Phosphatase, alkalisch
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- CAS-Nr.
- 9001-78-9
- Bezeichnung:
- Phosphatase, alkalisch
- Englisch Name:
- Alkaline Phosphatase
- Synonyma:
- AP;alkaline;MCAP;CIAP;AKP;IRAP;IL1F3;HUNK1;HUNKI;Ostase
- CBNumber:
- CB9690084
- Summenformel:
- C21H36N8O6
- Molgewicht:
- 496.56054
- MOL-Datei:
- 9001-78-9.mol
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Phosphatase, alkalisch Eigenschaften
- Dichte
- 1.12 g/mL at 20 °C
- storage temp.
- -20°C
- Aggregatzustand
- suspension
- Farbe
- white
- Wichte
- 1
- Wasserlöslichkeit
- It is soluble in water.
- Stabilität:
- Stability Incompatible with strong oxidizing agents
- EPA chemische Informationen
- Alkaline phosphatase (9001-78-9)
Sicherheit
- Risiko- und Sicherheitserklärung
- Gefahreninformationscode (GHS)
| Kennzeichnung gefährlicher |
B,Xi |
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| R-Sätze: |
36/37/38 |
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| S-Sätze: |
26-36-24/25-22-23 |
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| WGK Germany |
3 |
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| F |
3-10 |
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| TSCA |
Yes |
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| HS Code |
35079090 |
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| Toxizität |
An enzyme with an
alkaline pH optimum that hydrolyzes phosphate monoesters.
Elevation of its activity in the serum usually indicates obstructive
jaundice, Paget’s disease (osteitis deformans), or bone
carcinoma. |
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| Bildanzeige (GHS) |
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| Alarmwort |
Warnung |
| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H317 |
Kann allergische Hautreaktionen verursachen. |
Sensibilisierung der Haut |
Kategorie 1A |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
P261, P272, P280, P302+P352,P333+P313, P321, P363, P501 |
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| Sicherheit |
| P280 |
Schutzhandschuhe/Schutzkleidung/Augenschutz tragen. |
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Phosphatase, alkalisch Chemische Eigenschaften,Einsatz,Produktion Methoden
R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
S22:Staub nicht einatmen.
S23:Gas/Rauch/Dampf/Aerosol nicht einatmen(geeignete Bezeichnung(en) vom Hersteller anzugeben).
Chemische Eigenschaften
solid
Verwenden
Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
Allgemeine Beschreibung
Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.
läuterung methode
The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]
Phosphatase, alkalisch Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
Phosphatase, alkalisch Anbieter Lieferant Produzent Hersteller Vertrieb Händler.
Global( 212)Lieferanten
9001-78-9(Phosphatase, alkalisch)Verwandte Suche:
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- PHOSPHATASE, ALKALINE TYPE XXX FROM*CALF INTESTINAL
- ALKALINE PHOSPHATASE YELLOW LI
- PHOSPHATASE ALKALINE TYPE XV FROM BOVINE PLACENTA
- PHOSPHATASE, ALKALINE TYPE XVIII FROM*PO RCINE INTES
- PHOSPHATASE ALK. FROM CALF INTEST. MUC., STAB., ~1000 U/MG*
- PHOSPHATASE ALKALINE TYPE VII-S FROM*BOV INE INTESTI
- PHOSPHATASE, ALKALINE TYPE VII-NT
- PHOSPHATASE, ALKALINE FROM SHRIMP
- PHOSPHATASE ALKALINE TYPE XXIII*FROM TRO UT INTESTIN
- ALKALINE PHOSPHATASE REAGENT
- PHOSPHATASE ALKALINE TYPE VII-L FROM*BOV INE INTESTI
- PHOSPHATASE, ALKALINE, BOVINE*RECOMBINANT PRODUCED
- PHOSPHATASE ALKALINE TYPE VII-T FROM*BOV INE INTESTI
- PHOSPHATASE ALKALINE TYPE XVI*FROM PORCI NE PLACENTA
- PHOSPHATASE ALKALINE TYPE XVII*FROM HUMA N PLACENTA
- PHOSPHATASE, ALKALINE TYPE XXXIII FROM*B OVINE LIVER
- PHOSPHATASE, ALKALINE TYPE III-L*BACTERI AL (ESCHERI
- PHOSPHATASE ALKALINE TYPE XXIV*FROM HUMA N PLACENTA
- PHOSPHATASE, ALKALINE TYPE VII-NA
- ALKALINE PHOSPHATASE MOLECULAR BIOLOGYRE AGENT
- PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, PWD.~1 U/MG
- PHOSPHATASE, ALKALINE TYPE III*BACTERIAL
- PHOSPHATASE, ALKALINE FROM BOVINE CALF*I NTESTINE
- PHOSPHATASE ALKALINE TYPE VIII*FROM RABB IT INTESTIN
- PHOSPHATASE, ALKALINE FROM BOVINE*INTEST INAL MUCOSA
- PHOSPHATASE ALKALINE TYPE XXXII FROM*BOV INE KIDNEY
- PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, ~4200 U/MG
- PHOSPHATASE, ALKALINE FROM PORCINE*KIDNE Y
- BASIC PHOSPHATASE, WHITE LYOPHILIZATED POWDER
- Phosphatase,AlkalineExCalfIntestine
- Alkaline phosphohydrolase
- Alkaline phosphomonoesterase
- Ostase
- Einecs 232-631-4
- AlkalinePhosphatase,(AmmoniumSulfateSuspensionCalfIntestine)
- ALKALINE PHOSPHATASE, RECOMBINANT, MOLECULAR BIOLOGY GRADE
- ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT
- Alkaline Phosphatase from calf intestine ca. 3 000 U/mg protein (ca. 60 U/
- Alkaline Phosphatase from calf intestine ca. 5 400 U/mg protein
- Alkaline Phosphatase from calf intestine ca. 7000 U/mg protein
- alkaline phosphatase from calf intestinal mucosa
- phosphatase, alkaline from escherichia coli
- phosphatase, alkaline from bovine liver
- phosphatase alkaline from calf intestinal mucosa
- phosphatase, alkaline from porcine intestinal mucosa
- phosphatase, alkaline from rabbit intestine
- phosphatase, alkaline from human placenta
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-L
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-S
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE I-S
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE IV
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VIII
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-L
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-S
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-T
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XVII
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXIV
- PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXXII
