Z-VAD-FMK
- CAS No.
- 187389-52-2
- Chemical Name:
- Z-VAD-FMK
- Synonyms
- CS-2005;CS-1507;Z-VAD-FMK;HGNC:1504;Z-VAD(OME)-FMK;Z-VA(OME)D-FMK;Z-VADC(OME)-FMK;Z-VAD-FMK, >=98%;Z-VAL-ALA-ASP-FMK;CASPASE INHIBITOR I
- CBNumber:
- CB0203438
- Molecular Formula:
- C22H30FN3O7
- Molecular Weight:
- 467.49
- MDL Number:
- MFCD03452883
- MOL File:
- Mol file
- MSDS File:
- SDS
| Melting point | >190°C (dec.) |
|---|---|
| Boiling point | 732.4±60.0 °C(Predicted) |
| Density | 1.214±0.06 g/cm3 (20 ºC 760 Torr) |
| Flash point | 396.7℃ |
| storage temp. | -20°C |
| solubility | DMSO: 20 mg/mL |
| form | powder |
| pka | 11.05±0.46(Predicted) |
| color | white |
| Stability | Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week. |
| InChIKey | MIFGOLAMNLSLGH-QOKNQOGYSA-N |
| SMILES | C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(OC)=O)C(=O)CF |
| CAS DataBase Reference | 187389-52-2 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H315-H319 |
| Precautionary statements | P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313 |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| HS Code | 29145090 |
Z-VAD-FMK price More Price(37)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | V116 | Z-VAD-FMK solid | 187389-52-2 | 2mg | $814 | 2024-03-01 | Buy |
| Sigma-Aldrich | SAB2501669 | Anti-CASP3 antibody produced in goat affinity isolated antibody, buffered aqueous solution | 187389-52-2 | 100μg | $568 | 2024-03-01 | Buy |
| Sigma-Aldrich | 627610 | Caspase Inhibitor I | 187389-52-2 | 1mg | $318 | 2024-03-01 | Buy |
| Sigma-Aldrich | 627610 | Caspase Inhibitor I | 187389-52-2 | 5mg | $662 | 2024-03-01 | Buy |
| Cayman Chemical | 14463 | Z-VAD(OMe)-FMK ≥95% | 187389-52-2 | 1mg | $100 | 2024-03-01 | Buy |
Z-VAD-FMK Chemical Properties,Uses,Production
Description
Z-VAD-FMK (187389-52-2), cell-permeable, methyl ester form. Potent, irreversible pan-caspase inhibitor. Inhibits caspase activity and apoptosis induction in a variety of cell types (IC50 = 1.5 μM). Active in vivo.
Uses
Z-VAD(OMe)-FMK is a cell-permeable, competitive, and irreversible inhibitor of all caspases. Through this action, it inhibits cleavage of poly(ADP-ribose) polymerase, preventing apoptosis when used at 10-50 μM. It also blocks caspase-mediated apoptosis in vivo. Z-VAD(OMe)-FMK effectively prevents caspase action in inflammasomes.[Cayman Chemical]
Uses
Z-VAD-FMK is a caspase inhibitor that inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentrations of z-FA-CMK.
Definition
ChEBI: Z-Val-Ala-Asp(OMe)-CH2F is a tripeptide consisting of Z-Val-Ala-Asp(OMe) in which the C-terminal OH group has been replaced by a fluoromethyl group. An irreversible pan-caspase inhibitor. It has a role as an apoptosis inhibitor and a protease inhibitor. It is a carbamate ester, a tripeptide and an organofluorine compound.
General Description
A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.
Biological Activity
Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC 50 = 0.0015-5.8 mM). Active in vivo .
Biochem/physiol Actions
Cell permeable: yes
Enzyme inhibitor
This tripeptide halomethyl ketone (FWfree-acid = 467.49 g/mol; Soluble to 9.35 mg/ml in DMSO; CAS RegistryNumber = 187389-52-2), also known as Z-VAD-FMK and caspase inhibitor VI, is a broad-spectrum caspase inhibitor that blocks caspase-mediated apoptosis. Z-VAD-FMK inhibits caspase processing (IC50 = 0.0015–5.8 mM, depending on enzyme and cell type). The aspartate methyl ester derivative (FW = 467.49 g/mol), also known as caspase inhibitor I, is far more cell-permeable. Target (s) : caspases; caspase-1; caspase-2; caspase-3; caspase-4 ; caspase 5; caspase-6; caspase-7; caspase-8 ; caspase-9; caspase-10.
storage
-20°C
References
1) Slee et al. (1996), Benzyloxycarbonyl-Val-Ala-ASP (OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32; Biochem. J., 315 21 2) Kunstle et al. (1997), ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-alpha; Immunol. Lett., 55 5 3) Garcia-Calvo et al. (1998), Inhibition of human caspases by peptide based and macromolecular inhibitors; J. Biol. Chem., 273 32608
Z-VAD-FMK Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| BOC Sciences | +16314854226 | inquiry@bocsci.com | United States | 19743 | 58 |
| Hangzhou FandaChem Co.,Ltd. | 008657128800458; +8615858145714 | fandachem@gmail.com | China | 9352 | 55 |
| ATK CHEMICAL COMPANY LIMITED | +undefined-21-51877795 | ivan@atkchemical.com | China | 32480 | 60 |
| career henan chemical co | +86-0371-86658258 | sales@coreychem.com | China | 29914 | 58 |
| Biochempartner | 0086-13720134139 | candy@biochempartner.com | CHINA | 967 | 58 |
| Accela ChemBio Inc. | (+1)-858-699-3322 | info@accelachem.com | United States | 19965 | 58 |
| Shanghai Longyu Biotechnology Co., Ltd. | +8615821988213 | info@longyupharma.com | China | 2531 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-61398051 +8613650506873 | sales@chemdad.com | China | 39916 | 58 |
| HONG KONG IPURE BIOLOGY CO.,LIMITED | 86 18062405514 18062405514 | ada@ipurechemical.com | CHINA | 3465 | 58 |
| HANGZHOU CLAP TECHNOLOGY CO.,LTD | 86-571-88216897,88216896 13588875226 | sales@hzclap.com | CHINA | 6313 | 58 |
