M30 CytoDEATH

M30 CytoDEATH Struktur
CAS-Nr.
Englisch Name:
M30 CytoDEATH
Synonyma:
M30 CytoDEATH
CBNumber:
CB74670130
Summenformel:
Molgewicht:
0
MOL-Datei:
Mol file

M30 CytoDEATH Eigenschaften

storage temp. 
2-8°C
Aggregatzustand
lyophilized (clear, colorless solution after reconstitution)

Sicherheit

M30 CytoDEATH Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

The M30 CytoDEATH antibody is used for the determination of early apoptotic events in cells and tissue sections by detection of a specific epitope of cytokeratin 18 that is presented after cleavage by caspases. Detect apoptosis by applying the M30 antibody to fixed samples, then using secondary detection systems suitable for immunohistochemistry, immunocytochemistry, and flow cytometry.

Allgemeine Beschreibung

Mouse monoclonal antibody (Clone M30, mouse IgG2b) is used for the detection of apoptosis in epithelial cells (caspase cleavage product of cytokeratin 18). It is provided in lyophilized, stabilized, formalin grade form.
During apoptosis, vital intracellular proteins are cleaved. The proteases that mediate this process are called caspases (Cysteinyl-aspartic acid proteases). Caspases are expressed as zymogenes, which are activated by different apoptosis inducers. Once activated, a single caspase activates a cascade of caspases. Until recently, cytokeratins, in particular cytokeratin 18 (CK18), were not known to be affected by early events of apoptosis. Recently, it has been shown that the M30 antibody recognizes a specific caspase-cleavage site within cytokeratin 18, which is not detectable in native CK18 of normal cells. Consequently, the M30 CytoDEATH antibody is a unique tool for the easy and reliable determination of very early apoptotic events in single cells and tissue sections.
  • Assay time: 2 hours for immunofluorescence on cells, 3.5 hours for staining of tissues (excluding dewaxing)
  • Sample material: Adherent cells, tissue samples (routinely fixed and paraffin-embedded tissue sections, cryostat sections)

M30 CytoDEATH Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


M30 CytoDEATH Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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