GC-RICH PCR System
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- CAS-Nr.
- Englisch Name:
- GC-RICH PCR System
- Synonyma:
- GC-RICH PCR System
- CBNumber:
- CB34669985
- Summenformel:
- Molgewicht:
- 0
- MOL-Datei:
- Mol file
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GC-RICH PCR System Eigenschaften
Sicherheit
- Risiko- und Sicherheitserklärung
- Gefahreninformationscode (GHS)
| Bildanzeige (GHS) |
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| Alarmwort |
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| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H412 |
Schädlich für Wasserorganismen, mit langfristiger Wirkung. |
Langfristig (chronisch) gewässergefährdend |
Kategorie 3 |
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P273, P501 |
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| Sicherheit |
| P273 |
Freisetzung in die Umwelt vermeiden. |
| P501 |
Inhalt/Behälter ... (Entsorgungsvorschriften vom Hersteller anzugeben) zuführen. |
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GC-RICH PCR System Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
The GC-RICH PCR System, a blend of Taq DNA polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superb performance – especially from problematic templates.
The GC-RICH PCR System may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA polymerase alone.
Advantages of the GC-Rich PCR System:
- Ease access to difficult templates, including GC-rich targets and repetitive sequences.
- Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
- Amplify DNA fragments up to 5 kb.
Allgemeine Beschreibung
The GC-RICH PCR System is composed of an enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR Reaction buffer in combination with the separately included GC-RICH resolution solution allows to amplify difficult templates like GC-rich targets very efficiently. TA cloning is recommended. The enzyme blend produces more blunt-ended fragments than Taq DNA Polymerase. The majority of products have single A overhangs. Use 2 U for a standard 50 μl PCR.
Contents- GC-RICH Enzyme Mix , in storage buffer
- GC-RICH Reaction Buffer, 5x concentrated, with 7.5 mM MgCl2 and DMSO
- GC-RICH Resolution Solution, 5 M
- MgCl2 Stock Solution, 25 mM
- Water, PCR Grade
GC-RICH PCR System Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
GC-RICH PCR System Anbieter Lieferant Produzent Hersteller Vertrieb Händler.
Global( 17)Lieferanten
