ANTI-U2 AUXILIARY FACTOR Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Monoclonal Anti-U2AF
65 antibody produced in mouse has been used in:
- RNA immunoprecipitation
- immunoblotting
- chromatin immunoprecipitation (ChIP)
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunocytochemistry
- immunoelectron microscopy
Allgemeine Beschreibung
Monoclonal Anti-U2AF
65 (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF
65. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF
65) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF
65kDa subunit is a non-snRNP protein component of spliceosome complexes.
Biochem/physiol Actions
U2 snRNP auxiliary factor 65 kDa (U2AF65)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF65 primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF65) and (U2AF35) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.
ANTI-U2 AUXILIARY FACTOR Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
