaminating proteins, the pure transferase is eluted with buffer containing reduced glutathione. The solution is then concentrated by ultrafiltration, dialysed against phosphate buffer at pH ~7 and stored in the presence of dithiothreitol (2mM) in aliquots at <-20o. [Simons & Vander Jag Anal Biochem 52 334 1977.]
GLUTATHIONE S-TRANSFERASE Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
