DIG-HIGH PRIME

DIG-HIGH PRIME Struktur
CAS-Nr.
Englisch Name:
DIG-HIGH PRIME
Synonyma:
DIG-HIGH PRIME
CBNumber:
CB8684688
Summenformel:
Molgewicht:
0
MOL-Datei:
Mol file

DIG-HIGH PRIME Eigenschaften

storage temp. 
-20°C
Aggregatzustand
solution

Sicherheit

DIG-HIGH PRIME Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

DIG-High Prime-labeled DNA probes has been used in a variety of hybridization techniques :
  • in Southern blots
  • in northern blots
  • in dot/slot blots
  • for screening of gene libraries
  • in In situ hybridizations
Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1μg total human DNA. The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots.

Allgemeine Beschreibung

DIG-High Prime has enzyme and nucleotide mixture for rapid random-primed labeling of DNA with Digoxigenin-11-dUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. DIG-labeled probes are generated at high yield within one hour or after overnight incubation.

DIG-HIGH PRIME Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


DIG-HIGH PRIME Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 2)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80

  • DIG-HIGH PRIME
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