Dehydrogenase, Glycerinaldehydphosphat
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- CAS-Nr.
- 9001-50-7
- Bezeichnung:
- Dehydrogenase, Glycerinaldehydphosphat
- Englisch Name:
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
- Synonyma:
- gapd;cb609;GAPDH;G-3-PDH;mg:bb02e05;EC: 1.2.1.12;EC 1.2.1.1.2;GAPDH)/CH8530;GAPDH from rabbit muscle;D-Glyceraldehyde 3-phosphate
- CBNumber:
- CB6673129
- Summenformel:
- C3H7O6P
- Molgewicht:
- 170.057841
- MOL-Datei:
- 9001-50-7.mol
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Dehydrogenase, Glycerinaldehydphosphat Eigenschaften
Sicherheit
- Risiko- und Sicherheitserklärung
- Gefahreninformationscode (GHS)
| Bildanzeige (GHS) |
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| Alarmwort |
Warnung |
| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H319 |
Verursacht schwere Augenreizung. |
Schwere Augenreizung |
Kategorie 2 |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
P264, P280, P305+P351+P338,P337+P313P |
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| Sicherheit |
| P264 |
Nach Gebrauch gründlich waschen. |
| P264 |
Nach Gebrauch gründlich waschen. |
| P280 |
Schutzhandschuhe/Schutzkleidung/Augenschutz tragen. |
| P305+P351+P338 |
BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
| P337+P313 |
Bei anhaltender Augenreizung: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen. |
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Dehydrogenase, Glycerinaldehydphosphat Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
- for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
- to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
- in anti-aggregation assays
- in purified GAPDH studies
Allgemeine Beschreibung
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.
läuterung methode
Purify the dehydrogenase from rabbit muscle by extraction with 0.03N KOH and precipitate it with (NH4)2SO4 (0.52 of saturation). The clear supernatant is adjusted to pH 7.5, and NH3 is added dropwise to pH 8.2-8.4. Crystals appear sometimes even without seeding. The crystals are dissolved in H2O, filtered to remove suspended material and 2 volumes of saturated (NH4)2SO4 at pH 8.2-8.4 is added. After 1hour the crystals appear. Recrystallise it in the same way. [Cori et al. J Biol Chem 173 605 1948, Furfine & Velick J Biol Chem 240 844 1965, The Enzymes 7 243 1963, Lui & Huskey Biochemistry 31 6998 1992.] The Km values are: NADH (3.3WM) and 1,3-diphosphoglycerate (8x10-7M) in pH 7.4 imidazole buffer at 26o, NAD (13WM), glyceraldehyde-3-P (90WM), Pi (2.9x10-4M), and arsenate (69WM) in 8.6 M NaHCO3 buffer at 26oC. [Orsi & Cleland Biochemistry 11 102 1972.]
Dehydrogenase, Glycerinaldehydphosphat Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
Dehydrogenase, Glycerinaldehydphosphat Anbieter Lieferant Produzent Hersteller Vertrieb Händler.
Global( 114)Lieferanten
9001-50-7(Dehydrogenase, Glycerinaldehydphosphat)Verwandte Suche:
Tris(pentan-2,4-dionato)eisen(II)
1,1,3,3-Tetramethylbutylisocyanat
Tris(2,2,6,6-tetramethylheptan-3,5-dionato-O,O')europium
2-Methylpropylisocyanid
Dichloro(ethylendiamin)platin
Phenylselenol
(Ethylenbis(nitrilomethylidyn)-2,2'-diphenolato)cobalt(II)
Kupfer(II)-4-oxopent-2-en-2-olat
Tris(2,2,6,6-tetramethylheptan-3,5-dionato-O,O')dysprosium
N-Butylisocyanid
Ethylisocyanacetat
Benzylisocyanid
(Pentan-2,4-dionato-O,O')silber
Aluminiumtris(2,4-pentandionato-O,O')
Methylisocyanacetat
(p-Toluolsulfonyl)methylisocyanid
Dehydrogenase, Aldehyd (Nicotinamidadenindinucleotid (Phosphat))
D-(+)-Glyceraldehyd
- D-GLYCERALDEHYDE 3-PHOSPHATE: NAD+ OXIDOREDUCTASE [PHOSPHORYLATING]
- D-GLYCERALDEHYDE-3-PHOSPHATE: NAD OXIDOREDUCTASE (PHOSPHORYLATING)
- EC 1.2.1.1.2
- EC: 1.2.1.12
- glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle
- G-3-PDH
- D-Glyceraldehyde 3-phosphate
- NAD+ oxidoreductase (phosphorylating)
- Dehydrogenase, glyceraldehyde phosphate
- GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE FROM RABBI
- GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE , FROM BAKE
- GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE FROM CHICK
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM HUMAN ERYTHROCYTES
- Glyceraldehyde-3-PhosphateDehydrog-
- GAPDH)/CH8530
- glyceraldehyde-3-phosphate dehydrogenase from baker's yeast (s. cerevisiae)
- glyceraldehyde-3-phosphate dehydrogenase from chicken muscle
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, SUSPENSION, FROM RABBIT MUSCLE
- GAPDH, D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating)
- Glyceraldehyde-3-phosphate Dehydrogenase Agarose from baker's yeast (S.cerevisiae)
- glyceraldehyde-3-phoshate dehydrogenase(rabbit muscle)
- phosphoglycerine aldehyde dehydrogenase
- Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
- Glyceraldehyde-3-phosphate Dehydrogenase from baker's yeast (S. cerevisiae),D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
- Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
- Glyceraldehyde-3-phosphate Dehydrogenase from chicken muscle,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
- Glyceraldehyde-3-phosphate dehydrogenase human
- Triosephosphate dehydrogenase
- cb609
- gapd
- mg:bb02e05
- GAPDH
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
- D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
- Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle lyophilized powder, >=75 units/mg protein
- Native Chicken Glyceraldehyde-3-phosphate Dehydrogenase
- Native Human Glyceraldehyde-3-phosphate Dehydrogenase
- Native Rabbit Glyceraldehyde-3-phosphate Dehydrogenase
- glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
- Native Bakers yeast (S. cerevisiae) Glyceraldehyde-3-phosphate Dehydrogenase
- Human GAPDH Protein, His Tag
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE USP/EP/BP
- Glyceraldehyde-3-phosphate dehydrogenase (NAD)
- GAPDH from rabbit muscle
- 9001-50-7
- General Metabolic Enzymes
- General Metabolic Enzymes A-H
- Molecular Biology Reagents
- Molecular Biology
- Metabolomics
- BioChemical
- Biochemicals
