5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I

CAS-Nr.

Englisch Name:: 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I

Synonyma:: 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I

CBNumber:: CB34668968

Summenformel:

Molgewicht:: 0

MOL-Datei:: Mol file

Request For Quotation

Eigenschaften Sicherheit Verwendungswort Produktionsfirma 1

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I Eigenschaften

storage temp.: -20°C

Sicherheit

Risiko- und Sicherheitserklärung
Gefahreninformationscode (GHS)

Bildanzeige (GHS)

Alarmwort

Warnung

Gefahrenhinweise

Code	Gefahrenhinweise	Gefahrenklasse	Abteilung	Alarmwort	Symbol	P-Code
H315	Verursacht Hautreizungen.	Hautreizung	Kategorie 2	Warnung	src="/GHS07.jpg" width="20" height="20" />	P264, P280, P302+P352, P321,P332+P313, P362
H317	Kann allergische Hautreaktionen verursachen.	Sensibilisierung der Haut	Kategorie 1A	Warnung	src="/GHS07.jpg" width="20" height="20" />	P261, P272, P280, P302+P352,P333+P313, P321, P363, P501
H319	Verursacht schwere Augenreizung.	Schwere Augenreizung	Kategorie 2	Warnung	src="/GHS07.jpg" width="20" height="20" />	P264, P280, P305+P351+P338,P337+P313P
H412	Schädlich für Wasserorganismen, mit langfristiger Wirkung.	Langfristig (chronisch) gewässergefährdend	Kategorie 3			P273, P501

Sicherheit

P261	Einatmen von Staub vermeiden.
P264	Nach Gebrauch gründlich waschen.
P264	Nach Gebrauch gründlich waschen.
P273	Freisetzung in die Umwelt vermeiden.
P280	Schutzhandschuhe/Schutzkleidung/Augenschutz tragen.
P333+P313	Bei Hautreizung oder -ausschlag: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen.
P337+P313	Bei anhaltender Augenreizung: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen.

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU. The antibody to BrdU supplied with the kit contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments, which allow binding of the antibody to BrdU without destruction of the cellular morphology. A fluorescein-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. Subsequently, the sample is evaluated using an immunofluorescence microscope.

Safe: No radioisotopes are used
Easy to perform: Follows a standard immunofluorescence protocol
Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU
Flexible: Allows double-labeling protocols

BrdU Labeling and Detection Kit has been used for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.

Allgemeine Beschreibung

The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.
Normally, binding of the antibody is only achieved by denaturation of the DNA. This is usually obtained by exposing the cells to acid, base, or heat. These procedures result in destruction of cell integrity, including cell morphology and surface and cytoplasmatic markers.
The BrdU Labeling and Detection Kit I avoids these problems. The antibody preparation contains specific nucleases which allows access to BrdU after fixation in acidic ethanol. Therefore also simultaneous detection of other markers (double staining) is possible.

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 1)Lieferanten

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80