Bacillus thuringiensis Chemische Eigenschaften,Einsatz,Produktion Methoden
Beschreibung
Bacillus thuringiensis or Bt is a naturally occurring rod-shaped, spore-forming, aerobic, grampositive micro-organism (bacterium) that is found throughout most areas of the world. It can be found in soils and on leaves/needles and in other common environmental situations. When the bacteria produces spores, it also produces unique crystalline proteins. When eaten, these natural proteins are toxic to certain insects, but not to human beings, birds, or other animals.
Bacillus thuringiensis is the most widely known and researched bacterium within this group and is differentiated from other spore-forming bacilli by the presence of a parasporal body that is formed within the sporangium during sporogenesis. The parasporal body is a high-molecular-mass protein crystal that is referred to as crystalline protein, δ-endotoxin, as well as a parasporal body. This protein moiety possesses some of the insecticidal properties of the bacterium.
History
Bacillus thuringiensis (Bt) is a bacterium that was first identified by S. Ishiwata in 1901 in Japanese silkworms presenting flacherie, or flaccid disease. It was later scientifically described and named by E. Berliner in Thüringen, Germany (Knowles 1994) . Berliner noted that the bacterium was producing insecticidal crystal (Cry) proteins, which were causing cell death in the digestive tract. These proteins have been used in insecticidal sprays and dusts to control pest insects since the 1930s. However, today they are most widely utilized as a transgene inserted into crop plants. These transgenic plants (Bt crops) are able to express the bacterial toxin-genes to defend themselves against herbivory. The use of these crops has been effective in controlling pest populations and increasing crop yield and quality. Bt crops were first commercialized in 1996 and, in 2013 187.5 million acres of Bt transgenic crops were planted worldwide (James et al. 2014).
Verwenden
Bt has been used in spray formulations for more than 40 years,
and more recently its insecticidal protein genes have been
incorporated into several major crops. Due to their insecticidal
activity, Cry toxins are used worldwide as bioinsecticides to
control disease-vector insect and crop pest populations.
One of the most successful applications of Bt has been the
control of lepidopteran defoliators, which are pests of coniferous
forests mainly in Canada and the United States. Bt subsp.
israelensis (Bti) is highly active against larvae of disease-vector
mosquitoes like Aedes aegypti (vector of dengue fever), Aedes
albopictus (vector of chikungunya), Simulium damnosum (vector
of onchocerciasis), and certain Anopheles species (vectors of
malaria). Bti formulations (WG, water-dispersible granule;
DT, ready-to-use tablet) have been evaluated by the World
Health Organization Pesticide Evaluation Scheme (WHOPES)
and recommended as mosquito larvicides, including their
use against mosquito larvae that develop in drinking-water
containers. Successful application of Bt is highly dependent
on proper timing, weather conditions, and dosage of spray
applications. These factors combine to determine the probability
of larvae ingesting a lethal dose.
Recently, the use of Cry toxins has increased dramatically
following the introduction of cry genes into plants. These
‘Bt crops’ have thus far proved to be an effective control
strategy, and in 2004 Bt-maize and Bt-cotton were grown on
22.4 million hectares worldwide. Such widespread use,
however, has led to concerns about the effect Bt crops may have
on the environment and on human health.
Biologische Funktion
These early experiments showed that Bt toxins needed to be activated in the gut, and it was soon discovered that the critical factors were an alkaline environment and the presence of specific proteases, which cleaved the innocuous protoxin into its active form. Once activated by proteolysis, each toxin binds to receptors in the brush border membrane and causes pores to open, disrupting them ovement of solutes across the gut epithelium and causing the influx of water. The toxins were shown to be orally lethal to caterpillars in pure form, and the pro-toxins could be converted into active toxinsin vitro, using specific pro-teases under alkaline conditions. The requirement for alkaline conditions, specific proteases and specific receptors explains why Bt is harmless to mammals (which have anacidic gut and lack the corresponding receptors) and why each toxin has a narrow host range.
Nine common pests of rice, cotton and maize that are controlled by Bt crops
Health Hazard
The insecticidal action of B.t. is attributed to protein crystals produced by the bacte-
rium. Exposures of test animals to B.t. using several routes did not produce any acute
toxicity in birds, dogs, guinea pigs, mice, or rats. Also laboratory rats when injected
with B.t.k., showed no toxic or virus-like effects. No oral toxicity was found in rats,
mice, or Japanese quail fed protein crystals from B.t. var. israelensis. Studies indicated
that after rats ate B.t., the microorganism remained in the digestive system until it was
eliminated from the body. Rabbits exposed to B.t. showed mild skin irritation and rats
showed low inhalation toxicity to B.t. In fact, chronic toxicity studies in dogs, guinea
pigs, rats, and other species of test animals showed no evidence of adverse health
effects.
The toxicity of B.t. is insect specii c. Researches have provided valuable data and
identii ed B.t. subspecies that differ in toxicity to different insects. Examples of B.t.
subspecies and the insects they affect are aizawai (moths), kurstaki (moths), israelen-
sis (mosquitoes and l ies), and tenebrionis (beetles). Also, phytotoxicity studies (plant
researches) showed B.t. genes in some crops (B.t. crops) to combat insects of corn crops,
cotton, and potatoes. B.t. must be eaten by insects to be effective and works by interfer-
ing with digestion. Insects are most sensitive to B.t. when they are larvae, an immature
life stage. Insects that eat B.t., die from hunger or infection. It does not cause disease
outbreaks in insect populations. B.t. may produce toxic chemicals that are released from
the organism
Landwirtschaftliche Anwendung
Bacillus thuringiensis (Bt) is an important insect
pathogenic bacterium commercially known as
'Thuricide' It releases toxic polypeptide crystals which
are degradable by the enzyme, protease. The bacterium is
pathogenic to the following insects: Lepidoptera, Diptera
and Coleoptera.
Bacillus thuringiensis has been exploited
commercially and its sprays have been used in the USA
since the 1930s. It is the only commercialized transgene.
The Bt toxin provides resistance against insects by
binding to specific sites in the insect gut. However, insect
resistance to Bt is also known.
Sicherheitsprofil
Low toxicity by ingestion and skincontact. When heated to decomposition it emits acridsmoke and irritating vapors.
Environmental Fate
Bt Cry and Cyt toxins belong to a class of bacterial toxins
known as pore-forming toxins (PFT) that are secreted as watersoluble
proteins undergoing conformational changes in order
to insert into, or to translocate across, cell membranes of their
host. The primary action of Cry toxins is to lyse midgut
epithelial cells in the target insect by forming pores in the apical
microvilli membrane of the cells.
Bt is ineffective against adult insects and must be eaten by
feeding larvae in order to be toxic. When ingested by insect
larvae, sporulated-Bt crystalline inclusions dissolve in the
alkaline environment of insect gut, and the solubilized inactive
protoxins are converted into protease resistant active Cry
and Cyt toxins. Toxin activation involves N-terminal, Cterminal,
and intra-molecular cleavage. The activated Cry
toxins are composed of three functional domains, a seven ahelices
bundle that is involved in membrane insertion
(domain I), and two b-sheet domains (domains II and III)
involved in receptor interactions. Once activated,
Cry toxins bind to specific receptors on the brush border
membrane of the midgut epithelium columnar cells before inserting into the membrane. Toxin insertion leads to the
formation of lytic pores in microvilli of apical membranes.
Subsequently cell lysis and disruption of the midgut epithelium
release the cell content providing the spores with
a germinating medium leading to a severe septicemia and
insect death.
Bacillus thuringiensis Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
