INT/BCIP Stock Solution Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
INT/BCIP Stock Solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, that includes:
- Southern blot
- Western blot
- immunohistochemistry and immunocytochemistry
It has been used for colorogenic
in situ hybridization.
Allgemeine Beschreibung
BCIP (5-bromo-4-chloro-3-indoyl phosphate) is the AP (alkaline phosphatase)-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT (iodonitrotetrazolium) is the oxidant producing a red dye. The reaction product has a reddish-brown color and is insoluble in water.
INT/BCIP Stock Solution Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
