ChemicalBook
Chinese english Germany Japanese Korea

Phosphatase, alkalisch Produkt Beschreibung

Alkaline Phosphatase Struktur
9001-78-9
CAS-Nr.
9001-78-9
Bezeichnung:
Phosphatase, alkalisch
Englisch Name:
Alkaline Phosphatase
Synonyma:
AP;CAP;AKP;CIAP;MCAP;IRAP;HUNK1;HUNKI;IL1F3;Ostase
CBNumber:
CB9690084
Summenformel:
C21H36N8O6
Molgewicht:
496.56054
MOL-Datei:
9001-78-9.mol

Phosphatase, alkalisch Eigenschaften

Dichte
1.12 g/mL at 20 °C
storage temp. 
2-8°C
Aggregatzustand
suspension
Farbe
white
Wichte
1
Wasserlöslichkeit
It is soluble in water.
Stabilität:
Stability Incompatible with strong oxidizing agents

Sicherheit

Kennzeichnung gefährlicher B,Xi
R-Sätze: 36/37/38
S-Sätze: 26-36-24/25-22-23
WGK Germany  3
3-10
TSCA  Yes
HS Code  35079090

Phosphatase, alkalisch Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:

R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.

S-Sätze Betriebsanweisung:

S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
S22:Staub nicht einatmen.
S23:Gas/Rauch/Dampf/Aerosol nicht einatmen(geeignete Bezeichnung(en) vom Hersteller anzugeben).

Chemische Eigenschaften

solid

Verwenden

Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.

Verwenden

Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.

läuterung methode

The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]

Phosphatase, alkalisch Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


Phosphatase, alkalisch Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 114)Lieferanten
Firmenname Telefon Fax E-Mail Land Produktkatalog Edge Rate
Henan DaKen Chemical CO.,LTD.
+86-371-55531817
info@dakenchem.com CHINA 21671 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com CHINA 20672 55
career henan chemical co
+86-371-86658258
sales@coreychem.com CHINA 30002 58
Hubei xin bonus chemical co. LTD
86-13657291602
027-59338440 sales@guangaobio.com CHINA 23049 58
BOC Sciences
1-631-619-7922
1-631-614-7828 inquiry@bocsci.com United States 20115 58
Chongqing Chemdad Co., Ltd
+86-13650506873
sales@chemdad.com CHINA 35440 58
Hefei Bomei Biotechnology Co., Ltd. 13739298932
531626407@qq.com CHINA 3007 58
J & K SCIENTIFIC LTD. 400-666-7788 +86-10-82848833
86-10-82849933 jkinfo@jkchemical.com;market6@jkchemical.com China 96815 76
Meryer (Shanghai) Chemical Technology Co., Ltd. +86-(0)21-61259100(Shanghai) +86-(0)755-86170099(ShenZhen) +86-(0)10-62670440(Beijing)
+86-(0)21-61259102(Shanghai) +86-(0)755-86170066(ShenZhen) +86-(0)10-88580358(Beijing) sh@meryer.com China 40269 62
Energy Chemical 021-58432009 / 400-005-6266
021-58436166-800 info@energy-chemical.com China 44025 61

9001-78-9(Phosphatase, alkalisch)Verwandte Suche:


  • alkaline
  • phosphatasealkalinefrome.coli
  • ALKALINE PHOSPHATASE, SHRIMP
  • ALKALINE PHOSPHATASE, BOVINE
  • ALKALINE PHOSPHATASE CALF INTESTINAL
  • EC 3.1.3.1
  • EC: 3.1.3.1
  • CALF INTESTINAL ALKALINE PHOSPHATASE
  • CALF INTESTINE ALKALINE PHOSPHATASE
  • CAP
  • CIAP
  • AP
  • IUB: 3.1.3.1
  • SHRIMP ALKALINE PHOSPHATASE
  • ORTHOPHOSPHORIC-MONOESTER PHOSPHOHYDROLASE
  • ORTHOPHOSPHORIC-MONOESTER PHOSPHOHYDROLASE (ALKALINE OPTIMUM)
  • ORTHOPHOSPHORIC-MONOESTER PHOSPHOHYDROLASE (ALKALINE OPTIMUM) BOVINE
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM]
  • PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III
  • phosphatase alkaline type xvii*from human placent
  • PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, ~2500 U/MG
  • PHOSPHATASE ALKALINE TYPE XII FROM*SHEEP INTESTINE
  • PHOSPHATASE, ALKALINE TYPE I-S FROM*BOVI NE INTESTIN
  • PHOSPHATASE ALKALINE F. BOV. INTESTINAL MUCOSA, ~2000 U/MG
  • ALKALINE PHOSPHATASE (CATALYTIC CONCENTR
  • PHOSPHATASE, ALKALINE TYPE XXX FROM*CALF INTESTINAL
  • ALKALINE PHOSPHATASE YELLOW LI
  • PHOSPHATASE ALKALINE TYPE XV FROM BOVINE PLACENTA
  • PHOSPHATASE, ALKALINE TYPE XVIII FROM*PO RCINE INTES
  • PHOSPHATASE ALK. FROM CALF INTEST. MUC., STAB., ~1000 U/MG*
  • PHOSPHATASE ALKALINE TYPE VII-S FROM*BOV INE INTESTI
  • PHOSPHATASE, ALKALINE TYPE VII-NT
  • PHOSPHATASE, ALKALINE FROM SHRIMP
  • PHOSPHATASE ALKALINE TYPE XXIII*FROM TRO UT INTESTIN
  • ALKALINE PHOSPHATASE REAGENT
  • PHOSPHATASE ALKALINE TYPE VII-L FROM*BOV INE INTESTI
  • PHOSPHATASE, ALKALINE, BOVINE*RECOMBINANT PRODUCED
  • PHOSPHATASE ALKALINE TYPE VII-T FROM*BOV INE INTESTI
  • PHOSPHATASE ALKALINE TYPE XVI*FROM PORCI NE PLACENTA
  • PHOSPHATASE ALKALINE TYPE XVII*FROM HUMA N PLACENTA
  • PHOSPHATASE, ALKALINE TYPE XXXIII FROM*B OVINE LIVER
  • PHOSPHATASE, ALKALINE TYPE III-L*BACTERI AL (ESCHERI
  • PHOSPHATASE ALKALINE TYPE XXIV*FROM HUMA N PLACENTA
  • PHOSPHATASE, ALKALINE TYPE VII-NA
  • ALKALINE PHOSPHATASE MOLECULAR BIOLOGYRE AGENT
  • PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, PWD.~1 U/MG
  • PHOSPHATASE, ALKALINE TYPE III*BACTERIAL
  • PHOSPHATASE, ALKALINE FROM BOVINE CALF*I NTESTINE
  • PHOSPHATASE ALKALINE TYPE VIII*FROM RABB IT INTESTIN
  • PHOSPHATASE, ALKALINE FROM BOVINE*INTEST INAL MUCOSA
  • PHOSPHATASE ALKALINE TYPE XXXII FROM*BOV INE KIDNEY
  • PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, ~4200 U/MG
  • PHOSPHATASE, ALKALINE FROM PORCINE*KIDNE Y
  • BASIC PHOSPHATASE, WHITE LYOPHILIZATED POWDER
  • Phosphatase,AlkalineExCalfIntestine
  • Alkaline phosphohydrolase
  • Alkaline phosphomonoesterase
  • Ostase
Copyright 2019 © ChemicalBook. All rights reserved