ANTI-NEOMYCIN PHOSPHOTRANSFERASE II Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Anti-NPTII antibody has been used in immunoblotting.
Allgemeine Beschreibung
Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the
E. coli transposon Tn5.
Biochem/physiol Actions
The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.
ANTI-NEOMYCIN PHOSPHOTRANSFERASE II Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
