KAPA2G Fast HotStart PCR Kit

KAPA2G Fast HotStart PCR Kit Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

KAPA2G Fast HotStart PCR Kit has been used for:

• Fast PCR
• Genotyping
• The quantification of chromatin immunoprecipitation (ChIP)-seq libraries
• Routine polymerase chain reaction (PCR)

Allgemeine Beschreibung

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets. KAPA2G Fast HotStart^? PCR Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

Biochem/physiol Actions

KAPA2G Fast DNA Polymerase generates DNA fragments, which share the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase. It is useful for routine downstream analyses, like restriction enzyme digestion, cloning and sequencing. Similar to wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities. But it has no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is comparable to wild-type Taq, which has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.

KAPA2G Fast HotStart PCR Kit Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

KAPA2G Fast HotStart PCR Kit Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80

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• KAPA2G Fast HotStart PCR Kit