3-Hydroxybutyrate Dehydrogenase (3-HBDH) Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.
Allgemeine Beschreibung
3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from
Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.
Biochem/physiol Actions
3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3--hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.
3-Hydroxybutyrate Dehydrogenase (3-HBDH) Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
